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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02935 [old locus tag: SA0501 ]
  • pan locus tag?: SAUPAN002315000
  • symbol: SA_RS02935
  • pan gene symbol?: rpoC
  • synonym:
  • product: DNA-directed RNA polymerase subunit beta'

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02935 [old locus tag: SA0501 ]
  • symbol: SA_RS02935
  • product: DNA-directed RNA polymerase subunit beta'
  • replicon: chromosome
  • strand: +
  • coordinates: 583335..586931
  • length: 3597
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (583335..586931) NCBI
  • BioCyc: G1G21-572 BioCyc
  • MicrobesOnline: see SA0501

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
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    901
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    1021
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    1141
    1201
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    1321
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    1441
    1501
    1561
    1621
    1681
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    1801
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    1981
    2041
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    3241
    3301
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    3541
    ATGAAAATAGGATTGGCTTCACCTGAAAAAATCCGTTCTTGGTCTTTTGGTGAAGTTAAA
    AAACCTGAAACAATCAACTACCGTACATTAAAACCTGAAAAAGATGGTCTATTCTGTGAA
    AGAATTTTCGGACCTACAAAAGACTGGGAATGTAGTTGTGGTAAATACAAACGTGTTCGC
    TACAAAGGCATGGTCTGTGACAGATGTGGAGTTGAAGTAACTAAATCTAAAGTACGTCGT
    GAAAGAATGGGTCACATTGAACTTGCTGCTCCAGTTTCTCACATTTGGTATTTCAAAGGT
    ATACCAAGTCGTATGGGATTATTACTTGACATGTCACCAAGAGCATTAGAAGAGGTTATT
    TACTTTGCTTCTTATGTTGTTGTAGATCCAGGTCCAACTGGTTTAGAAAAGAAAACTTTA
    TTATCTGAAGCTGAATTCAGAGATTATTATGATAAATACCCAGGTCAATTCGTTGCAAAA
    ATGGGTGCAGAAGGTATTAAAGATTTACTTGAAGAGATTGATCTTGACGAAGAACTTAAA
    TTGTTACGCGATGAGTTGGAATCAGCTACTGGTCAAAGACTTACTCGTGCAATTAAACGT
    TTAGAAGTTGTTGAATCATTCCGTAATTCAGGTAACAAACCTTCATGGATGATTTTAGAT
    GTACTTCCAATCATCCCACCAGAAATTCGTCCAATGGTTCAATTAGATGGTGGACGATTT
    GCAACAAGTGACTTAAACGACTTATACCGTCGTGTAATTAATCGAAATAATCGTTTGAAA
    CGTTTATTAGATTTAGGTGCACCTGGTATCATCGTTCAAAACGAAAAACGTATGTTACAA
    GAAGCCGTTGACGCTTTAATTGATAATGGTCGTCGTGGTCGTCCAGTTACTGGCCCAGGT
    AACCGTCCATTAAAATCTTTATCTCATATGTTAAAAGGTAAACAAGGTCGTTTCCGTCAA
    AACTTACTTGGTAAACGTGTCGACTATTCAGGACGTTCAGTTATTGCAGTAGGTCCAAGC
    TTGAAAATGTACCAATGTGGTTTACCAAAAGAAATGGCACTTGAACTATTTAAACCATTC
    GTAATGAAAGAATTAGTTCAACGTGAAATTGCAACTAACATTAAAAATGCGAAGAGTAAA
    ATCGAACGTATGGATGATGAAGTTTGGGACGTATTGGAAGAAGTAATTAGAGAACATCCT
    GTATTACTTAACCGTGCACCAACACTTCATAGACTTGGTATTCAAGCATTTGAACCAACT
