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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS07445 [old locus tag: SAUSA300_1365 ]
- pan locus tag?: SAUPAN003937000
- symbol: SAUSA300_RS07445
- pan gene symbol?: rpsA
- synonym:
- product: 30S ribosomal protein S1
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS07445 [old locus tag: SAUSA300_1365 ]
- symbol: SAUSA300_RS07445
- product: 30S ribosomal protein S1
- replicon: chromosome
- strand: -
- coordinates: 1531972..1533147
- length: 1176
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1531972..1533147) NCBI
- BioCyc: SAUSA300_RS07445 BioCyc
- MicrobesOnline: see SAUSA300_1365
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGACTGAAGAATTCAATGAATCAATGATTAACGATATTAAAGAAGGTGACAAAGTCACT
GGCGAGGTACAACAAGTTGAAGACAAGCAAGTTGTTGTTCATATCAACGGTGGTAAATTT
AATGGGATTATTCCTATTAGTCAATTATCTACGCATCATATTGATAGCCCAAGTGAAGTT
GTAAAAGAGGGCGACGAAGTTGAAGCATATGTCACTAAAGTTGAGTTTGATGAAGAAAAT
GAAACTGGAGCTTACATCTTATCTAGAAGACAACTTGAAACTGAGAAGTCTTATAGTTAT
TTACAAGAAAAATTAGATAATAATGAAATCATCGAAGCGAAAGTAACAGAAGTAGTTAAA
GGTGGTTTGGTTGTTGATGTAGGACAAAGAGGTTTTGTTCCGGCTTCACTAATTTCAACA
GACTTCATTGAGGATTTCTCTGTGTTTGATGGACAAACAATTCGTATTAAAGTTGAAGAA
TTGGATCCTGAAAATAATAGAGTCATTTTAAGCCGTAAAGCAGTTGAACAAGAAGAAAAC
GATGCTAAAAAAGATCAATTATTACAATCTTTAAATGAAGGCGATGTTATTGATGGTAAA
GTAGCGCGTTTAACTCAATTTGGTGCATTTATAGACATTGGCGGTGTTGATGGTTTAGTG
CATGTATCTGAACTTTCTCACGAACATGTTCAAACACCAGAAGAAGTAGTTTCAATTGGT
CAAGATGTTAAAGTTAAAATTAAATCTATTGATAGAGATACAGAACGTATTTCATTATCA
ATCAAAGATACGTTACCAACACCTTTCGAAAATATTAAAGGTCAATTCCACGAAAATGAT
GTCATTGAAGGTGTCGTAGTAAGATTGGCAAACTTTGGTGCATTTGTTGAAATTGCACCA
GGTGTACAAGGACTTGTACATATTTCTGAAATTGCACACAAACACATTGGTACGCCAGGT
GAAGTGTTAGAACCTGGTCAACAAGTAAATGTTAAAATATTAGGTATTGATGAAGAGAAT
GAAAGAGTATCACTATCTATTAAAGCAACATTACCAAACGAAGATGTTGTTGAAAGTGAT
CCTTCTACGACTAAGGCGTACTTAGAAAACGAAGAAGAAGATAATCCAACAATTGGCGAT
ATGATTGGTGATAAACTTAAAAATCTTAAACTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS07445 [old locus tag: SAUSA300_1365 ]
- symbol: SAUSA300_RS07445
- description: 30S ribosomal protein S1
- length: 391
- theoretical pI: 4.23557
- theoretical MW: 43287
- GRAVY: -0.390537
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bS1 (TIGR00717; HMM-score: 286.5)and 8 moreTranscription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 147.8)guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 58.7)Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM-score: 44.2)Transcription Degradation of RNA ribonuclease R (TIGR02063; EC 3.1.-.-; HMM-score: 36.7)MAST domain (TIGR04204; HMM-score: 14.1)Energy metabolism Glycolysis/gluconeogenesis carbon storage regulator (TIGR00202; HMM-score: 9.8)Regulatory functions RNA interactions carbon storage regulator (TIGR00202; HMM-score: 9.8)Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 8.5)
- TheSEED: see SAUSA300_1365
- PFAM: OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 252.3)and 8 moreCusF_Ec; Copper binding periplasmic protein CusF (PF11604; HMM-score: 16.6)Cdc48_2-like (CL0402) CDC48_2; Cell division protein 48 (CDC48), domain 2 (PF02933; HMM-score: 15.9)OB (CL0021) S1_2; S1 domain (PF13509; HMM-score: 15.7)EXOSC1; Exosome component EXOSC1/CSL4 (PF10447; HMM-score: 12.4)RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 10.5)no clan defined DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 9.9)CsrA; Global regulator protein family (PF02599; HMM-score: 7.9)KOW (CL0107) KOW; KOW motif (PF00467; HMM-score: 7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.021822
- TAT(Tat/SPI): 0.000535
- LIPO(Sec/SPII): 0.000886
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446056099 NCBI
- RefSeq: WP_000133954 NCBI
- UniProt: see SAUSA300_1365
⊟Protein sequence[edit | edit source]
- MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGIIPISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQEKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEELDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDGLVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQFHENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKILGIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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