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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08960 [old locus tag: SA1590 ]
  • pan locus tag?: SAUPAN004460000
  • symbol: SA_RS08960
  • pan gene symbol?:
  • synonym:
  • product: pyridine nucleotide-disulfide oxidoreductase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08960 [old locus tag: SA1590 ]
  • symbol: SA_RS08960
  • product: pyridine nucleotide-disulfide oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 1828799..1830301
  • length: 1503
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1828799..1830301) NCBI
  • BioCyc: SA_RS08960 BioCyc
  • MicrobesOnline: see SA1590

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTGTAGCAATAATAGGCATGGGAACTGCTGGTGTAAGTGTATTGAGAGAGTTAGTT
    AAGCACCCGAAGTTTAATCAATTAGATATAGATTTATACGATGATAAAGTAAATATGGGA
    CAGGGCGTACCATTTCAAAATGATAGTTCTGAGCTGCTAATTAATATGCCTTCGAAAAAG
    ATGAGTTTAAATTTAGATGATGAGACTGAATTTTGGAAGTGGTATAAACAACAAACTGAT
    TTTAATTTTGATGAACCTGCATATTTACCGAGATTTGTATTCGGACATTATATGAAATCT
    TATTTATCTATGTTCACGAAAAAATATCCAAATATATCAACTAATTATAATAAAGTTCAA
    GAGATTTATACAAATTCTAATATCGATGAAACGAATTTAACTTATTACATATGTACTACA
    AATTCAGAACAATCATGGCAAGCGTACGATTATGTATTTTTAACATGTGGTACATTTGCC
    TACCATGATCCATATAACTTAAAGGGTAAAAAAGGATATATCGCTACGCCTTATCCAACA
    TATAATACGTTAGACGAAGTAAATGAACTGGACGATATTGCGATTATTGGTACAGGCCTT
    GCCAGTTTAGATGTTGTCCGTTATGTTGCTGCTCATCATCCGAAATTACCAATTACTATG
    ACGAGTCGTTCTGCTCATTTACCAAGTGTAAGAGGTACAATGATAGACGTGACATTCAAG
    TACTTAACTAAAGACAAATTAAATGACATTAAAAAACATCATTTCGGCAATGCACCGCTT
    GATACTATAGTTTCTTTATTTTTAAAAGAATGTGCTGAATATGACATTGATTTTAAAAAA
    TTAGTACATAGACGTACAGGAAATCATATTGCAGATTTGAAGTATGATTTAGCGCGTCCA
    ACAGAAATGGGCATTTTCCAAAGTATGATTGAACATTTGAAAGAGAATTTAAATTGGATA
    TGGAATAGCTTAAGTATTGAAGATCAACATCAATTTAATCAAAAATACTCAAAGATGATT
    CAATTAAATTCAAATCCAATGCCTCCAAGAACTGCTGAACTAATTATTGAATTAATAGAA
    AATAAGTCTTTAATTTTGAAAAAAGATTTAGAAGATGTGAAACATGATGGCAAGTTGTAT
    TATTTTTCATACAAAAACCAAGAATCAGTAGACATGTATAATGTTGTCATTAACGCGACA
    GGTGCAAAAAGTCATTTAAATGAATTGGATCAAGATGATCAATTAATTAAAAATCTTGAA
    AATAGACAAATTGTACAAGCACATCCAATGGGAGGCATACAAATCATACCGGAAACCAAT
    CAAGTTATTAGTCCAAGGTTTGGAACATTAACAAATATGATTGCGATAGGTCAAATGACA
    AACGGTGTTAATAAACTACGAAATGGTGTGAAAATGATAGTAGAACAAGTTGCGCATACA
    GTATCTCAATTATATGATGCTTTAGAATCGAATGAGCAACAACAGCGCAGTTACAATCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1503

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS08960 [old locus tag: SA1590 ]
  • symbol: SA_RS08960
  • description: pyridine nucleotide-disulfide oxidoreductase
  • length: 500
  • theoretical pI: 6.41232
  • theoretical MW: 57687.2
  • GRAVY: -0.4794

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 15.4)
    salicylate 1-monooxygenase (TIGR03219; EC 1.14.13.1; HMM-score: 14.9)
  • TheSEED: see SA1590
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 113.1)
    and 6 more
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 33.6)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 24.8)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 21)
    Thi4; Thi4 family (PF01946; HMM-score: 15.3)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 15.1)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 12.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016499
    • TAT(Tat/SPI): 0.000305
    • LIPO(Sec/SPII): 0.005268
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVPFQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLSMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFAYHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTIVSLFLKECAEYDIDFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDMYNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSYNQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]