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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03735 [old locus tag: SA0655 ]
  • pan locus tag?: SAUPAN002584000
  • symbol: SA_RS03735
  • pan gene symbol?: fruA
  • synonym:
  • product: PTS fructose transporter subunit IIC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03735 [old locus tag: SA0655 ]
  • symbol: SA_RS03735
  • product: PTS fructose transporter subunit IIC
  • replicon: chromosome
  • strand: +
  • coordinates: 748015..749973
  • length: 1959
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (748015..749973) NCBI
  • BioCyc: SA_RS03735 BioCyc
  • MicrobesOnline: see SA0655

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGAGTAACAGAGTTATTAACAAAAGATACGATAGCAATGGATTTAATGGCAAATGAC
    AAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGAT
    GTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGC
    GAAGGTATTGCTATTCCACATGCGAAAGTAGCCGCAGTTAAGTCTCCAGCTATCGCATTC
    GGTAAATCTAAAGAAGGTGTAGATTACCAAAGTTTAGATATGCAACCAGCTCATTTATTC
    TTTATGATTGCAGCGCCAGAAGGTGGCGCCCAAACACATCTAGATGCTTTAGCTAAGTTG
    TCTGGTATTTTAATGGATGAAAATGTACGCGAGAAATTATTACATGCTTCATCACCTGAA
    GAAGTACTAGCGATTATAGATGAGGCTGATGATGAAGTGACAAAAGAAGAAGAGGCAGAA
    GCTGAAGCACAACAAGTTGCAACTGCAGAACAATCATCTAAACAATCTAACGAGCCATAT
    GTGTTAGCAGTAACTGCTTGTCCAACAGGTATTGCACATACGTACATGGCACGTGACGCA
    TTGAAAAAACAAGCGGATAAGATGGGTATTAAAATTAAAGTAGAAACGAACGGTTCAAGC
    GGCATTAAAAACCATTTAACTGAACAAGATATTGAAAATGCAACAGGTATCATTGTTGCT
    GCTGATGTTCATGTTGAGACGGATCGCTTCGATGGTAAAAATGTCGTAGAAGTACCAGTA
    GCAGATGGTATTAAACGCCCAGAAGAATTAATTAATAAAGCATTAGATACAAGTCGTAAA
    CCTTTTGTTGCCCGTGATGGTCAAAGAAAAGGTAACTCAAATGACAGTCAAGAAAAATTA
    AGCCCAGGTAAAGCATTCTATAAACACTTAATGAACGGTGTTTCTAACATGTTGCCACTT
    GTAATATCTGGTGGTATTTTAATGGCAATTGTATTTTTATTTGGAGCAAATTCATTTAAT
    CCAAAAAGCTCAGAGTACAATGCGTTTGCAGAGCAGCTTTGGAACATTGGTAGTAAAAGT
    GCATTCGCGCTAATCATTCCAATTTTATCTGGATTCATTGCACGTAGTATTGCGGATAAA
    CCTGGTTTCGCTTCAGGTCTTGTAGGTGGTATGTTAGCAATTTCAGGTGGTTCAGGATTT
    ATTGGTGGTATTATTGCAGGTTTCTTAGCAGGTTACTTAACACAAGGTGTTAAAGCCATG
    ACACGTAAGTTACCACAAGCATTAGAGGGATTAAAGCCAACATTAATTTATCCACTATTA
    ACAGTGACAGCTACAGGCTTATTGATGATTTATGTCTTTAATCCACCAGCATCTTGGTTA
    AATCATTTGTTATTAGATGGATTAAACAATTTATCAGGTTCTAATATTGTATTATTAGGT
    TTAGTTATTGGCGCTATGATGGCGATTGATATGGGCGGTCCATTCAACAAAGCGGCATAT
    GTTTTTGCAACAGGTGCGTTGATTGAAGGTAATGCAGCACCAATTACAGCTGCAATGATT
    GGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTCAGACGTAAATTT
    ACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCCTTTATTACA
    GAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATT
    GGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCTCGAATTACTGCGCCACAT
    GGTGGTATTATTGTAATTGTTGGTACTGATGGTGCACACTTACTTCAAACTCTTATTGCA
    CTTCTAGTTGGTACATTAGTTTCAGCATTAATTTATGGACTTATTAAACCAAAGTTAACT
    GAAACAGAAATCGAAGCTTCAAAATCAATGGACGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1959

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03735 [old locus tag: SA0655 ]
  • symbol: SA_RS03735
  • description: PTS fructose transporter subunit IIC
  • length: 652
  • theoretical pI: 5.66057
  • theoretical MW: 68799.3
  • GRAVY: 0.19954

⊟Function[edit | edit source]

  • TIGRFAM:
    PTS system, Fru family, IIC component (TIGR01427; HMM-score: 396.6)
    and 7 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 130.9)
    Signal transduction Signal transduction PTS PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 130.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, Fru family, IIB component (TIGR00829; EC 2.7.1.69; HMM-score: 110.4)
    Signal transduction Signal transduction PTS PTS system, Fru family, IIB component (TIGR00829; EC 2.7.1.69; HMM-score: 110.4)
    Signal transduction Signal transduction PTS PTS IIA-like nitrogen-regulatory protein PtsN (TIGR01419; HMM-score: 79.1)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 29)
    Cellular processes Cellular processes Biosynthesis of natural products nif11-like leader peptide domain (TIGR03798; HMM-score: 12.7)
  • TheSEED: see SA0655
  • PFAM:
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 141.6)
    and 5 more
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 64.3)
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 62.2)
    no clan defined Nif11; Nif11 domain (PF07862; HMM-score: 14.6)
    LysE (CL0292) UPF0016; Uncharacterized protein family UPF0016 (PF01169; HMM-score: 10)
    no clan defined DUF1453; Protein of unknown function (DUF1453) (PF07301; HMM-score: 6.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008726
    • TAT(Tat/SPI): 0.001602
    • LIPO(Sec/SPII): 0.000691
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASFKEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKEGVDYQSLDMQPAHLFFMIAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAEAEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNGSSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALDTSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLFGANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGMLAISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMIYVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALIEGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPFAAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVGTLVSALIYGLIKPKLTETEIEASKSMDE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA, FruR* see SA0655

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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