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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12475 [old locus tag: SA2173 ]
  • pan locus tag?: SAUPAN005912000
  • symbol: SA_RS12475
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12475 [old locus tag: SA2173 ]
  • symbol: SA_RS12475
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2442332..2442697
  • length: 366
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2442332..2442697) NCBI
  • BioCyc: SA_RS12475 BioCyc
  • MicrobesOnline: see SA2173

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAAGAAGAAAAAAAACGCGATTAAAGTTATCATTATATTATTTATTTCATTAATTGTT
    GCAGTAGCATTTTTCTTTGGTCTGAAAACATATCAAGGCCATAAGAATATTCAACTAATT
    GACAGTTACTTGGAAGAGAAAAATCTAAAAGATAAAATTAAATCAGAAAAAACAGAGTAT
    AGTGCTAAAAAAGGTCTCTTCTATAAGGAAGTTACGTTTAAAGATGAACCTGGTGTAACT
    TATGTTGTGCAACCAATCAGTACTAATAAAGGATTATTTGTTGAAGGCTTTGATACAGAG
    ACTAAGAAAAGTTTGAAAACTGCAAAGCATAAATATTTCAATCAAAATTATAAACCATCA
    AAATAG
    60
    120
    180
    240
    300
    360
    366

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS12475 [old locus tag: SA2173 ]
  • symbol: SA_RS12475
  • description: hypothetical protein
  • length: 121
  • theoretical pI: 10.3674
  • theoretical MW: 14007.3
  • GRAVY: -0.459504

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA2173
  • PFAM:
    no clan defined DUF3139; Protein of unknown function (DUF3139) (PF11337; HMM-score: 71.6)
    and 4 more
    O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 18.5)
    no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 16)
    O-anti_assembly (CL0499) O-antigen_lig; O-antigen ligase like membrane protein (PF13425; HMM-score: 13.9)
    no clan defined DUF2648; Protein of unknown function (DUF2648) (PF10855; HMM-score: 13.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.230821
    • TAT(Tat/SPI): 0.000613
    • LIPO(Sec/SPII): 0.042263
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYLEEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETKKSLKTAKHKYFNQNYKPSK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]