    TTAGTTGAAGGTCGTGCGATTCGTCTACATCCACTTGTAACAACAGCTTATAATGCTGAC
    TTTGACGGTGACCAAATGGCGGTTCACGTTCCTTTATCAAAAGAGGCACAAGCTGAAGCA
    AGAATGTTGATGTTAGCAGCACAAAACATCTTGAACCCTAAAGATGGTAAACCTGTAGTT
    ACACCATCACAAGATATGGTACTTGGTAACTATTACCTTACTTTAGAAAGAAAAGATGCA
    GTAAATACAGGCGCAATCTTTAATAATACAAATGAAGTATTAAAAGCATATGCAAATGGC
    TTTGTACATTTACACACTAGAATTGGTGTACATGCAAGTTCGTTCAATAATCCAACATTT
    ACTGAAGAACAAAACAAAAAGATTCTTGCTACGTCAGTAGGTAAAATTATATTCAATGAA
    ATCATTCCAGATTCATTTGCTTATATTAATGAACCTACGCAAGAAAACTTAGAAAGAAAG
    ACACCAAACAGATATTTCATCGATCCTACAACTTTAGGTGAAGGTGGATTAAAAGAATAC
    TTTGAAAATGAAGAATTAATTGAACCTTTCAACAAAAAATTCTTAGGTAATATTATTGCA
    GAAGTATTCAACAGATTTAGCATCACTGATACATCAATGATGTTAGACCGTATGAAAGAC
    TTAGGATTCAAATTCTCATCTAAAGCTGGTATTACAGTAGGTGTTGCTGATATCGTAGTA
    TTACCTGATAAGCAACAAATACTTGATGAGCATGAAAAATTAGTCGACAGAATTACAAAA
    CAATTCAACCGTGGTTTAATCACTGAAGAAGAAAGATATAATGCAGTTGTTGAAATTTGG
    ACAGATGCAAAAGATCAAATTCAAGGTGAATTGATGCAATCACTTGATAAAACTAACCCA
    ATCTTCATGATGAGTGATTCAGGTGCCCGTGGTAACGCATCTAACTTTACACAGTTAGCA
    GGTATGCGTGGATTGATGGCCGCACCATCTGGTAAGATTATCGAATTACCAATCACATCT
    TCATTCCGTGAAGGTTTAACAGTACTTGAATACTTCATCTCAACTCACGGTGCACGTAAA
    GGTCTTGCCGATACAGCACTTAAAACAGCTGACTCAGGATATCTTACTCGTCGTCTTGTT
    GACGTGGCACAAGATGTTATTGTTCGTGAAGAAGACTGTGGTACTGATAGAGGTTTATTA
    GTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGT
    CGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGAT
    GAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTGAACAAATGTAT
    ATTCGCTCAGCATTTACTTGTAACGCACGACATGGTGTTTGTGAAAAATGTTACGGTAAA
    AACCTTGCTACTGGTGAAAAAGTTGAAGTTGGTGAAGCAGTTGGTACAATTGCAGCCCAA
    TCTATCGGTGAACCAGGTACACAGCTTACAATGCGTACATTCCATACAGGTGGGGTAGCA
    GGTAGCGATATCACACAAGGTCTTCCTCGTATTCAAGAGATTTTCGAAGCACGTAACCCT
    AAAGGTCAAGCGGTAATTACGGAAATCGAAGGTGTCGTAGAAGATATTAAATTAGCAAAA
    GATAGACAACAAGAAATTGTTGTTAAAGGTGCTAATGAAACAAGATCATACCTTGCTTCA
    GGTACTTCAAGAATTATTGTAGAAATCGGTCAACCAGTTCAACGTGGTGAAGTATTAACT
    GAAGGTTCTATTGAACCTAAGAATTACTTATCTGTTGCTGGATTAAACGCGACTGAAAGC
    TACTTATTAAAAGAAGTACAAAAAGTTTACCGTATGCAAGGTGTAGAAATCGACGATAAA
    CACGTTGAGGTTATGGTTCGACAAATGTTACGTAAAGTTAGAATTATCGAAGCAGGTGAT
    ACGAAGTTATTACCAGGTTCATTAGTTGATATTCATAACTTTACAGATGCAAATAGAGAA
    GCATTTAAACACCGTAAGCGTCCTGCAACAGCTAAACCAGTATTACTTGGTATTACTAAA
    GCATCACTTGAAACAGAAAGTTTCTTATCTGCAGCATCATTCCAAGAAACAACAAGAGTT
    CTTACAGATGCAGCAATTAAAGGTAAGCGTGATGACTTATTAGGTCTTAAAGAAAACGTA
    ATTATTGGTAAGTTAATTCCAGCTGGTACTGGTATGAGACGTTATAGCGACGTAAAATAC
    GAAAAAACAGCTAAACCAGTTGCAGAAGTTGAATCTCAAACTGAAGTAACGGAATAA
    60
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    3240
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    3420
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    3597

