PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS09140 [old locus tag: SA1624 ]
• pan locus tag^?: SAUPAN004533000
• symbol: SA_RS09140
• pan gene symbol^?: tnp
• synonym:
• product: IS21 family transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS09140 [old locus tag: SA1624 ]
• symbol: SA_RS09140
• product: IS21 family transposase
• replicon: chromosome
• strand: -
• coordinates: 1856978..1857478
• length: 501
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (1856978..1857478) NCBI
• BioCyc: SA_RS09140 BioCyc
• MicrobesOnline: see SA1624

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTATCTTTAGACATAAATACTGATTTTGAAGTTACTACTCTTACAGATTTACCA
  AAATTAAAAATTGTCATGGAGAACTTAAACATGAAAATAAACAAAAGTGAAATAGCGAGA
  CACATGGGTGTTTATAGAAGAACAGTTGATAAATATTTAAATGGCTTTGAGCCAACCAAA
  AAGAGAAATCGTCAGTCTATTATTGACAAGTATTATCCTATCATCGAAAAATTACTTTCA
  GACTCTAGTGAACAAAAGTTCTACTATAAGCTTATTCTTTGGCAATATTTGAAAGATAAA
  CATGGATTAACCTGTGCATATTCTACATTTAGAGCTTATATCCTTAAACATGATGAATTT
  AATCGTTATTTTATGAAAGGTTATCAACGGTTGTCGCCTAAAGGCAAAACGAGATTCGAG
  ACAAAAGCCAGTCATCAAGCACAGTTTGACTGGAAAGAAGGCATTAACTTTAAAACGAAG
  GATAATCAAATGGTCCTTTAG
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  501
  

⊟Protein

⊟General

• locus tag: SA_RS09140 [old locus tag: SA1624 ]
• symbol: SA_RS09140
• description: IS21 family transposase
• length: 166
• theoretical pI: 10.1619
• theoretical MW: 19944
• GRAVY: -0.73494

⊟Function

• TIGRFAM:
• TheSEED: see SA1624
• PFAM:
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 23)
  and 5 more
  no clan defined DUF5338; Family of unknown function (DUF5338) (PF17273; HMM-score: 17.3)
  HTH (CL0123) HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 16.7)
  no clan defined Phage_NinH; Phage NinH protein (PF06322; HMM-score: 16.1)
  HTH (CL0123) HTH_29; Winged helix-turn helix (PF13551; HMM-score: 14.4)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004248
  • TAT(Tat/SPI): 0.000149
  • LIPO(Sec/SPII): 0.00027
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446700128 NCBI
• RefSeq: WP_000777474 NCBI
• UniProt: see SA1624

⊟Protein sequence

• MKLSLDINTDFEVTTLTDLPKLKIVMENLNMKINKSEIARHMGVYRRTVDKYLNGFEPTKKRNRQSIIDKYYPIIEKLLSDSSEQKFYYKLILWQYLKDKHGLTCAYSTFRAYILKHDEFNRYFMKGYQRLSPKGKTRFETKASHQAQFDWKEGINFKTKDNQMVL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications