PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2254 [new locus tag: SAUSA300_RS12450 ]
• pan locus tag^?: SAUPAN005775000
• symbol: SAUSA300_2254
• pan gene symbol^?: —
• synonym:
• product: glycerate dehydrogenase-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2254 [new locus tag: SAUSA300_RS12450 ]
• symbol: SAUSA300_2254
• product: glycerate dehydrogenase-like protein
• replicon: chromosome
• strand: +
• coordinates: 2420781..2421734
• length: 954
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913099 NCBI
• RefSeq: YP_494889 NCBI
• BioCyc: GH3C-2241 BioCyc
• MicrobesOnline: 1293769 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGAAAAAGTTTATGTTGCTGGTGCAATACCAGAAGTAGGTTTAAAACTTTTACAAGAA
  CATTTTGAAGTTGAAATGTATGAAGGTAAAGGATTAGTCGATAAAGACACTTTAATTAAA
  GGTGTTAAAAACGCGACTGCCTTAATTAGTTTATTATCTACAAACGTTGATAAAGATGTT
  ATCGATGCTGGTAAAGACTTAAAAATCATTGCCAACTATGGCGCTGGTTTTAATAATATT
  GATATCGAGTATGCCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACA
  AACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAA
  GGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGT
  AGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTA
  GCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATCGCAAAGAA
  GAAAAAGAACGAGAAATCGGTGCAAAATATGTAGATTTAGATACACTATTAAAGAATGCA
  GATTTTATCACTATCAACGCTGCTTATAATCCTAAAATGCATCATTTAATTGATACAGAA
  CAATTTAAAATGATGAAATCTACGGCGTATTTAATCAATGCCTCTCGTGGTCCAATCGTG
  CACGAACAAGCACTAGTGCAAGCATTGAAAGATAATGAAATTGAAGGTGCTGCACTTGAT
  GTATATGAATTTGAACCAGATATTACCGATGACTTAAAATCACTTAATAATGTAGTATTA
  ACACCGCATATTGGTAATGCTACATTTGAAGCACGTGACATGATGTCTAAAATTGTTGCT
  AATGCTGCAATTAGTGCCGTTCAAGGTGAGAAACCACAATTTGTCGTTAACTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2254 [new locus tag: SAUSA300_RS12450 ]
• symbol: SAUSA300_2254
• description: glycerate dehydrogenase-like protein
• length: 317
• theoretical pI: 4.91492
• theoretical MW: 34674.3
• GRAVY: -0.127445

⊟Function

• reaction:
  EC 1.1.1.-?  ExPASy
• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 268)
  and 2 more
  Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 17.5)
  Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 12.1)
• TheSEED  :
  • Glyoxylate reductase (EC 1.1.1.26)
  • Glyoxylate reductase (EC 1.1.1.79)
  • Hydroxypyruvate reductase (EC 1.1.1.81)
  Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.26)
  and 3 more
  Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.79)
  Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Hydroxypyruvate reductase (EC 1.1.1.81)
  Carbohydrates Organic acids Glycerate metabolism  Hydroxypyruvate reductase (EC 1.1.1.81)
• PFAM:
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 203)
  and 5 more
  Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 113.3)
  NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 21)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 19.4)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 18.3)
  AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001313
  • TAT(Tat/SPI): 0.000138
  • LIPO(Sec/SPII): 0.000235
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162105 NCBI
• RefSeq: YP_494889 NCBI
• UniProt: Q2FEI9 UniProt

⊟Protein sequence

• MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications