COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SAP015
• pan locus tag^?:
• symbol: sin
• pan gene symbol^?: —
• synonym:
• product: recombinase Sin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAP015
• symbol: sin
• product: recombinase Sin
• replicon: pN315
• strand: +
• coordinates: 13288..14046
• length: 759
• essential: unknown

⊟Accession numbers

• Gene ID: 1122770 NCBI
• RefSeq: NP_395551 NCBI
• BioCyc:
• MicrobesOnline: 143485 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTAAAAATCAAAATTTCATTGAAACGAAAGAGTATAAACGCTTTGCTGAATTTTGT
  GATGCTTGTATTAAATATCAGTATATTGGTATATGTTATGGTCAACCTGGTGTCGGAAAA
  ACTTTATCGTCTAGGTATTACACAAATTGGGATACTATTGAAAAACAAGTTAACCATAGA
  GGTTGGGAAGATTTAGCTAGTAAAACGACCGATGACATACTATCGGTGGATAAAATATTT
  TATACTGCACCAGCAGAAAAACAATCAGGTAAATCTATAGAAAACAGACCTGTTTTTCAA
  GAAGTATTAAATTTTTTAAGAATGGGAGATCGTTTAATTGTTGAATCTATTGATAGACTA
  GGAAGAAATTACGAGGAAATAATTCAAACAGTTAATTACTTAAAAGATAAAGAAGTTCAG
  CTCATGATAACGAGTTTACCTATGATGAATGAAGTCATTGGAAATCCACTACTAGATAAA
  TTTATGAAAGATTTAATTATTCAAATTTTAGCAATGATTTCAGAACAAGAAAGAAATGAA
  AGTAAACGTCGACAAGCTCAAGGGATTCAAGTCGCCAAAGAAAAAGGTATATATAAAGGG
  AGACCTGTTCTGTATTCTCCCAATGCTAAAGACCCACAAAAGCGTTTAGTTTATTACCGA
  GTTGTTGAATTACTTGAACAGGGTAAATCTATAAGTACTATAGCTAAAGAAGTTGGTATT
  ACACGTCAAACTATATATAGAATAAAAAATAGTAAATAA
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⊟Protein

⊟General

• locus tag: SAP015
• symbol: Sin
• description: recombinase Sin
• length: 252
• theoretical pI: 9.54707
• theoretical MW: 29314.6
• GRAVY: -0.550397

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 16.4)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 16.3)
  probable regulatory domain (TIGR03879; HMM-score: 15.5)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 15.1)
• TheSEED  :
  • ATP-binding protein p271
  • Replication initiation ATPase
  Phages, Prophages, Transposable elements, Plasmids Transposable elements Tn552  ATP-binding protein p271
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 78.1)
  and 26 more
  HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 57.9)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 35.1)
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 31.9)
  CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 27.7)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 24.7)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 23.9)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 23.8)
  HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 19.5)
  DUF433; Protein of unknown function (DUF433) (PF04255; HMM-score: 18.8)
  no clan defined DUF2802; Protein of unknown function (DUF2802) (PF10975; HMM-score: 18.2)
  HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 18.1)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 17.5)
  HTH_11; HTH domain (PF08279; HMM-score: 15.5)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14)
  HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 13.9)
  HTH_22; HTH domain (PF13309; HMM-score: 13.8)
  Trp_repressor; Trp repressor protein (PF01371; HMM-score: 13.7)
  HTH_Tnp_1; Transposase (PF01527; HMM-score: 13.5)
  P-loop_NTPase (CL0023) RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 13.4)
  HTH (CL0123) Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 13.1)
  TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.8)
  GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 12.3)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.2)
  no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 12)
  DUF3572; Protein of unknown function (DUF3572) (PF12096; HMM-score: 11.7)
  HTH (CL0123) Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.165406
  • TAT(Tat/SPI): 0.001391
  • LIPO(Sec/SPII): 0.010809
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 16119215 NCBI
• RefSeq: NP_395551 NCBI
• UniProt: A0A0H3JP38 UniProt

⊟Protein sequence

• MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSRYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYTAPAEKQSGKSIENRPVFQEVLNFLRMGDRLIVESIDRLGRNYEEIIQTVNYLKDKEVQLMITSLPMMNEVIGNPLLDKFMKDLIIQILAMISEQERNESKRRQAQGIQVAKEKGIYKGRPVLYSPNAKDPQKRLVYYRVVELLEQGKSISTIAKEVGITRQTIYRIKNSK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: binL > tnp > sin

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications