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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01916
  • pan locus tag?: SAUPAN004511000
  • symbol: SAOUHSC_01916
  • pan gene symbol?: menE
  • synonym:
  • product: 2-succinylbenzoate-CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01916
  • symbol: SAOUHSC_01916
  • product: 2-succinylbenzoate-CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1824272..1825750
  • length: 1479
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGACTTTTGGTTATATAAACAAGCACAACAAAATGGACATCATATTGCGATAACAGAC
    GGTCAAGAATCTTATACTTATCAAAATTTATACTGTGAAGCGAGTCTATTGGCTAAAAGA
    CTCAAGGCTTATCAACAATCTCGTGTTGGGCTATACATAGATAATTCGATTCAATCGATC
    ATTTTAATACATGCATGTTGGTTGGCAAATATTGAAATTGCGATGATTAATACAAGGTTG
    ACACCTAATGAGATGACGAATCAGATGAAGTCAATCGATGTACAATTGATTTTTTGTACC
    TTGCCACTGGAATTACGAGGGTTTCAAATTGTATCGCTGGATGATATTGAATTCGCTGGA
    AGGGATATTACAACGAACAGTTTGTTGGACAACACAATGGGTATCCAATATGAAACATCG
    AATGAAACTGTGGTGCCGAAAGAGTCGCCGTCCAACATATTAAATACATCATTTAATTTA
    GATGACATTGCATCGATTATGTTTACATCAGGGACAACGGGCCCTCAAAAAGCGGTGCCA
    CAAACGTTTCGTAATCATTATGCCAGTGCAATCGGATGTAAAGAGAGCTTGGGATTTGAT
    CGTGACACTAATTGGCTATCTGTCTTGCCGATTTATCATATTTCGGGTCTCAGTGTACTT
    TTAAGAGCTGTTATTGAAGGTTTTACTGTGCGCATTGTTGATAAATTCAATGCCGAACAA
    ATTTTAACGATGATTAAAAATGAACGAATCACGCACATTTCGCTTGTGCCACAAACTTTA
    AATTGGCTTATGCAACAAGGTTTACATGAACCTTATAATTTACAAAAAATATTACTCGGC
    GGTGCTAAATTATCTGCCACTATGATAGAGACGGCATTACAATATAACCTGCCAATTTAT
    AATTCATTTGGTATGACTGAGACATGTTCGCAATTTTTAACAGCAACACCGGAAATGTTG
    CATGCACGTCCTGACACTGTAGGGATGCCAAGTGCCAATGTTGACGTAAAAATTAAAAAT
    CCTAATAAAGAAGGTCATGGAGAATTAATGATTAAAGGCGCCAATGTGATGAATGTATAT
    TTGTATCCAACAGATTTAACGGGTACGTTTGAAAATGGTTATTTTAATACGGGTGACATT
    GCTGAAATAGATCATGAAGGTTATGTCATGATTTATGACCGACGTAAAGATTTAATTATT
    AGTGGCGGTGAAAATATTTATCCATATCAAATTGAAACGGTAGCGAAGCAATTTCCAGGT
    ATCAGTGACGCAGTATGTGTAGGGCATCCTGATGATACTTGGGGCCAAGTGCCTAAATTA
    TATTTTGTCAGTGAAAGTGACATTTCAAAGGCACAATTGATTGCATATTTATCACAACAT
    TTAGCGAAATATAAAGTACCGAAACACTTTGAAAAGGTTGATACTTTACCTTATACATCA
    ACAGGTAAATTACAAAGAAATAAGTTGTATAGAGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01916
  • symbol: SAOUHSC_01916
  • description: 2-succinylbenzoate-CoA ligase
  • length: 492
  • theoretical pI: 5.90461
  • theoretical MW: 55414.9
  • GRAVY: -0.192276

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.26?  ExPASy
    o-succinylbenzoate--CoA ligase ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 663.8)
    and 11 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 148.4)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 147.2)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 142.1)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 139.3)
    amino acid adenylation domain (TIGR01733; HMM-score: 117.5)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 91.1)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 79.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 58.6)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 30.2)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 16.9)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 14.9)
  • TheSEED  :
    • O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone and Phylloquinone Biosynthesis  O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate  O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 259.4)
    and 2 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 50.1)
    no clan defined DUF4176; Domain of unknown function (DUF4176) (PF13780; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027554
    • TAT(Tat/SPI): 0.000304
    • LIPO(Sec/SPII): 0.002664
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAYQQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLPLELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNLDDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLSVLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQKILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANVDVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIYDRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISKAQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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