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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07740 [old locus tag: SA1368 ]
  • pan locus tag?: SAUPAN004116000
  • symbol: SA_RS07740
  • pan gene symbol?: aroK
  • synonym:
  • product: shikimate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07740 [old locus tag: SA1368 ]
  • symbol: SA_RS07740
  • product: shikimate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1576950..1577474
  • length: 525
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1576950..1577474) NCBI
  • BioCyc: G1G21-1557 BioCyc
  • MicrobesOnline: see SA1368

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAATCATGATAAATCACCAATAATTTTAATTGGTTTCATGGGTACCGGTAAATCTACG
    ATTGGTAAATACGTTGCAGATGAGCAAAATTTATCATTTATTGATATAGATTCATATATC
    GAAGAGAAGTATAAGTTAACAATACCAGAAATATTTAGTAAACATGGTGAACAATATTTC
    AGGAATTTAGAGTTCACATGTTTGCAAGAATGTATTAACACTGCAGATATAATTGCTACT
    GGTGGTGGTATTATTGAAAGTGAAGAGGCATTTAATTTTTTGAAAAATCAAAAAAACATT
    ATTTGGTTAGATTGTAATATTGATATTATATATAGTCGAATCAATGATGACCCACATCGA
    CCTAATGCAAATAATAAGACAATCAAGCAGTTAAATGACTTGTATTGCTCGCGGATTTTA
    AGATATAATGAAATCGCATTCAAGAAATTTGATAGTCATTTGCTATCAATTTCAGAAATA
    TATTATGAATTGCTAAATTTAATAAAAGCGAGTGATCAGTATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    525

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS07740 [old locus tag: SA1368 ]
  • symbol: SA_RS07740
  • description: shikimate kinase
  • length: 174
  • theoretical pI: 4.87103
  • theoretical MW: 20204.8
  • GRAVY: -0.317241

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.71?  ExPASy
    Shikimate kinase ATP + shikimate = ADP + shikimate 3-phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A dephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 20)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 19.5)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 17)
    and 1 more
    AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 9.5)
  • TheSEED: see SA1368
  • PFAM:
    P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 145.1)
    and 6 more
    AAA_18; AAA domain (PF13238; HMM-score: 32.1)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 20.5)
    AAA_33; AAA domain (PF13671; HMM-score: 16.5)
    AAA_22; AAA domain (PF13401; HMM-score: 16.2)
    CoaE; Dephospho-CoA kinase (PF01121; HMM-score: 12.2)
    AAA_24; AAA domain (PF13479; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020471
    • TAT(Tat/SPI): 0.000764
    • LIPO(Sec/SPII): 0.018107
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKYKLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIWLDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEIYYELLNLIKASDQY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS06005peptide deformylase  [1] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [1] (data from MRSA252)
    SA_RS11010uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [1] (data from MRSA252)
    SA_RS13340pyruvate oxidase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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