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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1935 [new locus tag: SA_RS11115 ]
  • pan locus tag?: SAUPAN005429000
  • symbol: hmrA
  • pan gene symbol?: hmrA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1935 [new locus tag: SA_RS11115 ]
  • symbol: hmrA
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2184865..2186049
  • length: 1185
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGTGAAAAACAACAAATTCTCGATTATATAGAAACAAATAAATATAGTTATATCGAA
    ATCAGTCATAGAATTCATGAACGTCCTGAACTTGGTAATGAAGAAATATTTGCGTCTCGA
    ACTTTAATAGATCGTTTGAAAGAGCATGATTTTGAAATTGAAACCGAGATTGCTGGGCAT
    GCAACTGGGTTTATAGCGACATATGATTCAGGTCTTGACGGGCCAGCTATAGGTTTTTTA
    GCAGAATACGATGCTTTGCCAGGGTTGGGTCATGCTTGTGGTCATAATATCATTGGAACT
    GCAAGTGTGCTTGGTGCAATTGGTTTGAAGCAAGTGATTGACCAAATTGGTGGTAAAGTA
    GTCGTTCTTGGATGTCCAGCTGAAGAAGGTGGGGAAAATGGTAGCGCTAAAGCTTCTTAT
    GTCAAGGCTGGTGTGATTGATCAAATAGACATTGCCTTAATGATTCATCCGGGAAATGAA
    ACTTATAAAACGATTGATACTTTGGCAGTGGATGTTTTAGATGTTAAATTTTACGGAAAA
    AGTGCTCATGCCTCTGAAAATGCAGATGAAGCGTTAAATGCATTAGACGCTATGATTAGT
    TATTTTAATGGTGTAGCACAACTACGACAACATATTAAAAAAGATCAACGTGTGCATGGT
    GTGATTTTAGATGGCGGGAAAGCAGCTAATATTATTCCAGACTATACACATGCTCGTTTT
    TATACTAGAGCAATGACGCGTAAAGAATTGGATATATTAACAGAAAAAGTAAATCAAATC
    GCACGTGGAGCTGCGATACAGACTGGTTGTGATTATGAATTTGGTCCAATTCAAAACGGT
    GTGAATGAATTCATTAAAACGCCGAAATTAGATGATTTATTTGCTAAATATGCTGAAGAA
    GTTGGTGAAGCAGTTATTGATGATGATTTTGGTTATGGCTCTACGGATACAGGGAACGTA
    AGTCATGTTGTGCCAACAATACATCCTCATATTAAAATAGGATCACGTAATTTAGTAGGA
    CATACGCATAGATTTAGAGAGGCGGCTGCGAGTGTACATGGTGATGAAGCATTAATTAAA
    GGTGCTAAAATAATGGCGTTGATGGGGTTAGAGTTAATTACAAATCAAGACGTTTATCAA
    GACATTATTGAAGAGCATGCGCATTTGAAAGGGAATGGGAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

⊟Protein[edit | edit source]

Protein Data Bank: 3RAM

⊟General[edit | edit source]

  • locus tag: SA1935 [new locus tag: SA_RS11115 ]
  • symbol: HmrA
  • description: hypothetical protein
  • length: 394
  • theoretical pI: 5.16555
  • theoretical MW: 42899
  • GRAVY: -0.211929

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 201.3)
    and 4 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 45.7)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 18.6)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 14.8)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 13.3)
  • TheSEED  :
    • amidohydrolase of M40 family
    • HmrA protein involved in methicillin resistance
  • PFAM:
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 31.6)
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 28.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002298
    • TAT(Tat/SPI): 0.000106
    • LIPO(Sec/SPII): 0.0002
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLIDRLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTASVLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNETYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRVHGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGPIQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIGSRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGNGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Tiago O Botelho, Tibisay Guevara, Aniebrys Marrero, Pedro Arêde, Viviana S Fluxà, Jean-Louis Reymond, Duarte C Oliveira, F Xavier Gomis-Rüth
Structural and functional analyses reveal that Staphylococcus aureus antibiotic resistance factor HmrA is a zinc-dependent endopeptidase.
J Biol Chem: 2011, 286(29);25697-709
[PubMed:21622555] [WorldCat.org] [DOI] (I p)

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