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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04705 [old locus tag: SA0828 ]
  • pan locus tag?: SAUPAN003077000
  • symbol: SA_RS04705
  • pan gene symbol?: addA
  • synonym:
  • product: ATP-dependent helicase/nuclease subunit A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04705 [old locus tag: SA0828 ]
  • symbol: SA_RS04705
  • product: ATP-dependent helicase/nuclease subunit A
  • replicon: chromosome
  • strand: +
  • coordinates: 935854..939507
  • length: 3654
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (935854..939507) NCBI
  • BioCyc: SA_RS04705 BioCyc
  • MicrobesOnline: see SA0828

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
    ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATT
    TACGCAACTGGACAAGATGTACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTA
    CTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCATTGATGTCGATCGACTTTTA
    GTCGTAACGTTTACAAACTTAAGCGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
    CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATT
    CATCAAGCACAAATATCTACACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTAT
    GATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTGAAGCTGAAAATATTTTATTA
    TTAGAACAAACGATAGATGAGGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
    ATTGAATTAACAGAACAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATT
    AAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTG
    GTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACTAACAGACTTA
    TCTAAAGTATTTATCACAGCTGCCTATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
    ATGATGGATGGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGG
    CGTGTTTTAGAAGGTGGTTTTATTGATATACCTTATTTAACTGATCACGAATTTGGCGCG
    CGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATGAAATGATGGTCGATGCCTTA
    GAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAAAATGAT
    TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTA
    AAGTACCTTGCGCGTATTGTGAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGC
    AAAAATATTCTGGATTTTTCTGATTATGAACATTTTGCATTACAAATTTTAACTAATGAG
    GATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTTCAAGAAATATTGGTC
    GATGAGTATCAAGATACGAACCGGGTTCAAGAGAAAATACTATCTTGCATCAAAACGGGT
    GATGAACATAATGGTAATTTATTTATGGTTGGAGATGTTAAGCAATCCATTTATAAATTT
    AGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAACGCTTTACTATAGATGGAGAT
    GGCACTGGACGTCGAATTGATTTGTCGCAAAACTTCCGTTCTCGAAAAGAAGTACTGTCA
    ACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGAT
    GAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTA
    AAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCG
    CATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAA
    ACAGGAAGCTATAGAAGTGCGACATACAAAGATATCGTTATTCTAGAACGCAGCTTTGGA
    CAAGCTCGCAATTTACAACAAGCCTTTAAAAATGAAGATATTCCATTCCATGTGAATAGT
    CGTGAAGGTTACTTTGAACAAACAGAAGTCCGCTTAGTATTATCATTTTTAAGAGCGATA
    GATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGCTCCGTTATATATCAGTTC
    AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAA
    TCGATTGTAAATTACATTAATGACGAAGCAGCAGATGCTATTTTAGTTGATAAATTAAAA
    ATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTAAAGATCATCCGGTGTATCAG
    TTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
    GGACGTGGACGACGTGCAAACCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAAT
    TCAAGTTTTAGAGGTTTATATCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGC
    AAAGATTTTGGTGAGGAAAATGTAGTTGGTCCAAACGATAATGTTGTTAGAATGATGACA
    ATTCATAGTAGTAAAGGTCTAGAGTTTCCATTTGTCATTTATTCTGGATTGTCAAAAGAT
    TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATG
    GATTATTTTGATGTGGATAAAGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAAA
    GCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGATTAGTCTATGTAGCATTAACA
    AGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCGTTACTAGAA
    CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCA
    AATCCGTTCCATCTTATTTATAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGAT
    GATTTAAAATTTGAAAAAGATATAGCACAAGTTGAAGATAGTAGTCGTCCGAATGTAAAT
    ATTTCAATTATATACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATAATGAATAT
    CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAGGATGTTAAAGCACAAATT
    AAACACCAACTTGATTATCAATATCCATATGTAAATGATACTAAAAAGCCATCAAAACAA
    TCTGTTTCTGAATTGAAAAGGCAATATGAAACAGAAGAAAGTGGCACAAGTTACGAACGA
    GTAAGACAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
    GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCA
    TTCAAAAAAGAACGCATATCTGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGAT
    AAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTATGGATGAAATAATGACATTT
    ATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
    TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGTCTCAATT
    ATTCAAGGTATGATTGACTTAATCTTTGTTAAAGATGGTGTGCATTATTTTGTAGACTAT
    AAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATGAAGAAATTGGTACACAATTA
    AAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATACTTAAT
    AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG
    60
    120
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    360
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    2160
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    2820
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    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3654

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS04705 [old locus tag: SA0828 ]
  • symbol: SA_RS04705
  • description: ATP-dependent helicase/nuclease subunit A
  • length: 1217
  • theoretical pI: 4.92652
  • theoretical MW: 141258
  • GRAVY: -0.471159

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair helicase-exonuclease AddAB, AddA subunit (TIGR02785; EC 3.1.-.-,3.6.4.12; HMM-score: 1457.2)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair double-strand break repair helicase AddA (TIGR02784; HMM-score: 214.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair exodeoxyribonuclease V, beta subunit (TIGR00609; EC 3.1.11.5; HMM-score: 214.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 210.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA helicase II (TIGR01075; EC 3.6.4.12; HMM-score: 195.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 191)
  • TheSEED: see SA0828
  • PFAM:
    P-loop_NTPase (CL0023) UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 271)
    and 10 more
    UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 112.3)
    AAA_19; AAA domain (PF13245; HMM-score: 75.5)
    UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 38)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 28.7)
    PDDEXK (CL0236) PDDEXK_1; PD-(D/E)XK nuclease superfamily (PF12705; HMM-score: 25.8)
    P-loop_NTPase (CL0023) Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 22.2)
    AAA_12; AAA domain (PF13087; HMM-score: 19.5)
    no clan defined Occludin_ELL; Occludin homology domain (PF07303; HMM-score: 16.4)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.2)
    no clan defined DUF2564; Protein of unknown function (DUF2564) (PF10819; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008816
    • TAT(Tat/SPI): 0.000928
    • LIPO(Sec/SPII): 0.001407
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAFIELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTDHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKNDYFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVKGYLYFFKFGTLQL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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