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS02935 [old locus tag: SA0501 ]
  • symbol: SA_RS02935
  • description: DNA-directed RNA polymerase subunit beta'
  • length: 1198
  • theoretical pI: 7.01614
  • theoretical MW: 134273
  • GRAVY: -0.354341

Function[edit | edit source]

  • reaction:
    EC 2.7.7.6?  ExPASy
    DNA-directed RNA polymerase Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
  • TIGRFAM:
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta' subunit (TIGR02386; EC 2.7.7.6; HMM-score: 1883.6)
    and 4 more
    DNA-directed RNA polymerase, gamma subunit (TIGR02387; EC 2.7.7.6; HMM-score: 843.5)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta subunit (TIGR02388; EC 2.7.7.6; HMM-score: 645.3)
    DNA-directed RNA polymerase subunit A' (TIGR02390; EC 2.7.7.6; HMM-score: 320.8)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, subunit A (TIGR02389; EC 2.7.7.6; HMM-score: 115)
  • TheSEED: see SA0501
  • PFAM:
    no clan defined RNA_pol_Rpb1_1; RNA polymerase Rpb1, domain 1 (PF04997; HMM-score: 294.8)
    and 5 more
    RNA_pol_Rpb1_5; RNA polymerase Rpb1, domain 5 (PF04998; HMM-score: 233.5)
    RNA_pol_Rpb1_2; RNA polymerase Rpb1, domain 2 (PF00623; HMM-score: 140.1)
    RNA_pol_Rpb1_3; RNA polymerase Rpb1, domain 3 (PF04983; HMM-score: 117.5)
    RNA_pol_Rpb1_4; RNA polymerase Rpb1, domain 4 (PF05000; HMM-score: 54.7)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 17.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.146282
    • TAT(Tat/SPI): 0.006504
    • LIPO(Sec/SPII): 0.007747
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFGPTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIPSRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAKMGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMILDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSVIAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVLEEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHVPLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFNNTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSFAYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFNRFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQFNRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLAGMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEIIIRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGVVEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYLSVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSLVDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAIKGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS00170DNA gyrase subunit A  [2] (data from MRSA252)
    SA_RS014452-C-methyl-D-erythritol 4-phosphate cytidylyltransferase  [2] (data from MRSA252)
    SA_RS0295030S ribosomal protein S7  [2] (data from MRSA252)
    SA_RS02955elongation factor G  [2] (data from MRSA252)
    SA_RS02960elongation factor Tu  [2] (data from MRSA252)
    SA_RS05895isoleucine--tRNA ligase  [2] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [2] (data from MRSA252)
    SA_RS07335hypothetical protein  [2] (data from MRSA252)
    SA_RS07695elongation factor P  [2] (data from MRSA252)
    SA_RS08505aldehyde dehydrogenase  [2] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [2] (data from MRSA252)
    SA_RS10535molecular chaperone GroEL  [2] (data from MRSA252)
    SA_RS11145purine-nucleoside phosphorylase  [2] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [2] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [2] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [2] (data from MRSA252)
    SA_RS12040octopine dehydrogenase  [2] (data from MRSA252)
    SA_RS13920arginine deiminase  [2] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]