PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08960 [old locus tag: SA1590 ]
• pan locus tag^?: SAUPAN004460000
• symbol: SA_RS08960
• pan gene symbol^?: —
• synonym:
• product: pyridine nucleotide-disulfide oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08960 [old locus tag: SA1590 ]
• symbol: SA_RS08960
• product: pyridine nucleotide-disulfide oxidoreductase
• replicon: chromosome
• strand: -
• coordinates: 1828799..1830301
• length: 1503
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1828799..1830301) NCBI
• BioCyc: SA_RS08960 BioCyc
• MicrobesOnline: see SA1590

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  1501
  
  ATGCGTGTAGCAATAATAGGCATGGGAACTGCTGGTGTAAGTGTATTGAGAGAGTTAGTT
  AAGCACCCGAAGTTTAATCAATTAGATATAGATTTATACGATGATAAAGTAAATATGGGA
  CAGGGCGTACCATTTCAAAATGATAGTTCTGAGCTGCTAATTAATATGCCTTCGAAAAAG
  ATGAGTTTAAATTTAGATGATGAGACTGAATTTTGGAAGTGGTATAAACAACAAACTGAT
  TTTAATTTTGATGAACCTGCATATTTACCGAGATTTGTATTCGGACATTATATGAAATCT
  TATTTATCTATGTTCACGAAAAAATATCCAAATATATCAACTAATTATAATAAAGTTCAA
  GAGATTTATACAAATTCTAATATCGATGAAACGAATTTAACTTATTACATATGTACTACA
  AATTCAGAACAATCATGGCAAGCGTACGATTATGTATTTTTAACATGTGGTACATTTGCC
  TACCATGATCCATATAACTTAAAGGGTAAAAAAGGATATATCGCTACGCCTTATCCAACA
  TATAATACGTTAGACGAAGTAAATGAACTGGACGATATTGCGATTATTGGTACAGGCCTT
  GCCAGTTTAGATGTTGTCCGTTATGTTGCTGCTCATCATCCGAAATTACCAATTACTATG
  ACGAGTCGTTCTGCTCATTTACCAAGTGTAAGAGGTACAATGATAGACGTGACATTCAAG
  TACTTAACTAAAGACAAATTAAATGACATTAAAAAACATCATTTCGGCAATGCACCGCTT
  GATACTATAGTTTCTTTATTTTTAAAAGAATGTGCTGAATATGACATTGATTTTAAAAAA
  TTAGTACATAGACGTACAGGAAATCATATTGCAGATTTGAAGTATGATTTAGCGCGTCCA
  ACAGAAATGGGCATTTTCCAAAGTATGATTGAACATTTGAAAGAGAATTTAAATTGGATA
  TGGAATAGCTTAAGTATTGAAGATCAACATCAATTTAATCAAAAATACTCAAAGATGATT
  CAATTAAATTCAAATCCAATGCCTCCAAGAACTGCTGAACTAATTATTGAATTAATAGAA
  AATAAGTCTTTAATTTTGAAAAAAGATTTAGAAGATGTGAAACATGATGGCAAGTTGTAT
  TATTTTTCATACAAAAACCAAGAATCAGTAGACATGTATAATGTTGTCATTAACGCGACA
  GGTGCAAAAAGTCATTTAAATGAATTGGATCAAGATGATCAATTAATTAAAAATCTTGAA
  AATAGACAAATTGTACAAGCACATCCAATGGGAGGCATACAAATCATACCGGAAACCAAT
  CAAGTTATTAGTCCAAGGTTTGGAACATTAACAAATATGATTGCGATAGGTCAAATGACA
  AACGGTGTTAATAAACTACGAAATGGTGTGAAAATGATAGTAGAACAAGTTGCGCATACA
  GTATCTCAATTATATGATGCTTTAGAATCGAATGAGCAACAACAGCGCAGTTACAATCAA
  TAA
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  1503
  

⊟Protein

⊟General

• locus tag: SA_RS08960 [old locus tag: SA1590 ]
• symbol: SA_RS08960
• description: pyridine nucleotide-disulfide oxidoreductase
• length: 500
• theoretical pI: 6.41232
• theoretical MW: 57687.2
• GRAVY: -0.4794

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 15.4)
  salicylate 1-monooxygenase (TIGR03219; EC 1.14.13.1; HMM-score: 14.9)
• TheSEED: see SA1590
• PFAM:
  NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 113.1)
  and 6 more
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 33.6)
  Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 24.8)
  K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 21)
  Thi4; Thi4 family (PF01946; HMM-score: 15.3)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 15.1)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016499
  • TAT(Tat/SPI): 0.000305
  • LIPO(Sec/SPII): 0.005268
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447184412 NCBI
• RefSeq: WP_001261668 NCBI
• UniProt: see SA1590

⊟Protein sequence

• MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVPFQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLSMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFAYHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTIVSLFLKECAEYDIDFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDMYNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSYNQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications