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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1537 [new locus tag: SAUSA300_RS08380 ]
  • pan locus tag?: SAUPAN004160000
  • symbol: SAUSA300_1537
  • pan gene symbol?: rsmE
  • synonym:
  • product: 16S ribosomal RNA methyltransferase RsmE

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1537 [new locus tag: SAUSA300_RS08380 ]
  • symbol: SAUSA300_1537
  • product: 16S ribosomal RNA methyltransferase RsmE
  • replicon: chromosome
  • strand: -
  • coordinates: 1686440..1687192
  • length: 753
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGCAACGTTATTTCATAGACCAAAACGCTGATGTAAGTCAGCGTTTTTTTATTACAAAA
    AAAGAAGATATTCATCATATAACAAATGTAATGAGATATGATGTTGGTAGTAAAATTATT
    TTAACTTTTAATGATCAAACAGTTTATTTATGTGAAATTGCTGACATAACAAGTGATAAA
    ATTGAAGTTGATTTAAAAGAAAAACAAAATATTAATACAGAATTGCCAGTTGATGTTACG
    ATTTGCAGTGGACTAATCAAAGCTGACAAATATGAGTGGTTGCTACAAAAAGCTACTGAA
    TTGGGTGCTTCATCATTTATAGCTGTGAGCATGGAACGTTCAATTGTTAAATTAAACGAG
    GCAAAAGTTGCCAAAAAGATAGAGCGTTGGCAAAAAATAATTAAAGAAGCTGCTGAACAA
    AGCTATCGTTTAGTGATTCCATCTATTCAATTCGAGTCGAATTTAAAATTAATTTGTGAT
    ACTATTGATAATTATGACTATATTCTTATTGCATATGAAGAGGAAGCAAAAGATGGTGAG
    TTAAGCAATTTCAAGCAAACTTTACAACAATTCAATGCTCAGGATAAAGTGTTGATGATT
    TTTGGTCCTGAGGGTGGTTTGTCAGAAAATGAAATTTCATTATTTAGTAATACTAGTACA
    GTTGTTGGTCTTGGGCCGAGAATTTTACGGGCAGAAACTGCGCCATTGTATGCGTTAAGT
    GCAATAAGTTATGAAAAAGAATTAATGGGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1537 [new locus tag: SAUSA300_RS08380 ]
  • symbol: SAUSA300_1537
  • description: 16S ribosomal RNA methyltransferase RsmE
  • length: 250
  • theoretical pI: 4.60587
  • theoretical MW: 28469.4
  • GRAVY: -0.1916

Function[edit | edit source]

  • reaction:
    EC 2.1.1.193?  ExPASy
    16S rRNA (uracil1498-N3)-methyltransferase S-adenosyl-L-methionine + uracil1498 in 16S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, RsmE family (TIGR00046; EC 2.1.1.-; HMM-score: 204.7)
  • TheSEED  :
    • Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)
  • PFAM:
    SPOUT (CL0098) Methyltrans_RNA; RNA methyltransferase (PF04452; HMM-score: 247.9)
    and 2 more
    SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 16.1)
    bBprotInhib (CL0354) Staphostatin_A; Staphostatin A (PF09022; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001491
    • TAT(Tat/SPI): 0.000117
    • LIPO(Sec/SPII): 0.000288
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLYALSAISYEKELMG

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)

Relevant publications[edit | edit source]