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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02870 [old locus tag: SAUSA300_0537 ]
  • pan locus tag?: SAUPAN002324000
  • symbol: SAUSA300_RS02870
  • pan gene symbol?: araB
  • synonym:
  • product: ribulokinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02870 [old locus tag: SAUSA300_0537 ]
  • symbol: SAUSA300_RS02870
  • product: ribulokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 602028..603665
  • length: 1638
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTATAGCATTGGAATTGATTATGGAACTGCTTCAGGCCGTGTGTTTTTAATTAAT
    ACAACTAACGGTCAAGTAGTATCAAAATTTGTGAAACCATATACACATGGTGTCATTGAG
    AGTGAATTAAATGGTTTGAAAATACCACATACATATGCACTTCAAAATAGTAATGATTAT
    TTAGAAATTATGGAAGAAGGAATATCATATATAGTACGTGAATCAAAAATAGATCCAGAC
    AATATAGTAGGTATTGGTATAGACTTTACTTCATCTACTATTATTTTTACTGACGAAAAC
    CTTAACCCGGTACATAACTTAAAACAATTTAAAAACAATCCACATGCGTATGTGAAACTT
    TGGAAACATCATGGTGCATATAAAGAGGCAGAGAAATTATATCAAACTGCTATTGAAAAT
    AATAATAAGTGGTTAGGCCATTATGGATATAATGTTAGTAGTGAATGGATGATTCCCAAA
    ATAATGGAGGTCATGAATCGAGCACCAGAAATTATGGAAAAAACGGCTTATATTATGGAA
    GCGGGCGATTGGATTGTAAATAAATTAACTAATAAAAATGTACGCTCGAATTGTGGATTA
    GGTTTCAAAGCATTTTGGGAAGAAGAAACAGGGTTTCATTATGATTTATTTGATAAAATA
    GACCCCAAATTATCAAAAGTAATTCAAGATAAAGTATCTGCACCGGTTGTTAATATTGGT
    GAAGCAGTAGGGAAACTGGATGATAAAATGGCACAGAAATTAGGATTATCAAAAGAAACT
    ATGGTAAGTCCTTTTATTATTGATGCCCATGCTAGTTTATTAGGTATTGGGTCTGAAAAA
    GATAAAGAAATGACTATGGTGATGGGAACAAGCACATGCCATCTTATGTTAAATGAAAAG
    CAACATCAAGTGCCAGGTATATCAGGTTCTGTAAAAGGAGCAATTATTCCAGAATTATTT
    GCTTATGAAGCGGGGCAATCAGCAGTAGGTGATTTGTTTGAGTATGTCGCTAAGCAAGCA
    CCAAAGTCATATGTAGATGAAGCAGAAAATAGAAATATGACTGTATTTGAATTAATGAAT
    GAAAAGATAAAACATCAAATGCCAGGTGAAAGTGGGCTCATTGCTCTTGATTGGCATAAT
    GGAAATCGAAGTGTATTAAGTGATAGCAATTTAACAGGTTGTATCTTTGGATTAACTTTA
    CAAACTAAGCATGAGGATATTTATAGAGCATATTTAGAAGCTACAGCATTTGGTACTAAG
    ATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGC
    GGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAA
    CTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTC
    AGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTA
    TACGAAATTAATCCAGAAGCGGAAAAAGTACAAAGGTATGAAACATTATTTAAAGCTTAT
    AAGGCTTTACATGATATCCATGGTTATAAAAAAGCTAATATAATGAAAGATATCCAGAGT
    TTAAGAGTTGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1638

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS02870 [old locus tag: SAUSA300_0537 ]
  • symbol: SAUSA300_RS02870
  • description: ribulokinase
  • length: 545
  • theoretical pI: 6.04212
  • theoretical MW: 61010.4
  • GRAVY: -0.278165

Function[edit | edit source]

  • reaction:
    EC 2.7.1.16?  ExPASy
    Ribulokinase ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 557)
    and 5 more
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 209.7)
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 154.3)
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 86.9)
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 68.4)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 58.6)
  • TheSEED: see SAUSA300_0537
  • PFAM:
    Actin_ATPase (CL0108) FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 126)
    and 4 more
    FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 63.3)
    no clan defined DUF4846; Domain of unknown function (4846) (PF16138; HMM-score: 17.5)
    Actin_ATPase (CL0108) BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 13.3)
    Concanavalin (CL0004) SPRY; SPRY domain (PF00622; HMM-score: 13)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.64
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013869
    • TAT(Tat/SPI): 0.000535
    • LIPO(Sec/SPII): 0.004046
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELNGLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLNPVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPKIMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]