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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_pUSA030005 [new locus tag: SAUSA300_RS14760 ]
  • pan locus tag?:
  • symbol: SAUSA300_pUSA030005
  • pan gene symbol?:
  • synonym:
  • product: transposase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_pUSA030005 [new locus tag: SAUSA300_RS14760 ]
  • symbol: SAUSA300_pUSA030005
  • product: transposase
  • replicon: pUSA03
  • strand: +
  • coordinates: 5734..6408
  • length: 675
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTAC
    TATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGGACGTGGTGTA
    AACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAA
    ATTTGGAAGAAAAAGCATAAAAAAGCTTATTACAAATGGCGTATTGATGAGACGTACATC
    AAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTGATGCAGAGGGACATACATTA
    GATATTTGGTTGCGTAAGCAACGAGATAATCATTCAGCATATGCGTTTATTAAACGTCTC
    ATTAAACAATTTGGTAAACCTCAAAAGGTAATTACAGATCAGGCACCTTCAACGAAGGTA
    GCAATGGCTAAAGTAATTAAAGCTTTTAAACTTAAACCTGACTGCCATTGTACATCGAAA
    TATCTGAATAACCTCATTGAGCAAGATCACCGTCATATTAAAGTAAGAAAGACAAGGTAT
    CAAAGTATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATAT
    AAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATC
    ATGCTAGCAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_pUSA030005 [new locus tag: SAUSA300_RS14760 ]
  • symbol: SAUSA300_pUSA030005
  • description: transposase
  • length: 224
  • theoretical pI: 10.4686
  • theoretical MW: 26813.1
  • GRAVY: -0.576339

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Transposase for insertion sequence-like element IS431mec
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_IS240; DDE domain (PF13610; HMM-score: 133.2)
    and 9 more
    rve; Integrase core domain (PF00665; HMM-score: 54.7)
    DDE_Tnp_IS66; Transposase IS66 family (PF03050; HMM-score: 18)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.6)
    HTH_32; Homeodomain-like domain (PF13565; HMM-score: 17.1)
    RNase_H (CL0219) DDE_3; DDE superfamily endonuclease (PF13358; HMM-score: 16.7)
    HTH (CL0123) HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 14.2)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 13.9)
    no clan defined Melibiase_2_C; Alpha galactosidase A C-terminal beta sandwich domain (PF17450; HMM-score: 12.9)
    HEM4; Uroporphyrinogen-III synthase HemD (PF02602; HMM-score: 11.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007803
    • TAT(Tat/SPI): 0.000561
    • LIPO(Sec/SPII): 0.002874
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHHSTVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]