PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS07200 [old locus tag: SA1270 ]
• pan locus tag^?: SAUPAN003891000
• symbol: SA_RS07200
• pan gene symbol^?: —
• synonym:
• product: amino acid permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS07200 [old locus tag: SA1270 ]
• symbol: SA_RS07200
• product: amino acid permease
• replicon: chromosome
• strand: -
• coordinates: 1471747..1473069
• length: 1323
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1471747..1473069) NCBI
• BioCyc: SA_RS07200 BioCyc
• MicrobesOnline: see SA1270

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGTCAAATGGTAAAGAATTACAAAAAAATATAGGTTTCTTCTCAGCGTTTGCTATTGTT
  ATGGGGACAGTTATTGGTTCAGGAGTATTCTTTAAAATATCAAACGTAACAGAAGTAACA
  GGAACAGCAGGAATGGCCTTGTTTGTATGGTTCCTAGGCGGCATCATTACCATTTGTGCG
  GGGTTAACAGCAGCAGAACTTGCTGCTGCAATCCCTGAAACAGGTGGCTTAACGAAGTAT
  ATAGAATATACATACGGTGATTTCTGGGGCTTCCTATCAGGTTGGGCGCAATCATTTATT
  TATTTTCCAGCTAACGTAGCAGCATTGTCTATCGTATTTGCGACACAGCTAATTAATTTA
  TTCCATTTATCTATAGGTTCGTTAATACCAATAGCAATCGCATCTGCGTTATCTATTGTG
  TTGATAAATTTCCTAGGTTCAAAAGCAGGCGGAATTTTACAATCAGTTACTTTAGTAATT
  AAACTGATTCCAATCATCGTTATTGTAATTTTTGGTATTTTTCAATCTGGAGATATCACT
  TTTTCATTAATTCCAACTACAGGTAATTCAGGAAATGGTTTCTTTACAGCAATTGGTAGT
  GGTTTATTAGCAACTATGTTTGCATATGATGGTTGGATTCATGTAGGAAATGTTGCGGGG
  GAACTTAAAAATCCTAAACGCGATTTACCTTTAGCGATTTCAGTTGGTATCGGTTGTATT
  ATGGCTGTGTATTTATTAATTAACGCTACATTTTTATTAACGTTGCCAATAGAACTACTT
  GCTGGTAACTTAAATGCAGCTTCAGATACATCAAAAATATTATTTGGTGAAAATGGCGGT
  AAAATTATTACAATCGGTATATTAATTTCTGTTTATGGTACGATCAATGGCTATACTATG
  ACTGGTATGCGCGTACCATATGCAATGGCTGAAAGAAAATTATTGCCATTTAGCCACTTA
  TTCGCAAAATTAACAAAATCTGGCGCACCATGGTTTGGCGCAATTATACAACTTATAATC
  GCTATCATCATGATGTCAATGGGAGCATTTGATACAATAACAAACATGTTAATCTTTGTT
  ATTTGGTTGTTCTATTGTATGTCATTTGTTGCTGTAATAATTTTAAGAAAACGTGAACCA
  AATATGGAGCGTCCATATAAAGTACCGTTATATCCGATCATACCTTTAATTGCTATTTTG
  GCAGGATCATTTGTATTGATTAATACACTGTTTACACAATTTATATTAGCAATCATTGGA
  ATTCTAATAACAGCACTTGGTATACCAGTTTATTACTATAAGAAGAAACAAAAAGCAGCA
  TAA
  60
  120
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  1140
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  1260
  1320
  1323
  

⊟Protein

⊟General

• locus tag: SA_RS07200 [old locus tag: SA1270 ]
• symbol: SA_RS07200
• description: amino acid permease
• length: 440
• theoretical pI: 9.55367
• theoretical MW: 47261.2
• GRAVY: 0.935682

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 211.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 170.7)
  and 10 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 133.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 114.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 85.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 72)
  putrescine-ornithine antiporter (TIGR04299; HMM-score: 62.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (TIGR00907; HMM-score: 58.8)
  histidine-histamine antiporter (TIGR04298; HMM-score: 54.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 49.5)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 38.8)
  early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 8.2)
• TheSEED: see SA1270
• PFAM:
  APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 202.8)
  and 2 more
  AA_permease; Amino acid permease (PF00324; HMM-score: 154.4)
  AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 44.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012697
  • TAT(Tat/SPI): 0.000621
  • LIPO(Sec/SPII): 0.01798
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 445986923 NCBI
• RefSeq: WP_000064778 NCBI
• UniProt: see SA1270

⊟Protein sequence

• MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAGMALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYFPANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVIKLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNVAGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFGENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGAIIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYPIIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SA1270

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications