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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13250 [old locus tag: SA2311 ]
  • pan locus tag?: SAUPAN006168000
  • symbol: SA_RS13250
  • pan gene symbol?: frp
  • synonym:
  • product: NAD(P)H-dependent oxidoreductase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13250 [old locus tag: SA2311 ]
  • symbol: SA_RS13250
  • product: NAD(P)H-dependent oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2596052..2596723
  • length: 672
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2596052..2596723) NCBI
  • BioCyc: G1G21-2691 BioCyc
  • MicrobesOnline: see SA2311

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGCAATATGAATCAAACAATTATGGATGCATTTCATTTCAGACATGCGACTAAGCAA
    TTCGATCCACAAAAGAAAGTTTCGAAAGAAGATTTTGAAACAATATTAGAGTCAGGTAGA
    TTGTCTCCAAGTTCTCTTGGGTTAGAACCTTGGAAGTTTGTCGTGATTCAAGATCAAGCG
    TTACGTGATGAATTAAAAGCGCACAGTTGGGGCGCAGCAAAACAATTAGATACAGCGAGC
    CATTTTGTGCTAATTTTTGCGCGTAAAAATGTAACGTCAAGATCACCGTATGTACAACAT
    ATGTTAAGAGATATTAAAAAATATGAGGCACAAACGATCCCAGCTGTTGAACAAAAATTC
    GATGCATTCCAAGCAGATTTCCATATTTCTGATAATGATCAAGCCTTGTATGACTGGTCA
    AGTAAACAAACGTATATCGCATTAGGCAATATGATGACGACAGCCGCATTGTTAGGTATT
    GATTCATGTCCGATGGAAGGTTTTAGTCTGGATACAGTGACAGACATTTTAGCAAATAAA
    GGTATCTTAGATACTGAGCAATTTGGTTTATCAGTGATGGTTGCATTTGGCTACAGACAA
    CAAGATCCACCGAAAAATAAAACACGCCAAGCATATGAAGATGTTATTGAATGGGTTGGA
    CCAAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    672

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS13250 [old locus tag: SA2311 ]
  • symbol: SA_RS13250
  • description: NAD(P)H-dependent oxidoreductase
  • length: 223
  • theoretical pI: 5.61876
  • theoretical MW: 25374.6
  • GRAVY: -0.43722

Function[edit | edit source]

  • reaction:
    EC 1.-.-.-?  ExPASy
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5,6-dimethylbenzimidazole synthase (TIGR02476; EC 1.14.99.40; HMM-score: 20)
  • TheSEED: see SA2311
  • PFAM:
    FMN-dep-NRtase (CL0529) Nitroreductase; Nitroreductase family (PF00881; HMM-score: 107.4)
    and 1 more
    TM1586_NiRdase; Putative TM nitroreductase (PF14512; HMM-score: 21.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002464
    • TAT(Tat/SPI): 0.00023
    • LIPO(Sec/SPII): 0.000371
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPSSLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHMLRDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGIDSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEWVGPKE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]