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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1629 [new locus tag: SACOL_RS08305 ]
  • pan locus tag?: SAUPAN004155000
  • symbol: SACOL1629
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1629 [new locus tag: SACOL_RS08305 ]
  • symbol: SACOL1629
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1659132..1659830
  • length: 699
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGTGTCGGTATTCTAATTTTTGTCATATCAGTGATCATTTCTATCATTACTACTATG
    CGCGAAAATAGTCATAAAGATAGACAAAATCAAAAGCCACCTCAAAAAACATCTACCGAT
    AATGAACCAAAAAAAGGTGGCTTTTTTGAAGAAATTGAGCGAACGTTTAAAGAAATAAGT
    GAAGAATTAAATGAAGAAGAAAAGAAATCATCGAAACGAAAATATGATGATACGTTACCA
    CCTTTATTCGATGAACTTCCAAAGGAAGAGCCTAAATCGAAACCTGTTGTAGAACCTATG
    GCACCTAAAAAACAACAAGAAACAAAACCGATGACAGAGAAACCAATCACAGTGCCTAAA
    GCAGAACCGGTGGAGCAGAAACATAGACCTTCTAGACAAGATAATTCTGACGAAATTAGA
    CGTCAATTAGAAAAATCACTTAGAGATGATATTAAAACGATTCGTACTGACATTGATAGA
    GAAAAAGAAAAGCAAATTGCTAAAATGGAAAAACGTGCTAGAGATATTATTGAGGATAAA
    TACTTATCTGAACGTACAAAACGTTTGAAATTAAAGCAGCTGCTTAATTCTCAAAATGTC
    GAAAAAGATTTGACTAAATCAGCGTTCCAATTTGATAAAGATGAAGTAATCAATGGTATG
    ATATGGTCAGAAATTTTAGCTAAACCAAAACAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    699

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1629 [new locus tag: SACOL_RS08305 ]
  • symbol: SACOL1629
  • description: hypothetical protein
  • length: 232
  • theoretical pI: 9.69683
  • theoretical MW: 27256
  • GRAVY: -1.13578

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis putative immunoglobulin-blocking virulence protein (TIGR04526; HMM-score: 17.2)
    and 2 more
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 11)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 4.6)
  • TheSEED  :
    • Uncharacterized membrane anchored protein YqfB
  • PFAM:
    no clan defined EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 15.4)
    PulG; Type II secretory pathway pseudopilin (PF11773; HMM-score: 12.8)
    and 4 more
    DUF2897; Protein of unknown function (DUF2897) (PF11446; HMM-score: 11.6)
    IncA; IncA protein (PF04156; HMM-score: 10.2)
    SICA_like (CL0474) SICA_alpha; SICA extracellular alpha domain (PF12887; HMM-score: 7.7)
    no clan defined Ndc1_Nup; Nucleoporin protein Ndc1-Nup (PF09531; HMM-score: 5.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.5
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.351133
    • TAT(Tat/SPI): 0.001289
    • LIPO(Sec/SPII): 0.216154
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPKKGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAPKKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDREKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVINGMIWSEILAKPKQL

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]