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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06395 [old locus tag: SA1131 ]
  • pan locus tag?: SAUPAN003592000
  • symbol: SA_RS06395
  • pan gene symbol?: —
  • synonym:
  • product: 2-oxoglutarate ferredoxin oxidoreductase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06395 [old locus tag: SA1131 ]
  • symbol: SA_RS06395
  • product: 2-oxoglutarate ferredoxin oxidoreductase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1285149..1286909
  • length: 1761
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1285149..1286909) NCBI
  • BioCyc: SA_RS06395 BioCyc
  • MicrobesOnline: see SA1131

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCACAATTATCGTGGAAAGTTGGCGGTCAACAAGGCGAAGGTATTGAATCAACT
    GGGGAAATCTTCGCTACGGCTATGAATAGAAAAGGATATTATTTATATGGATATAGACAT
    TTTTCAAGTCGTATCAAAGGTGGACATACGAATAATAAAATTAGAGTTTCTACGACGCCT
    GTTCATGCAATTAGTGATGATTTAGATATTTTGATTGCATTTGACCAAGAAACAATTGAT
    GTTAACCATCATGAAATGAGAGAAGACAGTATTATTTTAGCTGATGCCAAGGCTAAACCT
    GTGAAACCAGAAGGATGTCATGCACAGCTTATTGAATTACCTTTTACAGCAACCGCTAAA
    GAATTAGGTACAGCATTAATGAAAAACATGGTTGCAATAGGTGCTACTAGCGCATTGATG
    AATTTGAATACAAATACATTTGAAGAACTTATTACTAATATGTTTTCTAAAAAAGGTGAC
    AAGGTAGTTGAAGTCAATATCCAAGCATTAAACGAAGGTTATCAATTAATGCAATCCCGC
    TTACCTGAAATCGACGGGGACTTTGAATTAGAGTCAACAGATGCACTACCACATCTATAT
    ATGATTGGTAACGATGCCATTGGATTAGGTGCAATTGCTGCAGGTTCACAATTTATGGCG
    GCATATCCTATTACACCTGCGTCTGAAGTTATGGAATATATGATTGCCAATATATCTAAA
    GTAAACGGAGCGGTTATTCAAACAGAAGATGAAATTGCTGCTGTAACTATGGCTATTGGT
    GCAAATTATGGTGGAGTCAGAGCGTTTACGGCTAGTGCTGGTCCAGGTTTATCTTTAATG
    ATGGAAGCAATTGGATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACTCAA
    CGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATG
    ATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGAT
    GCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATA
    GTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAAT
    CGCATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGAT
    AAAGGTTATTTCAAGCGTTATGCGTTAACATCCAATGGTGTTTCTCCTAGACCTATCCCC
    GGTGTTAAAGGAGGTATTCATCATATAACTGGTGTGGAACACAATGAAGAAGGTAAACCT
    AGTGAATCTGCGTCAAATAGACAACAACAAATGGAAAAACGAATGCGTAAAATTGAGCAG
    TTACTAATTGAATCGCCAGTAGAAGCTAACTTACAACATGAGGATGCAGATATTCTTTAT
    ATCGGTTTTATTTCTACAAAAGGTGCAATTCAAGAAGGTAGTAACCGTTTGAATCAACAA
    GGCATAAAAGTTAACACTATACAAATTAGACAATTGCATCCATTCCCAACAAGCGTTATT
    CAAGATGCAGTTAATAAAGCGAAGAAAGTCGTTGTAGTGGAGCACAATTATCAAGGACAA
    TTGGCTAGTATTATAAAAATGAATGTCAATATTCATGATAAGATTGAAAATTATACAAAG
    TATGATGGGACACCTTTCCTACCACATGAAATCGAAGAAAAAGGCAAAATAATTGCTACT
    GAAATAAAGGAGATGGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1761

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS06395 [old locus tag: SA1131 ]
  • symbol: SA_RS06395
  • description: 2-oxoglutarate ferredoxin oxidoreductase subunit alpha
  • length: 586
  • theoretical pI: 5.06399
  • theoretical MW: 64506.1
  • GRAVY: -0.280375

⊟Function[edit | edit source]

  • TIGRFAM:
    2-oxoacid:acceptor oxidoreductase, alpha subunit (TIGR03710; HMM-score: 712.8)
    and 9 more
    indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 89.1)
    2-oxoacid:acceptor oxidoreductase, gamma subunit, pyruvate/2-ketoisovalerate family (TIGR02175; HMM-score: 37.1)
    indolepyruvate ferredoxin oxidoreductase, beta subunit (TIGR03334; EC 1.2.7.8; HMM-score: 25.2)
    pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 25)
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-dehydroquinate dehydratase, type I (TIGR01093; EC 4.2.1.10; HMM-score: 24)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.8)
    Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 12.1)
  • TheSEED: see SA1131
  • PFAM:
    THDP-binding (CL0254) POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 214.2)
    and 5 more
    no clan defined POR; Pyruvate ferredoxin/flavodoxin oxidoreductase (PF01558; HMM-score: 136.9)
    TKC_like (CL0591) PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 40.7)
    THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 30.1)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 24.4)
    TIM_barrel (CL0036) DHquinase_I; Type I 3-dehydroquinase (PF01487; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028246
    • TAT(Tat/SPI): 0.000914
    • LIPO(Sec/SPII): 0.001441
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSRIKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVKPEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGDKVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQFMAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPGLSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVAPTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQSDIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQMEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQIRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPFLPHEIEEKGKIIATEIKEMV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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