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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06270 [old locus tag: SA1107 ]
  • pan locus tag?: SAUPAN003565000
  • symbol: polC
  • pan gene symbol?: polC
  • synonym:
  • product: DNA polymerase III subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06270 [old locus tag: SA1107 ]
  • symbol: polC
  • product: DNA polymerase III subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1253878..1258188
  • length: 4311
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1253878..1258188) NCBI
  • BioCyc: SA_RS06270 BioCyc
  • MicrobesOnline: see SA1107

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    481
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    601
    661
    721
    781
    841
    901
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    1021
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    1321
    1381
    1441
    1501
    1561
    1621
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    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    4261
    ATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTA
    GATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGA
    ACATGGGAATTTCATATTACATTACCACAATTCTTAGCTCATGAAGATTATTTATTATTT
    ATAAATGCAATAGAGCAAGAGTTTAAAGATATCGCCAACGTTACATGTCGTTTTACGGTA
    ACAAATGGCACGAATCAAGATGAACATGCAATTAAATACTTTGGGCACTGTATTGACCAA
    ACAGCTTTATCTCCAAAAGTTAAAGGTCAATTGAAACAGAAAAAGCTTATTATGTCTGGA
    AAAGTATTAAAAGTAATGGTATCAAATGACATTGAACGTAATCATTTTGATAAGGCATGT
    AATGGAAGTCTTATCAAAGCGTTTAGAAATTGTGGTTTTGATATCGATAAAATCATATTC
    GAAACAAATGATAATGATCAAGAACAAAACTTAGCTTCTTTAGAAGCACATATTCAAGAA
    GAAGACGAACAAAGTGCACGATTGGCAACAGAGAAACTTGAAAAAATGAAAGCTGAAAAA
    GCGAAACAACAAGATAACAACGAAAGTGCTGTCGATAAGTGTCAAATTGGTAAGCCGATT
    CAAATTGAAAATATTAAACCAATTGAATCTATTATTGAGGAAGAGTTTAAAGTTGCAATA
    GAGGGTGTCATTTTTGATATAAACTTAAAAGAACTTAAAAGTGGTCGTCATATCGTAGAA
    ATTAAAGTGACTGACTATACGGACTCATTAGTTTTAAAAATGTTTACTCGTAAAAACAAA
    GATGATTTAGAACATTTTAAAGCGCTAAGTGTAGGTAAATGGGTTAGGGCTCAAGGTCGT
    ATTGAAGAAGATACATTTATTAGAGATTTAGTTATGATGATGTCTGATATTGAAGAGATT
    AAAAAAGCGACAAAAAAAGATAAGGCTGAAGAAAAGCGTGTAGAATTCCACTTGCATACT
    GCAATGAGCCAAATGGATGGTATACCCAATATTGGTGCGTATGTTAAACAGGCAGCAGAC
    TGGGGACATCCAGCCATTGCGGTTACAGACCATAATGTTGTGCAAGCATTTCCAGATGCT
    CACGCAGCAGCGGAAAAACATGGCATTAAAATGATATACGGTATGGAAGGTATGTTAGTT
    GATGATGGTGTTCCGATTGCATACAAACCACAAGATGTCGTATTAAAAGATGCTACTTAT
    GTTGTGTTCGACGTTGAGACAACTGGTTTATCAAATCAGTATGATAAAATCATCGAGCTT
    GCAGCTGTGAAAGTTCATAACGGTGAAATCATCGATAAGTTTGAAAGGTTTAGTAATCCG
    CATGAACGATTATCGGAAACGATTATCAATTTGACGCATATTACTGATGATATGTTAGTA
    GATGCCCCTGAGATTGAAGAAGTACTTACAGAGTTTAAAGAATGGGTTGGCGATGCGATA
    TTCGTAGCGCATAATGCTTCGTTTGATATGGGCTTTATCGATACGGGATATGAACGTCTT
    GGGTTTGGACCATCAACGAATGGTGTTATCGATACTTTAGAATTATCTCGTACGATTAAT
    ACTGAATATGGTAAACATGGTTTGAATTTCTTAGCTAAAAAATATGGCGTAGAATTAACG
    CAACATCACCGTGCCATTTATGATACAGAAGCAACAGCTTACATTTTCATAAAAATGGTT
    CAACAAATGAAAGAATTAGGTGTATTAAATCATAACGAAATCAACAAAAAACTCAGTAAT
    GAAGATGCATATAAACGTGCAAGACCTAGCCATGTCACATTAATTGTACAAAACCAACAA
    GGTCTTAAAAATCTATTTAAAATTGTAAGTGCATCATTGGTGAAGTATTTCTACCGTACA
    CCTCGAATTCCACGTTCATTGTTAGATGAATATCGTGAGGGATTATTGGTAGGTACAGCG
    TGTGATGAAGGTGAATTATTTACGGCAGTTATGCAGAAGGACCAGAGCCAAGTTGAAAAA
    ATTGCCAAATATTATGATTTTATTGAAATTCAACCACCGGCACTTTATCAAGATTTAATT
    GATAGAGAGCTTATTAGAGATACTGAAACATTACATGAAATTTATCAACGTTTAATACAT
    GCAGGTGACACAGCGGGTATACCTGTTATTGCGACAGGAAATGCACACTATTTGTTTGAA
    CATGATGGTATCGCACGTAAAATTTTAATAGCATCACAACCCGGCAATCCACTTAATCGC
    TCAACTTTACCGGAAGCACATTTTAGAACTACAGATGAAATGTTAAACGAGTTTCATTTT
    TTAGGTGAAGAAAAAGCGCATGAAATTGTTGTGAAAAATACAAACGAATTAGCAGATCGA
    ATTGAACGTGTTGTTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAAC
    GAAGAAATTAGAGAACTAAGTTATACAAATGCGCGTAAACTGTATGGTGAAGACCTGCCT
    CAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCG
    GTAATTTACTTAATTTCGCAACGTTTAGTTAAAAAATCATTAGATGATGGATACTTAGTT
    GGTTCCCGTGGTTCAGTAGGTTCTAGTTTTGTAGCGACAATGACTGAGATTACTGAAGTA
    AACCCGTTACCGCCACACTATATTTGTCCGAACTGTAAAACGAGTGAATTTTTCAATGAT
    GGTTCAGTAGGATCAGGATTCGATTTACCTGATAAGACGTGTGAAACTTGTGGAGCGCCA
    CTTATTAAAGAAGGACAAGATATTCCGTTTGAAACATTTTTAGGATTTAAGGGAGATAAA
    GTTCCTGATATCGACTTAAACTTTAGTGGTGAATATCAACCGAATGCCCATAACTACACA
    AAAGTATTATTTGGTGAGGATAAAGTATTCCGTGCAGGTACAATTGGTACCGTTGCTGAA
    AAGACTGCTTTTGGTTATGTTAAAGGTTATTTGAATGATCAAGGCATCCACAAAAGAGGC
    GCTGAAATAGATCGACTCGTTAAAGGATGTACAGGTGTTAAACGTACAACTGGACAGCAT
    CCAGGAGGTATTATTGTAGTACCTGATTACATGGATATTTATGATTTTACGCCGATACAA
    TATCCTGCCGATGATCAAAATTCAGCATGGATGACGACACATTTTGATTTCCATTCTATT
    CATGATAATGTATTAAAACTTGATATACTTGGACACGATGATCCAACAATGATTCGTATG
    CTTCAAGATTTATCGGGCATTGATCCAAAAACGATACCTGTAGATGACAAAGAAGTCATG
    CAAATATTTAGTACACCTGAAAGTTTAGGTGTTACTGAAGATGAAATTTTATGTAAAACA
    GGTACGTTTGGGGTTCCAGAATTCGGTACAGGGTTCGTGCGTCAAATGTTAGAAGATACA
    AAGCCAACAACATTTTCTGAATTAGTTCAAATCTCAGGATTATCTCATGGTACAGATGTG
    TGGTTAGGCAATGCTCAAGAATTAATTAAAACCGGTATATGTGATTTATCAAGTGTAATT
    GGTTGTCGTGATGATATCATGGTTTATTTAATGTATGCTGGTTTAGAACCATCAATGGCT
    TTTAAAATAATGGAGTCAGTACGTAAAGGTAAAGGTTTAACTGAAGAAATGATTGAAACG
    ATGAAAGAAAATGAAGTGCCGGATTGGTATTTAGATTCATGTCTTAAAATTAAGTACATG
    TTCCCTAAAGCCCATGCAGCAGCATACGTTTTAATGGCAGTACGTATCGCATATTTCAAA
    GTACATCATCCACTTTATTACTATGCATCTTACTTTACAATTCGTGCGTCAGATTTTGAT
    TTAATCACGATGATTAAAGATAAAACAAGCATTCGAAATACTGTAAAAGACATGTATTCT
    CGCTATATGGATCTAGGTAAAAAAGAAAAAGACGTATTAACAGTCTTGGAAATTATGAAT
    GAAATGGCGCATCGAGGTTATCGAATGCAACCGATTAGTTTAGAAAAGAGTCAGGCGTTC
    GAATTTATCATTGAAGGCGATACACTTATTCCGCCGTTCATATCAGTGCCTGGGCTTGGC
    GAAAACGTTGCGAAACGAATTGTTGAAGCTCGTGACGATGGCCCATTTTTATCAAAAGAA
    GATTTAAACAAAAAAGCTGGATTATCTCAGAAAATTATTGAGTATTTAGATGAGTTAGGC
    TCATTACCGAATTTACCAGATAAAGCTCAACTTTCGATATTTGATATGTAA
    60
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    2160
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    3240
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    3420
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    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
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    4140
    4200
    4260
    4311

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS06270 [old locus tag: SA1107 ]
  • symbol: PolC
  • description: DNA polymerase III subunit alpha
  • length: 1436
  • theoretical pI: 5.0377
  • theoretical MW: 162489
  • GRAVY: -0.327646

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 2033.2)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 217.9)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 184.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 149.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 94.7)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 45.4)
  • TheSEED: see SA1107
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha subunit (PF07733; HMM-score: 331.7)
    and 11 more
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 182.2)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 149.3)
    DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 63.1)
    no clan defined DNA_pol3_a_NI; DNA polymerase III polC-type N-terminus I (PF14480; HMM-score: 59.8)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 37.9)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 36.4)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.6)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 15.4)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 11.2)
    RNase_H (CL0219) CAF1; CAF1 family ribonuclease (PF04857; HMM-score: 5.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000845
    • TAT(Tat/SPI): 0.000099
    • LIPO(Sec/SPII): 0.000185
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEFHITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTALSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIFETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGKPIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFTRKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMIYGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEIIDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASFDMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAIYDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKNLFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIAKYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFEHDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELADRIERVVPIKDELYTPRMEGANEEIRELSYTNARKLYGEDLPQIVIDRLEKELKSIIGNGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKTSEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEYQPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGCTGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLDILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVPEFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRDDIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFPKAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYSRYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPGLGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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