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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL
locus tag: SACOL_RS00875 [old locus tag: SACOL0172 ]
pan locus tag^?: SAUPAN001029000
symbol: SACOL_RS00875
pan gene symbol^?: —
synonym:
product: isochorismatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS00875 [old locus tag: SACOL0172 ]
symbol: SACOL_RS00875
product: isochorismatase
replicon: chromosome
strand: -
coordinates: 198038..198595
length: 558
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (198038..198595) NCBI
BioCyc: G1G4B-183 BioCyc
MicrobesOnline: see SACOL0172

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
ATGATTAATTTTAATAAAACCGCTTTAGTGTTAATCGACCTGCAAGAAGGTATTCTTAAA
ATGGATTATGCCCCATATACAGCTGAAAATGTCGTTCAAAACGCTAATAAATTAATAGAT
GTTTTTAGAAAAAACAATGGCTTTATCGCTTTTGTTCGCGTGAATTTCTATGATGGTAAA
GATGTATTGCAACCAAATGCAATGATCTCATTACCACCAAAAGAAGGCGACGACTACAGT
CGTTTCCATCATTTATTAGACAAGAGAGATGACGATTTTGTCATAGACAAACGACATTTT
AGTGCATTTGTAGGAACAGATTTGGACTTACAATTGCGACGTCGAGGAATTGATACGATT
GTTCTTGGTGGTGTCGCAACGCATATTGGCGTAGATACGACAGCGCGAGATGCCTATCAA
TTAAACTACAATCAGTTTTTTGTTACAGATATGATGAGTGCACAAAACGAAACGCTACAT
CAATTTCCAATAGATAATGTATTCCCATTGATGGGACAAACAATAACTACAAACGACTTT
CTAAATATATTGAACTAA
60
120
180
240
300
360
420
480
540
558

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL_RS00875 [old locus tag: SACOL0172 ]
symbol: SACOL_RS00875
description: isochorismatase
length: 185
theoretical pI: 4.95766
theoretical MW: 21150.9
GRAVY: -0.195135

⊟Function[edit | edit source]

reaction:
EC 3.3.2.1? ExPASy
Isochorismatase Isochorismate + H₂O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
TIGRFAM:
pyrimidine utilization protein B (TIGR03614; HMM-score: 55)
TheSEED: see SACOL0172
PFAM:
no clan defined Isochorismatase; Isochorismatase family (PF00857; HMM-score: 132)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005264
- TAT(Tat/SPI): 0.000147
- LIPO(Sec/SPII): 0.001013
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446529544 NCBI
RefSeq: WP_000606890 NCBI
UniProt: see SACOL0172

⊟Protein sequence[edit | edit source]

MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRKNNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSAFVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLHQFPIDNVFPLMGQTITTNDFLNILN

⊟Experimental data[edit | edit source]

experimentally validated: data available for NCTC8325
protein localization:
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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