NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02254
- pan locus tag?: SAUPAN005241000
- symbol: groEL
- pan gene symbol?: groEL
- synonym:
- product: chaperonin GroEL
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02254
- symbol: groEL
- product: chaperonin GroEL
- replicon: chromosome
- strand: -
- coordinates: 2086760..2088376
- length: 1617
- essential: yes [1] DEG other strains
- comment: The sequence of SAOUHSC_02254 was corrected based on the resequencing performed by Berscheid et al., 2012 [2].
⊟Accession numbers[edit | edit source]
- Gene ID: 3919674 NCBI
- RefSeq: YP_500736 NCBI
- BioCyc: G1I0R-2126 BioCyc
- MicrobesOnline: 1290692 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1561ATGGTTAAACAATTGAAATTCTCTGAAGATGCACGTCAAGCAATGTTACGTGGTGTTGAC
CAACTTGCAAATGCAGTTAAAGTAACGATTGGTCCTAAAGGACGTAATGTTGTATTAGAT
AAAGAGTTTACAGCACCTTTAATTACGAATGATGGTGTGACGATTGCTAAAGAAATCGAA
TTAGAAGATCCATATGAAAATATGGGGGCTAAACTAGTTCAAGAAGTCGCAAATAAGACA
AATGAAATTGCTGGTGACGGTACGACAACTGCAACAGTATTAGCTCAAGCAATGATTCAA
GAAGGCTTGAAAAATGTTACAAGTGGTGCGAACCCAGTTGGTTTACGACAAGGTATCGAC
AAAGCAGTTAAAGTTGCTGTTGAAGCGTTACATGAAAATTCTCAAAAAGTTGAAAATAAA
AATGAAATTGCGCAAGTAGGTGCGATTTCAGCAGCAGATGAAGAAATTGGACGTTATATT
TCTGAAGCTATGGAAAAAGTAGGTAACGATGGTGTCATTACAATTGAAGAATCAAATGGA
CTAAACACTGAACTAGAAGTGGTTGAAGGTATGCAATTTGATCGTGGTTATCAATCACCG
TATATGGTTACTGATTCAGATAAAATGGTTGCTGAATTAGAACGCCCATACATTTTAGTA
ACAGATAAGAAAATCTCGTCTTTCCAAGATATCTTACCTTTATTAGAACAAGTGGTTCAA
TCTAATCGTCCAATCTTAATTGTAGCTGATGAAGTTGAAGGCGATGCATTAACAAATATC
GTGCTAAACCGTATGCGTGGCACATTTACAGCTGTTGCAGTAAAAGCACCTGGTTTTGGT
GATCGTAGAAAAGCGATGCTTGAAGATTTAGCTATTTTAACTGGTGCGCAAGTGATTACT
GATGATTTAGGCTTAGATTTAAAAGATGCATCAATTGATATGTTAGGTACTGCAAGTAAA
GTAGAAGTAACTAAAGATAATACCACTGTTGTTGATGGTGACGGTGACGAAAACAGCATT
GATGCACGTGTTAGCCAATTGAAATCTCAAATTGAAGAAACTGAATCTGACTTTGATCGT
GAAAAATTACAAGAGCGCTTAGCTAAATTAGCAGGTGGTGTTGCAGTTATCAAAGTAGGT
GCAGCAAGTGAAACAGAGCTTAAAGAACGTAAATTACGTATTGAAGATGCATTAAATTCT
ACACGTGCAGCAGTTGAAGAAGGTATTGTTGCAGGTGGTGGTACTGCATTAGTAAATGTT
TACCAAAAAGTAAGTGAAATTGAAGCTGAAGGTGACATTGAAACAGGTGTAAATATTGTA
CTTAAAGCATTAACTGCACCAGTTCGTCAAATTGCTGAAAATGCAGGATTAGAAGGTTCT
GTTATTGTAGAACGTTTGAAAAACGCAGAGCCGGGTGTTGGTTTTAACGCTGCTACAAAC
GAGTGGGTTAATATGTTAGAAGAAGGTATCGTTGATCCAACTAAAGTAACACGCTCAGCA
TTACAACATGCTGCAAGTGTTGCAGCAATGTTCTTAACGACTGAAGCGGTTGTAGCATCA
ATTCCAGAAAAAAATAATGACCAACCTAACATGGGTGGCATGCCAGGAATGATGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02254
- symbol: GroEL
- description: chaperonin GroEL
- length: 538
- theoretical pI: 4.27242
- theoretical MW: 57629.9
- GRAVY: -0.176952
⊟Function[edit | edit source]
- ⊞TIGRFAM: Protein fate Protein folding and stabilization chaperonin GroL (TIGR02348; HMM-score: 823.7)and 10 more
- ⊞TheSEED :
- Heat shock protein 60 family chaperone GroEL
Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI Heat shock protein 60 family chaperone GroELand 1 more - PFAM: no clan defined Cpn60_TCP1; TCP-1/cpn60 chaperonin family (PF00118; HMM-score: 334.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTAPLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVAAMFLTTEAVVASIPEKNNDQPNMGGMPGMM
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [3] [4]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- ⊟interaction partners:
SAOUHSC_00530 elongation factor Tu [5] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [6] ⊟Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
Int J Med Microbiol: 2012, 302(2);84-7
[PubMed:22417616] [WorldCat.org] [DOI] (I p) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ Jump up to: 6.0 6.1 6.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)