PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00105 [old locus tag: SAUSA300_0020 ]
• pan locus tag^?: SAUPAN000032000
• symbol: SAUSA300_RS00105
• pan gene symbol^?: walR
• synonym:
• product: DNA-binding response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00105 [old locus tag: SAUSA300_0020 ]
• symbol: SAUSA300_RS00105
• product: DNA-binding response regulator
• replicon: chromosome
• strand: +
• coordinates: 24952..25653
• length: 702
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (24952..25653) NCBI
• BioCyc: see SAUSA300_0020
• MicrobesOnline: see SAUSA300_0020

⊟Phenotype

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⊟DNA sequence

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  ATGGCTAGAAAAGTTGTTGTAGTTGATGATGAAAAACCGATTGCTGATATTTTAGAATTT
  AACTTAAAAAAAGAAGGATACGATGTGTACTGTGCATACGATGGTAATGATGCAGTCGAC
  TTAATTTATGAAGAAGAACCAGACATCGTATTACTAGATATCATGTTACCTGGTCGTGAT
  GGTATGGAAGTATGTCGTGAAGTGCGCAAAAAATACGAAATGCCAATAATAATGCTTACT
  GCTAAAGATTCAGAAATTGATAAAGTGCTTGGTTTAGAACTAGGTGCAGATGACTATGTA
  ACGAAACCGTTTAGTACGCGTGAATTAATCGCACGTGTGAAAGCGAACTTACGTCGTCAT
  TACTCACAACCAGCACAAGACACTGGAAATGTAACGAATGAAATCACAATTAAAGATATT
  GTGATTTATCCAGACGCATATTCTATTAAAAAACGTGGCGAAGATATTGAATTAACACAT
  CGTGAATTTGAATTGTTCCATTATTTATCAAAACATATGGGACAAGTAATGACACGTGAA
  CATTTATTACAAACAGTATGGGGCTATGATTACTTTGGCGATGTACGTACGGTCGATGTA
  ACGATTCGTCGTTTACGTGAAAAGATTGAAGATGATCCGTCACATCCTGAATATATTGTG
  ACGCGTAGAGGCGTTGGATATTTCCTCCAACAACATGAGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_RS00105 [old locus tag: SAUSA300_0020 ]
• symbol: SAUSA300_RS00105
• description: DNA-binding response regulator
• length: 233
• theoretical pI: 4.81211
• theoretical MW: 27191.8
• GRAVY: -0.475536

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 235.8)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 235.8)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 191.9)
  and 7 more
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 132.5)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 70.6)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 70.6)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 70.6)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 69.4)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 41.1)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 40.3)
• TheSEED: see SAUSA300_0020
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 103.1)
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 97)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by WalR*, response regulator important in cell wall homeostasis: in NCTC8325

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004145
  • TAT(Tat/SPI): 0.000213
  • LIPO(Sec/SPII): 0.000302
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446024121 NCBI
• RefSeq: WP_000101976 NCBI
• UniProt: see SAUSA300_0020

⊟Protein sequence

• MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYEEEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTKPFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTHREFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEYIVTRRGVGYFLQQHE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

  SAUSA300_RS02340		ABC transporter substrate-binding protein   (data from MRSA252)
  SAUSA300_RS02635		cysteine synthase   (data from MRSA252)
  SAUSA300_RS05360		dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex   (data from MRSA252)
  SAUSA300_RS06220		30S ribosomal protein S2   (data from MRSA252)
  SAUSA300_RS07445		30S ribosomal protein S1   (data from MRSA252)
  SAUSA300_RS11380		uracil phosphoribosyltransferase   (data from MRSA252)
  SAUSA300_RS12120		30S ribosomal protein S3   (data from MRSA252)
  SAUSA300_RS14105		malate:quinone oxidoreductase   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Rex see SAUSA300_0020

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications