From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06785 [old locus tag: SA1193 ]
  • pan locus tag?: SAUPAN003749000
  • symbol: SA_RS06785
  • pan gene symbol?: mprF
  • synonym:
  • product: phosphatidylglycerol lysyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06785 [old locus tag: SA1193 ]
  • symbol: SA_RS06785
  • product: phosphatidylglycerol lysyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1363612..1366134
  • length: 2523
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1363612..1366134) NCBI
  • BioCyc: SA_RS06785 BioCyc
  • MicrobesOnline: see SA1193

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATCAGGAAGTTAAAAACAAAATATTTTCAATCTTAAAAATTACGTTTGCTACAGCT
    TTATTTATTTTTGTAGCAATCACATTGTATCGGGAGTTATCTGGTATTAACTTTAAAGAT
    ACGTTGGTTGAATTTAGTAAGATTAACCGTATGTCCTTAGTGTTACTATTTATTGGTGGT
    GGGGCATCGCTTGTTATTCTATCAATGTATGATGTGATTTTATCTAGAGCTTTAAAAATG
    GATATATCCTTAGGCAAAGTTTTAAGAGTAAGTTATATCATCAATGCATTGAATGCGATT
    GTAGGTTTCGGTGGCTTTATTGGTGCAGGCGTTAGAGCAATGGTTTATAAAAACTATACG
    CATGATAAAAAGAAATTAGTTCACTTTATATCCTTAATACTTATTTCAATGTTGACAGGT
    TTAAGCTTATTATCATTGCTAATTGTATTCCATGTTTTCGATGCATCTTTAATCTTAGAT
    AAGATTACATGGGTAAGATGGGTATTATATGTAGTGTCATTTTTCTTACCATTATTCATT
    ATTTATTCAATGGTTAGACCACCCGATAAAAACAATCGTTTTGTAGGATTGTACTGCACT
    TTAGTGTCGTGTGTTGAATGGTTAGCAGCTGCAGTTGTATTATATTTCTGTGGTGTAATT
    GTTGACGCTCATGTATCATTCATGTCCTTTATTGCAATATTTATCATTGCTGCATTATCA
    GGTTTAGTCAGCTTTATTCCTGGTGGTTTCGGCGCTTTCGATTTAGTTGTATTACTAGGA
    TTTAAAACTTTAGGTGTCCCTGAGGAAAAAGTATTATTAATGCTACTTCTATATCGTTTT
    GCGTACTATTTTGTACCGGTAATTATTGCATTAATTTTATCATCATTTGAATTTGGTACA
    TCAGCTAAGAAGTACATTGAGGGATCTAAATACTTTATTCCTGCTAAAGATGTTACGTCA
    TTTTTAATGTCTTATCAAAAGGATATTATTGCTAAAATTCCATCATTATCATTAGCAATT
    TTAGTATTCTTTACAAGTATGATCTTTTTTGTAAATAACTTAACGATTGTTTACGATGCT
    TTATATGATGGAAATCACTTAACGTATTATATTCTATTGGCAATTCATACTAGTGCTTGT
    TTATTACTTTTACTGAATGTAGTTGGTATTTATAAGCAAAGTAGACGTGCCATTATCTTT
    GCTATGATTTCAATTTTATTAATCACAGTGGCGACATTCTTCACTTACGCTTCATATATT
    TTAATAACATGGTTAGCTATTATTTTTGTTCTGCTTATTGTAGCTTTCCGTAGAGCACGT
    AGGTTGAAACGCCCAGTAAGAATGAGAAATATAGTTGCAATGCTTTTATTCAGTTTATTT
    ATTTTATATGTTAACCATATATTTATTGCTGGAACGTTATATGCATTAGATATTTATACG
    ATTGAAATGCATACATCTGTATTGCGCTATTACTTCTGGCTTACGATTTTAATCATCGCT
    ATCATCATAGGTATGATTGCATGGTTGTTTGATTATCAATTTAGCAAAGTACGTATTTCT
    TCTAAAATTGAAGATTGCGAGGAGATTATTAATCAGTACGGCGGTAATTATTTGAGTCAC
    TTGATATATAGTGGTGACAAGCAGTTTTTCACTAATGAAAATAAAACAGCATTTTTAATG
    TATCGTTATAAAGCAAGTTCATTAGTGGTTCTTGGAGATCCGTTAGGTGATGAAAATGCC
    TTTGATGAATTGTTAGAAGCATTCTATAATTACGCTGAGTATTTAGGCTATGATGTTATA
    TTCTATCAAGTTACAGATCAACACATGCCTTTATATCATAATTTCGGTAACCAATTTTTC
    AAATTAGGTGAAGAAGCAATTATTGATTTAACGCAATTTTCAACTTCAGGTAAAAAACGC
    CGTGGATTTAGAGCGACTTTAAACAAATTCGATGAACTTAATATTTCATTCGAAATTATT
    GAACCACCGTTTTCAACTGAATTTATAAATGAGCTTCAACATGTAAGTGATTTATGGCTA
    GATAATCGTCAGGAAATGCATTTCTCTGTGGGTCAATTTAATGAAGAATACTTATCTAAA
    GCGCCAATTGGTGTAATGCGAAATGAAGAAAATGAAGTAATTGCATTCTGTAGTTTAATG
    CCAACATACTTTAATGATGCCATTTCAGTCGATTTAATTAGATGGTTGCCAGAGTTAGAT
    TTACCATTAATGGATGGATTATACTTGCACATGTTACTTTGGAGTAAAGAACAAGGTTAT
    ACAAAATTTAATATGGGTATGGCAACGTTATCGAACGTTGGTCAATTGCATTATTCATAT
    TTAAGAGAACGACTTGCAGGCCGTGTCTTTGAACATTTCAACGGTCTATATCGTTTCCAA
    GGATTACGTCGTTATAAATCTAAATATAATCCGAATTGGGAACCACGCTTTTTAGTTTAT
    CGTAAAGATAATTCGCTTTGGGAATCACTTTCTAAAGTAATGCGTGTAATACGTCACAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2523

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS06785 [old locus tag: SA1193 ]
  • symbol: SA_RS06785
  • description: phosphatidylglycerol lysyltransferase
  • length: 840
  • theoretical pI: 9.04288
  • theoretical MW: 96864.7
  • GRAVY: 0.509167

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.2.3?  ExPASy
    Lysyltransferase L-lysyl-tRNA(Lys) + phosphatidylglycerol = tRNA(Lys) + 3-O-L-lysyl-1-O-phosphatidylglycerol
  • TIGRFAM:
    Unknown function General TIGR00374 family protein (TIGR00374; HMM-score: 48.1)
  • TheSEED: see SA1193
  • PFAM:
    Acetyltrans (CL0257) DUF2156; Uncharacterised conserved protein (DUF2156) (PF09924; HMM-score: 263.9)
    and 1 more
    no clan defined LPG_synthase_TM; Lysylphosphatidylglycerol synthase TM region (PF03706; HMM-score: 48.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008846
    • TAT(Tat/SPI): 0.00019
    • LIPO(Sec/SPII): 0.004849
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVEFSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVGFGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILDKITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCGVIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLLLYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIPSLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYKQSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRNIVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAWLFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKASSLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGEEAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDNRQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELDLPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYRFQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS06785&oldid=80039"