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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00747
  • pan locus tag?: SAUPAN002638000
  • symbol: SAOUHSC_00747
  • pan gene symbol?: sstB
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00747
  • symbol: SAOUHSC_00747
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 732002..732958
  • length: 957
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCGCAACAATAATAAAAAAATAATGCTTTTAATTGCAGTAACGTTATTAATTAGTATG
    CTGTACTTATTTGTAGGTATTGATTTTGAAATATTTGAATATCAATTTTCAAGTCGTTTA
    AGAAAGTTCATATTAATTATTTTAGTAGGTGCTGCCATTGCAACTTCAGTGGTGATTTTT
    CAAGCGATTACAAATAACCGTCTATTGACACCATCAATAATGGGGTTAGATGCAGTTTAT
    TTATTTATCAAAGTATTGCCAGTCTTTTTATTTGGAATTCAATCGGTATGGGTTACTAAT
    GTATATTTGAACTTTATATTAACACTTATAACGATGGTGTTATTCGCACTAATCCTATTC
    CAAGGTATCTTTAAAATCGGACATTTTTCAATTTATTTTATCTTACTTATTGGTGTCCTT
    TTAGGAACATTTTTTAGAAGCATAACAGGTTTTATTCAACTGATTATGGATCCTGAGTCA
    TTTTTAGCAATACAAAGTAGTATGTTTGCTAATTTTAATGCTTCTAATTCGAATTTAGTT
    ACTTTCTCAGCAGTGCTATTAGTAATCTTATTAGTCATTACAATTTTACTATTGCCTTAT
    TTAGATGTATTGCTTTTAGGTCGTGCTGAAGCAATTAATCTTGGGATATCGTATGAAAAA
    TTAACGCGAATTCTACTTGTAATAGTCTCAGTTTTAGTTTCTGTGTCAACTGCATTAGTA
    GGACCAATTACATTTTTAGGTTTATTAACTGTAAATCTAGCGCATGAACTAATGAAGACG
    TATGAACATAAGTATATTTTAATTGCGACAATTTGCTTGAGTTGGATTAGTTTATTTAGT
    GCGCAATGGGTAGTTGAAAGTGTGTTTGAAGCTACGACAGAAATGAGTATACTTATTGAT
    TTGATTGGTGGAAGTTATTTCATTTATCTATTAGTTAGAAGGAGAAATGCGCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00747
  • symbol: SAOUHSC_00747
  • description: hypothetical protein
  • length: 318
  • theoretical pI: 9.18406
  • theoretical MW: 35749.9
  • GRAVY: 1.17987

Function[edit | edit source]

  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 45.1)
  • TheSEED  :
    • Iron compound ABC uptake transporter permease protein
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Iron compound ABC uptake transporter permease protein
    and 1 more
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Iron acquisition in Streptococcus  Iron compound ABC uptake transporter permease protein
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 173.2)
    and 1 more
    no clan defined TMEM141; TMEM141 protein family (PF15110; HMM-score: 13.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008382
    • TAT(Tat/SPI): 0.001361
    • LIPO(Sec/SPII): 0.005901
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFILIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVYLNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPESFLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISYEKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWISLFSAQWVVESVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]