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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02374
  • pan locus tag?: SAUPAN005429000
  • symbol: SAOUHSC_02374
  • pan gene symbol?: hmrA
  • synonym:
  • product: aminobenzoyl-glutamate utilization protein B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02374
  • symbol: SAOUHSC_02374
  • product: aminobenzoyl-glutamate utilization protein B
  • replicon: chromosome
  • strand: -
  • coordinates: 2197127..2198311
  • length: 1185
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGTGAAAAACAACAAATTCTCGATTATATAGAAACAAATAAATATAGTTATATCGAA
    ATCAGTCATAGAATTCATGAACGTCCTGAACTTGGTAATGAAGAAATATTTGCGTCTCGA
    ACTTTAATAGATCGTTTGAAAGAGCATGATTTTGAAATTGAAACCGAGATTGCTGGGCAT
    GCAACTGGGTTTATAGCGACATATGATTCAGGACTTGACGGGCCAGCTATAGGTTTTTTA
    GCAGAATACGATGCTTTGCCAGGATTGGGTCATGCTTGTGGTCATAATATCATTGGAACT
    GCAAGTGTTCTTGGTGCAATTGGTTTGAAGCAAGTGATTGACCAAATTGGTGGTAAAGTA
    GTCGTTCTTGGATGTCCAGCTGAAGAAGGTGGGGAAAATGGTAGCGCTAAAGCTTCTTAT
    GTCAAGGCTGGTGTGATTGATCAAATAGACATTGCCTTAATGATTCATCCGGGAAATGAA
    ACTTATAAAACGATTGATACTTTGGCAGTGGATGTTTTAGATGTTAAATTTTACGGAAAA
    AGTGCTCATGCCTCTGAAAATGCAGATGAAGCGTTAAATGCATTAGACGCTATGATTAGT
    TATTTTAATGGTGTAGCACAACTACGACAACATATTAAAAAAGATCAACGTGTGCATGGT
    GTGATTTTAGATGGCGGGAAAGCAGCTAATATTATTCCAGACTATACACATGCTCGTTTT
    TATACTAGAGCAATGACGCGTAAAGAATTGGATATATTAACAGAAAAAGTAAATCAAATC
    GCACGTGGAGCTGCGATACAGACTGGTTGTGATTATGAATTTGGTCGAATTCAAAACGGT
    GTGAATGAATTCATTAAAACGCCGAAATTAGATGATTTATTTGCTAAATATGCTGAAGAA
    GTTGGTGAAGCAGTTATTGATGATGATTTTGGTTATGGCTCTACGGATACAGGGAACGTA
    AGTCATGTTGTGCCAACAATACATCCTCATATTAAAATAGGATCACGTAATTTAGTAGGA
    CATACGCATAGATTTAGAGAAGCGGCTGCGAGTGTACATGGTGATGAAGCATTAATTAAA
    GGCGCTAAAATAATGGCGTTGATGGGGTTAGAGTTAATTACAAATCAAGACGTTTATCAA
    GACATTATTGAAGAGCATGCGCATTTGAAAGGGAATGGGAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02374
  • symbol: SAOUHSC_02374
  • description: aminobenzoyl-glutamate utilization protein B
  • length: 394
  • theoretical pI: 5.27078
  • theoretical MW: 42958.1
  • GRAVY: -0.219289

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 202.1)
    and 5 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 44.7)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 18.7)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 14.9)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 13.7)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 10.7)
  • TheSEED  :
    • amidohydrolase of M40 family
    • HmrA protein involved in methicillin resistance
  • PFAM:
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 31.6)
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 29.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002298
    • TAT(Tat/SPI): 0.000106
    • LIPO(Sec/SPII): 0.0002
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLIDRLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTASVLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNETYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRVHGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGRIQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIGSRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGNGK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02506(rpsC)30S ribosomal protein S3  [3] (data from MRSA252)
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)
    SAOUHSC_01043dihydrolipoamide dehydrogenase  [3] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 3.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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