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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03595
  • pan locus tag?:
  • symbol: SA_RS03595
  • pan gene symbol?:
  • synonym:
  • product: LysR family transcriptional regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03595
  • symbol: SA_RS03595
  • product: LysR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 723333..724199
  • length: 867
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_002745 (723333..724199) NCBI
  • BioCyc: SA_RS03595 BioCyc
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAGATTGAAGACTATCGTTTACTAATAACATTAGACGAAACGAAAACGTTACGTAAA
    GCGGCTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAA
    AATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAA
    GGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGAC
    AAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTG
    ATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGCCCAATTTCCTAATGTT
    GAAATACAAGTGCAAGTTGGTTCAACTGAACAAATTAAAGCAAATCATAGAGATTATCAT
    GTTATGATAACTCGTGGAAATAAGGTAATGAATTTAGCTAACACACATTTATTTAATGAT
    GATCATTATTTTATTTTTCCAAAAAATAGACGAGATGATGTTACAAAGTTACCATTTATA
    GAGTTTCAAGCTGATCCGATTTATATAAATCAAATAAAACAATGGTATAACGATAATTTA
    GAACAAGATTACCATGCAACTATTACAGTGGATCAAGTAGCAACTTGCAAAGAAATGTTG
    ATTAGTGGTGTAGGTGTTACAATTTTGCCGGAAATTATGATGAAAAATATCAGCAAAGAA
    CAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATG
    AGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCG
    AGCTTTGTTGAACAACCAAAGGCGTAG
    60
    120
    180
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    867

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS03595
  • symbol: SA_RS03595
  • description: LysR family transcriptional regulator
  • length: 288
  • theoretical pI: 5.40473
  • theoretical MW: 33243.1
  • GRAVY: -0.219097

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 74.4)
    and 7 more
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 54.4)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 54.4)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 47.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 47.7)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 47.7)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 41.3)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 33.1)
  • TheSEED:
  • PFAM:
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 83.2)
    and 3 more
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 53.8)
    HTH_30; PucR C-terminal helix-turn-helix domain (PF13556; HMM-score: 19.9)
    no clan defined DUF1433; Protein of unknown function (DUF1433) (PF07252; HMM-score: 15.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003248
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000327
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 446610066 NCBI
  • RefSeq: WP_000687412 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFGVDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIGQTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFNDDHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKEMLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFVNLMASFVEQPKA

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]