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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04495 [old locus tag: SA0787 ]
  • pan locus tag?: SAUPAN003025000
  • symbol: SA_RS04495
  • pan gene symbol?: tnp
  • synonym:
  • product: ISL3 family transposase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04495 [old locus tag: SA0787 ]
  • symbol: SA_RS04495
  • product: ISL3 family transposase
  • replicon: chromosome
  • strand: +
  • coordinates: 893906..895225
  • length: 1320
  • essential: no DEG

Accession numbers[edit | edit source]

  • Location: NC_002745 (893906..895225) NCBI
  • BioCyc: SA_RS04495 BioCyc
  • MicrobesOnline: see SA0787

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGTAATGATACCTTAGAATTACTAAGAATAAAAGATGAAAATATAAAATATATAAAC
    CAAGAAATTGACGTCATTATCAAAGGAAAAAAAGCAACAGTGGTTAATGCTGTACTAACG
    TATAAGCCTTCGGCCTGTTATTGTTGTGGAGTTAAAAATGAAGGACAAATTCATAAACAT
    GGTAAGCGTGTTTCTCGTATTACTTTACTTAAAACTCAAGGGTATAACACATACCTCAAC
    TTAGCTAAACAACGTTTTAAATGCTTAGAATGCAATGGCACTTTTACTGCTAAAACGTCA
    ATTGTTGATGAGTCGTGTTTTATCTCAAGATGTGTTACTCAAAAAGTTATAGAAGAAGCT
    ACTAAAGTTAAAACAGAGATTGATACTGCAGAAGATAACTGTATCTCTCCATCTACTGTA
    AGTCGTATTAGAACTAAAGCGGCTAATTCATTACGAATTAAACCGTTTAATTGTTTGCCA
    GAACACATCGCTATGGATGAATTTAAAAGCGTTAAAAATGTAACTGGATCAATGAGTTTC
    ATTTTTATAGATAATGATACTCATGATGTTATAGATATTTTAGAAAATAGAACTACAAGA
    TTCTTGCGTGCCTATTTCGAGCGATTCGATTTAAAAAATCGACAACAAGTTAAGACGGTT
    ACTATTGACATGTATGAACCCTATGTCCGATTATTTCGCGACCTATTTCCTAATGCAGCT
    ATTATTTTTGACAGATTCCATATCGTTCAACATTTAAATAGGGAACTTAATAAGTATCGT
    GTACAAGTTATGAATGAATACCGTAATAAAAAAGGACCTAATTATACAATTTTTAAGAAT
    AACTGGAAAGTTCTATTGATGGATACTAGTAAAACCATATTTAGTAAATACAGATGGAAT
    AAATCTTTTAAGGCTTATAAACGCTCATCTGACATTGTAGAATTCATGCTTTCAAAAGAC
    GATATACTACGACGCTCCTACGAACTTGTCCAAGGATTAAGAAAAGATCTAAGGTTATGT
    AATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAAGGT
    GTATGGAAAGTGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTAT
    TACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAG
    CGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTC
    AGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAA
    60
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    1020
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    1320

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS04495 [old locus tag: SA0787 ]
  • symbol: SA_RS04495
  • description: ISL3 family transposase
  • length: 439
  • theoretical pI: 10.4384
  • theoretical MW: 51570.7
  • GRAVY: -0.48656

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.5)
    Metabolism Central intermediary metabolism One-carbon metabolism S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.5)
  • TheSEED: see SA0787
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 174.2)
    and 4 more
    Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 42.4)
    HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.6)
    no clan defined DUF3795; Protein of unknown function (DUF3795) (PF12675; HMM-score: 10.3)
    DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 9.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.179974
    • TAT(Tat/SPI): 0.017784
    • LIPO(Sec/SPII): 0.012804
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPSACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIVDESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLPEHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVKTVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPNYTIFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRRSYELVQGLRKDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKKKTTKPNNGLAA

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]