PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12430 [old locus tag: SA2164 ]
• pan locus tag^?: SAUPAN005894000
• symbol: SA_RS12430
• pan gene symbol^?: —
• synonym:
• product: phage infection protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12430 [old locus tag: SA2164 ]
• symbol: SA_RS12430
• product: phage infection protein
• replicon: chromosome
• strand: -
• coordinates: 2430705..2431982
• length: 1278
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2430705..2431982) NCBI
• BioCyc: SA_RS12430 BioCyc
• MicrobesOnline: see SA2164

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGAATATATTTAAAAATAAATTATTATGGATTGCGCCAATAGCAACTATGATTATCTTG
  GTAATCTTTTCTTTAGCTTTTTATCCTGCATATAATCCTAAACCAAAAGATTTACCAATT
  GGTATATTAAACGAGGATAAAGGTACAACGATTCAAGATAAAAATGTTAACATTGGTAAA
  AAATTAGAGGATAAATTATTAGATAGTGATTCTAATAAAATTAAATGGGTTAAGGTTGAT
  AGTGAAAAAGACCTTGAAAAAGATTTGAAAGATCAAAAAATCTTTGGAGTAGCTATTATT
  GATAAAGACTTTTCAAAAGATGCTATGAGTAAAACACAAAAAGTAGTTATGGATAGTAAA
  AAAGAAGAAATGCAACAAAAAGTTGCTTCAGGTGAAATTCCGCCACAAGTGGTTCAACAA
  ATGAAACAAAAAATGGGGAATCAACAAGTAGAGGTTAAGCAGGCTAAATTTAAAACGATT
  GTAAGTGAAGGATCAAGCTTACAAGGTTCACAAATTGCATCAGCTGTGTTAACTGGTATG
  GGTGATAATATTAATGCTCAAATTACGAAGCAAAGTTTGGAAACATTAACGAGTCAAAAT
  GTTAAAGTCAATGCCGCGGACATCAATGGTTTGACGAATCCAGTAAAAGTGGATAATGAA
  AAACTTAATAAAGTTAAAGATCACCAAGCAGGTGGTAATGCACCATTCCTAATGTTTATG
  CCAATTTGGATAGGTTCAATCGTAACGTCTATCTTATTGTTCTTTGCATTTAGAACTAGT
  AACAATATCGTCGTGCAACATCGTATCATTGCTTCAATTGGACAGATGATATTTGCAGTT
  GTTGCAGCATTTGCAGGTAGCTTTGTTTATATTTATTTCATGCAAGGCGTTCAAGGATTT
  GATTTTGACCATCCAAATCGTATCGCAATTTTTGTAGCATTTGCGATTCTTGGTTTCGTG
  GGCCTTATTTTAGGTGTTATGGTATGGCTAGGTATGAAGTCAGTTCCAATTTTCTTCATT
  TTAATGTTCTTTAGTATGCAACTTGTAACGTTACCTAAACAAATGTTGCCTGAAAGTTAT
  CAAAAATATGTATATGATTGGAATCCATTCACACACTATGCAACAAGTGTAAGAGAACTA
  TTATACTTGAATCATCATATTGAATTAAATAGTACAATGTGGATGTTTATAGGGTTTATG
  ATTTTTGGCGCTGTATCAAGTTTAGTTTCAGCTATTGTTAGAAAACATAGCACAAAACGT
  ACTGAAGTGCCGTCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
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  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1278
  

⊟Protein

⊟General

• locus tag: SA_RS12430 [old locus tag: SA2164 ]
• symbol: SA_RS12430
• description: phage infection protein
• length: 425
• theoretical pI: 9.91365
• theoretical MW: 47727.7
• GRAVY: 0.107294

⊟Function

• TIGRFAM:
  YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 71.6)
  and 4 more
  YhgE/Pip C-terminal domain (TIGR03062; HMM-score: 57.2)
  type VII secretion protein EsaA (TIGR03929; HMM-score: 25.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 17.7)
  ferredoxin-type protein, NapH/MauN family (TIGR02163; HMM-score: 12.8)
• TheSEED: see SA2164
• PFAM:
  ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 100.1)
  and 4 more
  no clan defined DUF1598; Protein of unknown function (DUF1598) (PF07643; HMM-score: 13)
  DIMCO_N; Dinitrogenase iron-molybdenum cofactor, N-terminal (PF16844; HMM-score: 12.4)
  NADP_Rossmann (CL0063) Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 12.1)
  no clan defined Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 6.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.9979
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0019
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.403898
  • TAT(Tat/SPI): 0.001497
  • LIPO(Sec/SPII): 0.027886
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 446942760 NCBI
• RefSeq: WP_001020016 NCBI
• UniProt: see SA2164

⊟Protein sequence

• MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILNEDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDKDFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTIVSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVDNEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQMIFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKSVPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNSTMWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications