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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03000 [old locus tag: SA0514 ]
  • pan locus tag?: SAUPAN002329000
  • symbol: SA_RS03000
  • pan gene symbol?: dck
  • synonym:
  • product: deoxynucleoside kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03000 [old locus tag: SA0514 ]
  • symbol: SA_RS03000
  • product: deoxynucleoside kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 601373..602035
  • length: 663
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (601373..602035) NCBI
  • BioCyc: SA_RS03000 BioCyc
  • MicrobesOnline: see SA0514

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAATTACGGTATTCCACAAAATGCCATTATAACCATTGCAGGTACAGTTGGTGTT
    GGAAAATCAACACTAACGCAAGCACTTGCAGATAAATTAAACTTTAAAACGTCTTTTGAA
    AATGTCGAACATAATCCATATTTAGATAAATTTTATAGCGATTTTGAACGATGGAGTTTC
    CATTTGCAAATTTACTTCTTAGCTGAACGTTTTAAAGAACAAAAGCGTATGTTTGAATAT
    GGTGGTGGCTTTGTCCAAGATCGATCAATTTATGAAGATGTTGATATTTTTGCAAAAATG
    CATGAAGAAGAAGGCACAATGAGTAAAGAAGATTTCAAAACATATTCAGACTTATTTAAT
    GCCATGGTCATGACACCTTATTTTCCTAAACCAGATGTAATGATTTATTTAGAATGTAAC
    TATGATGAGGTCATTGATCGTATTATTGAACGTGGTCGCGAAATGGAAATTAATACAGAC
    CCTGAATACTGGAAAAAGCTATTTAAACGCTATGACGATTGGATTAATAGCTTTAATGCA
    TGTCCAGTTGTACGTATCAATATTAATGAATATGATATCCATAAGGACCCCGAATCTTTA
    AATCCTATGATAGACAAAATTGCTCGAATTATTCAAACATATCGACAAGTAGATACACGA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03000 [old locus tag: SA0514 ]
  • symbol: SA_RS03000
  • description: deoxynucleoside kinase
  • length: 220
  • theoretical pI: 4.72394
  • theoretical MW: 26228.5
  • GRAVY: -0.58

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions dTMP kinase (TIGR00041; EC 2.7.4.9; HMM-score: 31.3)
    and 17 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 21.2)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A dephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 18.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 17.8)
    Genetic information processing Protein synthesis tRNA aminoacylation L-seryl-tRNA(Sec) kinase (TIGR03574; EC 2.7.1.164; HMM-score: 17.5)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine kinase (TIGR00235; EC 2.7.1.48; HMM-score: 15.1)
    Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 14.1)
    nicotinamide-nucleotide adenylyltransferase (TIGR01526; EC 2.7.7.1; HMM-score: 13.8)
    UMP-CMP kinase family (TIGR01359; EC 2.7.4.-; HMM-score: 13.5)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 13)
    adenylate kinase (TIGR01360; EC 2.7.4.3; HMM-score: 12.7)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 12.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions adenylate kinase (TIGR01351; EC 2.7.4.-; HMM-score: 11.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dimethylallyltransferase (TIGR00174; EC 2.5.1.75; HMM-score: 11.4)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 11.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium chelatase ATPase subunit D (TIGR02031; EC 6.6.1.1; HMM-score: 10.2)
  • TheSEED: see SA0514
  • PFAM:
    P-loop_NTPase (CL0023) dNK; Deoxynucleoside kinase (PF01712; HMM-score: 211)
    and 31 more
    AAA_18; AAA domain (PF13238; HMM-score: 46.2)
    Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 23.8)
    AAA_17; AAA domain (PF13207; HMM-score: 22.8)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 20.3)
    AAA_28; AAA domain (PF13521; HMM-score: 20.2)
    Cytidylate_kin2; Cytidylate kinase-like family (PF13189; HMM-score: 17.9)
    ABC_tran; ABC transporter (PF00005; HMM-score: 17.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 17.6)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 16.9)
    AAA_22; AAA domain (PF13401; HMM-score: 16.9)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 15.3)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 14.8)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.5)
    AAA_33; AAA domain (PF13671; HMM-score: 14.5)
    ATPase; KaiC (PF06745; HMM-score: 14.4)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.3)
    SKI; Shikimate kinase (PF01202; HMM-score: 14.1)
    AAA_14; AAA domain (PF13173; HMM-score: 14)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 13.7)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.3)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 12.5)
    PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 12.5)
    AAA_25; AAA domain (PF13481; HMM-score: 12.4)
    NACHT; NACHT domain (PF05729; HMM-score: 12.3)
    no clan defined DUF1087; Domain of Unknown Function (DUF1087) (PF06465; HMM-score: 12.3)
    P-loop_NTPase (CL0023) NTPase_1; NTPase (PF03266; HMM-score: 12.2)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 12)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 11.8)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 11.8)
    ParA; NUBPL iron-transfer P-loop NTPase (PF10609; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.320581
    • TAT(Tat/SPI): 0.01874
    • LIPO(Sec/SPII): 0.009548
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEHNPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHEEEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTDPEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVDTR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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