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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS03540 [old locus tag: SAUSA300_0659 ]
  • pan locus tag?: SAUPAN002556000
  • symbol: SAUSA300_RS03540
  • pan gene symbol?:
  • synonym:
  • product: MFS transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS03540 [old locus tag: SAUSA300_0659 ]
  • symbol: SAUSA300_RS03540
  • product: MFS transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 735243..736463
  • length: 1221
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATATGTTTGCAGCGTTATTACAAATAAAGAATTATAAACTCTTTGTTGCTAATATG
    TTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAA
    GATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAG
    TTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATT
    ATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGAT
    ACAATCTGGTTATTCATATTACTATATGCGATTTTCCAAGGATTATTTGCACCAGCAATG
    CCCCAACTTTACGCATCTGCTAGAGAATCTATCAATGTTTCAAGCTCTAAAGATAGAGCT
    CAATTTGCCAACACAGTATTACGTTCAATGTTCTCATTGGGCTTTTTATTTGGTCCATTT
    ATTGGTGCCCAATTAATCGGATTAAAAGGCTATGCTGGATTGTTTGGTGGAACAATAAGT
    ATCATTTTATTTACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACAC
    CCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACG
    CTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATG
    AATATGCCTTTATTTGTTACTGATTATTTAAAAGAAAATGAACAACATGTCGGTTATTTA
    GCTAGTTTATGTGCTGGTTTAGAAGTGCCATTTATGATCATTCTTGGCGTTTTATCATCT
    AGATTACAGACTCGAACATTGTTGATTTATGGAGCGATTTTTGGTGGTTTATTCTACTTC
    AGCATTGGGGTATTTAAAAACTTCTATATGATGTTAGCAGGACAGGTGTTTTTAGCTATT
    TTCTTAGCGGTTCTTTTAGGAATTGGTATTAGTTATTTCCAAGATATCTTACCAGATTTT
    CCAGGATACGCTTCAACACTATTTTCTAATGCAATGGTTATTGGACAGTTAGGCGGTAAC
    CTATTAGGTGGTGCTATGAGTCACTGGGTAGGTTTGGAAAATGTATTTTTTGTATCAGCA
    GCATCAATCATGTTAGGTATGATACTTATATTCTTTACTAAAAATCAAAAAATTACAAAA
    GAGGATGTGATATCAACATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1221

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS03540 [old locus tag: SAUSA300_0659 ]
  • symbol: SAUSA300_RS03540
  • description: MFS transporter
  • length: 406
  • theoretical pI: 9.39681
  • theoretical MW: 45130.5
  • GRAVY: 0.813054

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 161.2)
    and 5 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 44.8)
    multidrug resistance protein (TIGR00880; HMM-score: 41.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 17.1)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
  • TheSEED: see SAUSA300_0659
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 120.4)
    and 3 more
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 29.5)
    ATG22; Vacuole effluxer Atg22 like (PF11700; HMM-score: 17.5)
    no clan defined Ilm1; Increased loss of mitochondrial DNA protein 1 (PF10311; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016845
    • TAT(Tat/SPI): 0.00127
    • LIPO(Sec/SPII): 0.006278
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGMTTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTIWLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPFIGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKDKTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILGVLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFFTKNQKITKEDVIST

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]