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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06045 [old locus tag: SA1066 ]
  • pan locus tag?: SAUPAN003506000
  • symbol: SA_RS06045
  • pan gene symbol?: thiN
  • synonym:
  • product: thiamine pyrophosphokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06045 [old locus tag: SA1066 ]
  • symbol: SA_RS06045
  • product: thiamine pyrophosphokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1206133..1206774
  • length: 642
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1206133..1206774) NCBI
  • BioCyc: SA_RS06045 BioCyc
  • MicrobesOnline: see SA1066

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCATATAAATTTATTATGTTCTAATCGACACTTGCCGCAAGATATTTGGGCCAAAAGT
    AATGAAGGTAAATGGGGCGGCGTTGATAGAGGTGCTTTGATTTTATTGAAGCATCAAATT
    ATCCCTTTTTTCTCAGTGGGAGACTTTGATTCAGTCAGTAAAGAAGAACGCCAACTTCTA
    ACAGAACAGTTACAAATCAAACCAGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTA
    GCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGC
    GGACGATTAGATCACTTTTTTGGGGCAATTCAGTTATTATTGAAAAAAGCATATTATAAA
    CATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAATTGAATTATTGCCTAAAGGT
    CAACATACAGTTGAAAAAGATAAGAGTTATCCGTACATTTCATTTATACCGATGACTGAT
    GATGTAGAACTTTCTCTAGCAGGTTTTAAATATAATTTAGCTAGACAAATGCTTAATATA
    GGTTCTACTTTAACTATTTCAAATGAAATTGAGTCTTTGCAAGCGAAAGTAACTGTACAT
    GATGGGTTGATTTTGCAAATTAGAAGTACAGATTTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    642

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS06045 [old locus tag: SA1066 ]
  • symbol: SA_RS06045
  • description: thiamine pyrophosphokinase
  • length: 213
  • theoretical pI: 6.30565
  • theoretical MW: 23883.2
  • GRAVY: -0.14507

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine pyrophosphokinase (TIGR01378; EC 2.7.6.2; HMM-score: 181.2)
  • TheSEED: see SA1066
  • PFAM:
    no clan defined TPK_catalytic; Thiamin pyrophosphokinase, catalytic domain (PF04263; HMM-score: 64.6)
    TPK_B1_binding; Thiamin pyrophosphokinase, vitamin B1 binding domain (PF04265; HMM-score: 53.5)
    and 1 more
    Tox-SHH; HNH/Endo VII superfamily toxin with a SHH signature (PF15652; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003207
    • TAT(Tat/SPI): 0.00024
    • LIPO(Sec/SPII): 0.000836
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHINLLCSNRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFFSVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGRLDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTDDVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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