PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS13990 [old locus tag: SA2441 ]
• pan locus tag^?: SAUPAN006381000
• symbol: SA_RS13990
• pan gene symbol^?: gtfA
• synonym:
• product: accessory Sec system glycosyltransferase GtfA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS13990 [old locus tag: SA2441 ]
• symbol: SA_RS13990
• product: accessory Sec system glycosyltransferase GtfA
• replicon: chromosome
• strand: -
• coordinates: 2745947..2747455
• length: 1509
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2745947..2747455) NCBI
• BioCyc: SA_RS13990 BioCyc
• MicrobesOnline: see SA2441

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
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  1501
  
  ATGACAATATATAATATAAACTTTGGTATAGGCTGGGCGAGTAGCGGTGTTGAATATGCA
  CAAGCTTACCGTGCTAAATTATTGAGACGTACAAATCAAGACACTAAATTTGTGTTTTTA
  GATTTTATACAGTCAGAGAACATACAGACATTAACGCAAAACATTGGGTTTAAAGATCAA
  GAAGTTATTTGGTTGTATCAGTATTTTAGTGATATTTCTATAGCACCAACAACGTACACG
  TTAGATGACATTGAACGTGAGCTTGGTCATGAAATTAGTTTTAGAGAAAGAAATGGTAAG
  ATAGTCCGTCTATATTTTAATGGGAAATCATCATTTGTAACATGTTATCTACAAAATGAA
  CAAAAAGATGTTGTGGATCGTGCAGAATTTGTAATTAATAGTATGTTAGTACGTAAAGAT
  TTTTATAGTTATACGCGTATATTTTCTGAGTATTATGCACCAGCTGATAATAAAGCGAAA
  TTATATATGCGACAATTCTATAATGAAGATGGTTCCATCGCTTATAACGAATATATTGAT
  GGAGACAGCTCTATATATGTGTTTGAAGATGCGGTGTTGTATAACAAAACGGAATTTATT
  GCATACTTTTTACAACGATTGAATTTAACCCGAGACGATATTGTCATATTAGACCGCGCT
  TCTGATATTGGTCAGGCTGTTTTGCAGCATAAAGGTGATAGTAAAGTTGGTGTTGTGATT
  CATGCTGATCATTATAGTAATAATATGATGAGCGAGCAGCATATACTATGGAACAATTAT
  TATGAATATCAATTTTCAAAAGCTAAATACATTGATTTCTTTATTACAGCAACAGATATT
  CAAAATCATATGGTTTGTCGACAATTTGAGCAATATCAAGGTTATCGTCCACGTGTCTAT
  ACTATTCCGGTGGGAAGCATTGATGCGTTATCATATCCTAAATTATCAAGAAAGCCATAT
  GCGATGATTAGTGCATCTCGTTTAGCAAATGAGAAACATATTGATTGGCTTGTCAAAGCG
  GTTATCGTGGCTAAACGACAAGTGCCAGAATTAACATTTGATATTTATGGAGAAGGTAGC
  GAGAAGACGCGACTGCGTAAAATTATTGATACACACCGCGCACAAGATTATATTCGTTTA
  CTAGGGCATGTTAAACTTGATGAAATTTATAATGATTATGAGTTATTTCTTTCTGCATCA
  ACTAGCGAAGGTTTTGGGTTAACACTAATGGAAGCGGTAGGCTCAGGACTAGGTATGATT
  GGATTAAATGTGAATTATGGTAACCCAACCTTTATTCGAGATGGGGAAAATGGCTACTTG
  GTACCTTTTGATACCGACGAAGATAGTGTGGATGATGTTATCGCAAAACTAGCTCATGCC
  ATTGTAATGTATTTTAACAATGGTCCCCAGGCACCACATGACATCTCTTATGAAGTGGCA
  CAGCAATTTATGACTCAAGATATTATTTTAAAATGGGAAACATTAGTTCAAGAGGTGCTA
  CATGATTAA
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  1509
  

⊟Protein

⊟General

• locus tag: SA_RS13990 [old locus tag: SA2441 ]
• symbol: SA_RS13990
• description: accessory Sec system glycosyltransferase GtfA
• length: 502
• theoretical pI: 4.91958
• theoretical MW: 58270.3
• GRAVY: -0.312351

⊟Function

• reaction:
  EC 2.4.1.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 872.4)
  and 11 more
  sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 56.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 50.7)
  colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 37.7)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 21.9)
  glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 21)
  sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 19.1)
  D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 18.4)
  PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 16.8)
  Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 15.3)
  sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 11.6)
  putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 10.8)
• TheSEED: see SA2441
• PFAM:
  GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 86.4)
  and 4 more
  Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 65.6)
  no clan defined DUF5503; Family of unknown function (DUF5503) (PF17607; HMM-score: 14.5)
  DUF4304; Domain of unknown function (DUF4304) (PF14137; HMM-score: 13.8)
  GT-B (CL0113) Glyco_trans_1_3; Glycosyl transferase family 1 (PF13528; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014868
  • TAT(Tat/SPI): 0.000543
  • LIPO(Sec/SPII): 0.002457
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446080568 NCBI
• RefSeq: WP_000158423 NCBI
• UniProt: see SA2441

⊟Protein sequence

• MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQSENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIVRLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAKLYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILDRASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFITATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHIDWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYNDYELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSVDDVIAKLAHAIVMYFNNGPQAPHDISYEVAQQFMTQDIILKWETLVQEVLHD

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications