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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06270 [old locus tag: SA1107 ]
  • pan locus tag?: SAUPAN003565000
  • symbol: polC
  • pan gene symbol?: polC
  • synonym:
  • product: DNA polymerase III subunit alpha

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06270 [old locus tag: SA1107 ]
  • symbol: polC
  • product: DNA polymerase III subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1253878..1258188
  • length: 4311
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1253878..1258188) NCBI
  • BioCyc: SA_RS06270 BioCyc
  • MicrobesOnline: see SA1107

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTA
    GATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGA
    ACATGGGAATTTCATATTACATTACCACAATTCTTAGCTCATGAAGATTATTTATTATTT
    ATAAATGCAATAGAGCAAGAGTTTAAAGATATCGCCAACGTTACATGTCGTTTTACGGTA
    ACAAATGGCACGAATCAAGATGAACATGCAATTAAATACTTTGGGCACTGTATTGACCAA
    ACAGCTTTATCTCCAAAAGTTAAAGGTCAATTGAAACAGAAAAAGCTTATTATGTCTGGA
    AAAGTATTAAAAGTAATGGTATCAAATGACATTGAACGTAATCATTTTGATAAGGCATGT
    AATGGAAGTCTTATCAAAGCGTTTAGAAATTGTGGTTTTGATATCGATAAAATCATATTC
    GAAACAAATGATAATGATCAAGAACAAAACTTAGCTTCTTTAGAAGCACATATTCAAGAA
    GAAGACGAACAAAGTGCACGATTGGCAACAGAGAAACTTGAAAAAATGAAAGCTGAAAAA
    GCGAAACAACAAGATAACAACGAAAGTGCTGTCGATAAGTGTCAAATTGGTAAGCCGATT
    CAAATTGAAAATATTAAACCAATTGAATCTATTATTGAGGAAGAGTTTAAAGTTGCAATA
    GAGGGTGTCATTTTTGATATAAACTTAAAAGAACTTAAAAGTGGTCGTCATATCGTAGAA
    ATTAAAGTGACTGACTATACGGACTCATTAGTTTTAAAAATGTTTACTCGTAAAAACAAA
    GATGATTTAGAACATTTTAAAGCGCTAAGTGTAGGTAAATGGGTTAGGGCTCAAGGTCGT
    ATTGAAGAAGATACATTTATTAGAGATTTAGTTATGATGATGTCTGATATTGAAGAGATT
    AAAAAAGCGACAAAAAAAGATAAGGCTGAAGAAAAGCGTGTAGAATTCCACTTGCATACT
    GCAATGAGCCAAATGGATGGTATACCCAATATTGGTGCGTATGTTAAACAGGCAGCAGAC
    TGGGGACATCCAGCCATTGCGGTTACAGACCATAATGTTGTGCAAGCATTTCCAGATGCT
    CACGCAGCAGCGGAAAAACATGGCATTAAAATGATATACGGTATGGAAGGTATGTTAGTT
    GATGATGGTGTTCCGATTGCATACAAACCACAAGATGTCGTATTAAAAGATGCTACTTAT
    GTTGTGTTCGACGTTGAGACAACTGGTTTATCAAATCAGTATGATAAAATCATCGAGCTT
    GCAGCTGTGAAAGTTCATAACGGTGAAATCATCGATAAGTTTGAAAGGTTTAGTAATCCG
    CATGAACGATTATCGGAAACGATTATCAATTTGACGCATATTACTGATGATATGTTAGTA
    GATGCCCCTGAGATTGAAGAAGTACTTACAGAGTTTAAAGAATGGGTTGGCGATGCGATA
    TTCGTAGCGCATAATGCTTCGTTTGATATGGGCTTTATCGATACGGGATATGAACGTCTT
    GGGTTTGGACCATCAACGAATGGTGTTATCGATACTTTAGAATTATCTCGTACGATTAAT
    ACTGAATATGGTAAACATGGTTTGAATTTCTTAGCTAAAAAATATGGCGTAGAATTAACG
    CAACATCACCGTGCCATTTATGATACAGAAGCAACAGCTTACATTTTCATAAAAATGGTT
    CAACAAATGAAAGAATTAGGTGTATTAAATCATAACGAAATCAACAAAAAACTCAGTAAT
    GAAGATGCATATAAACGTGCAAGACCTAGCCATGTCACATTAATTGTACAAAACCAACAA
    GGTCTTAAAAATCTATTTAAAATTGTAAGTGCATCATTGGTGAAGTATTTCTACCGTACA
    CCTCGAATTCCACGTTCATTGTTAGATGAATATCGTGAGGGATTATTGGTAGGTACAGCG
    TGTGATGAAGGTGAATTATTTACGGCAGTTATGCAGAAGGACCAGAGCCAAGTTGAAAAA
    ATTGCCAAATATTATGATTTTATTGAAATTCAACCACCGGCACTTTATCAAGATTTAATT
    GATAGAGAGCTTATTAGAGATACTGAAACATTACATGAAATTTATCAACGTTTAATACAT
    GCAGGTGACACAGCGGGTATACCTGTTATTGCGACAGGAAATGCACACTATTTGTTTGAA
    CATGATGGTATCGCACGTAAAATTTTAATAGCATCACAACCCGGCAATCCACTTAATCGC
    TCAACTTTACCGGAAGCACATTTTAGAACTACAGATGAAATGTTAAACGAGTTTCATTTT
    TTAGGTGAAGAAAAAGCGCATGAAATTGTTGTGAAAAATACAAACGAATTAGCAGATCGA
    ATTGAACGTGTTGTTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAAC
    GAAGAAATTAGAGAACTAAGTTATACAAATGCGCGTAAACTGTATGGTGAAGACCTGCCT
    CAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCG
    GTAATTTACTTAATTTCGCAACGTTTAGTTAAAAAATCATTAGATGATGGATACTTAGTT
    GGTTCCCGTGGTTCAGTAGGTTCTAGTTTTGTAGCGACAATGACTGAGATTACTGAAGTA
    AACCCGTTACCGCCACACTATATTTGTCCGAACTGTAAAACGAGTGAATTTTTCAATGAT
    GGTTCAGTAGGATCAGGATTCGATTTACCTGATAAGACGTGTGAAACTTGTGGAGCGCCA
    CTTATTAAAGAAGGACAAGATATTCCGTTTGAAACATTTTTAGGATTTAAGGGAGATAAA
    GTTCCTGATATCGACTTAAACTTTAGTGGTGAATATCAACCGAATGCCCATAACTACACA
    AAAGTATTATTTGGTGAGGATAAAGTATTCCGTGCAGGTACAATTGGTACCGTTGCTGAA
    AAGACTGCTTTTGGTTATGTTAAAGGTTATTTGAATGATCAAGGCATCCACAAAAGAGGC
    GCTGAAATAGATCGACTCGTTAAAGGATGTACAGGTGTTAAACGTACAACTGGACAGCAT
    CCAGGAGGTATTATTGTAGTACCTGATTACATGGATATTTATGATTTTACGCCGATACAA
    TATCCTGCCGATGATCAAAATTCAGCATGGATGACGACACATTTTGATTTCCATTCTATT
    CATGATAATGTATTAAAACTTGATATACTTGGACACGATGATCCAACAATGATTCGTATG
    CTTCAAGATTTATCGGGCATTGATCCAAAAACGATACCTGTAGATGACAAAGAAGTCATG
    CAAATATTTAGTACACCTGAAAGTTTAGGTGTTACTGAAGATGAAATTTTATGTAAAACA
    GGTACGTTTGGGGTTCCAGAATTCGGTACAGGGTTCGTGCGTCAAATGTTAGAAGATACA
    AAGCCAACAACATTTTCTGAATTAGTTCAAATCTCAGGATTATCTCATGGTACAGATGTG
    TGGTTAGGCAATGCTCAAGAATTAATTAAAACCGGTATATGTGATTTATCAAGTGTAATT
    GGTTGTCGTGATGATATCATGGTTTATTTAATGTATGCTGGTTTAGAACCATCAATGGCT
    TTTAAAATAATGGAGTCAGTACGTAAAGGTAAAGGTTTAACTGAAGAAATGATTGAAACG
    ATGAAAGAAAATGAAGTGCCGGATTGGTATTTAGATTCATGTCTTAAAATTAAGTACATG
    TTCCCTAAAGCCCATGCAGCAGCATACGTTTTAATGGCAGTACGTATCGCATATTTCAAA
    GTACATCATCCACTTTATTACTATGCATCTTACTTTACAATTCGTGCGTCAGATTTTGAT
    TTAATCACGATGATTAAAGATAAAACAAGCATTCGAAATACTGTAAAAGACATGTATTCT
    CGCTATATGGATCTAGGTAAAAAAGAAAAAGACGTATTAACAGTCTTGGAAATTATGAAT
    GAAATGGCGCATCGAGGTTATCGAATGCAACCGATTAGTTTAGAAAAGAGTCAGGCGTTC
    GAATTTATCATTGAAGGCGATACACTTATTCCGCCGTTCATATCAGTGCCTGGGCTTGGC
    GAAAACGTTGCGAAACGAATTGTTGAAGCTCGTGACGATGGCCCATTTTTATCAAAAGAA
    GATTTAAACAAAAAAGCTGGATTATCTCAGAAAATTATTGAGTATTTAGATGAGTTAGGC
    TCATTACCGAATTTACCAGATAAAGCTCAACTTTCGATATTTGATATGTAA
    60
    120
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    2160
    2220
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    2460
    2520
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    2640
    2700
    2760
    2820
    2880
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    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4311

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS06270 [old locus tag: SA1107 ]
  • symbol: PolC
  • description: DNA polymerase III subunit alpha
  • length: 1436
  • theoretical pI: 5.0377
  • theoretical MW: 162489
  • GRAVY: -0.327646

Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 2033.2)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 217.9)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 184.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 149.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 94.7)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 45.4)
  • TheSEED: see SA1107
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha subunit (PF07733; HMM-score: 331.7)
    and 11 more
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 182.2)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 149.3)
    DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 63.1)
    no clan defined DNA_pol3_a_NI; DNA polymerase III polC-type N-terminus I (PF14480; HMM-score: 59.8)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 37.9)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 36.4)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.6)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 15.4)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 11.2)
    RNase_H (CL0219) CAF1; CAF1 family ribonuclease (PF04857; HMM-score: 5.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000845
    • TAT(Tat/SPI): 0.000099
    • LIPO(Sec/SPII): 0.000185
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEFHITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTALSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIFETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGKPIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFTRKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMIYGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEIIDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASFDMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAIYDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKNLFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIAKYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFEHDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELADRIERVVPIKDELYTPRMEGANEEIRELSYTNARKLYGEDLPQIVIDRLEKELKSIIGNGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKTSEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEYQPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGCTGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLDILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVPEFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRDDIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFPKAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYSRYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPGLGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

Relevant publications[edit | edit source]