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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1032 [new locus tag: SACOL_RS05265 ]
  • pan locus tag?: SAUPAN003211000
  • symbol: SACOL1032
  • pan gene symbol?: comK1
  • synonym:
  • product: ComK family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1032 [new locus tag: SACOL_RS05265 ]
  • symbol: SACOL1032
  • product: ComK family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1042540..1043070
  • length: 531
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGTTATTCGACCAGCATTTGATGATGACGATCAAAGAAACGGTAGTGAAATAATTCGG
    TTTGACAAAACGCGTATTCAAAATCCTTTTAAAGTCCAGAAAATCATTGAACGCTCTTGC
    AAATTTTATGGTAATACTTATCTTGGCAAGAAAGCAGAGACAAACCGCATTACTGGCATT
    TCTAGTAAACCACCTATTTTACTAACACCATTATTTCCAACTTATTTTTTCCCAACACAT
    TCTGACAGACAAAATGAAAATATTTGGTTAAATATGCATTATATCGAAAGTATTAAAGAA
    TTAAAAAATCGTAAATGTAAAGTGACATTTATTAATAATGAATCAATCATTCTTCATGTT
    TCATACCACAGTTTATGGCATCAATATAACAATTCCATTTTTTACTATTACATGGTAGAT
    AAACAATCTCGCATGATATCAAAAAATCCCGACCAACCAATAGATTATAATAAAGCCACA
    TTGAATGTGTTTGAAGCATTGACACGCTATTCTTTATTTGAAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    531

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1032 [new locus tag: SACOL_RS05265 ]
  • symbol: SACOL1032
  • description: ComK family protein
  • length: 176
  • theoretical pI: 9.55605
  • theoretical MW: 21032.9
  • GRAVY: -0.647159

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Competence transcription factor
  • PFAM:
    PLP_aminotran (CL0061) ComK; ComK protein (PF06338; HMM-score: 159.3)
    and 1 more
    no clan defined Gemin7; Gem-associated protein 7 (Gemin7) (PF11095; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022157
    • TAT(Tat/SPI): 0.006136
    • LIPO(Sec/SPII): 0.003428
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKVQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIWLNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMISKNPDQPIDYNKATLNVFEALTRYSLFEDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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