NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0861 [new locus tag: SAUSA300_RS04645 ]
- pan locus tag?: SAUPAN003060000
- symbol: gudB
- pan gene symbol?: gudB
- synonym:
- product: NAD-specific glutamate dehydrogenase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0861 [new locus tag: SAUSA300_RS04645 ]
- symbol: gudB
- product: NAD-specific glutamate dehydrogenase
- replicon: chromosome
- strand: +
- coordinates: 939154..940398
- length: 1245
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914066 NCBI
- RefSeq: YP_493561 NCBI
- BioCyc: see SAUSA300_RS04645
- MicrobesOnline: 1292376 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201ATGACTGAGAACAATAATTTAGTAACTTCTACTCAAGGAATTATTAAAGAAGCATTGCAT
AAATTGGGATTTGACGAAGGAATGTACGATTTAATTAAAGAACCTTTAAGAATGTTACAA
GTGCGTATCCCTGTACGAATGGATGATGGCACAGTAAAAACATTCACAGGTTACCGTGCG
CAACATAATGATGCTGTTGGACCAACAAAAGGGGGCGTGCGTTTCCACCCAGATGTTGAT
GAAGAAGAAGTAAAAGCATTATCAATGTGGATGACTTTGAAATGTGGCATTGTAAACTTA
CCATACGGTGGTGGTAAGGGTGGTATCGTTTGTGATCCACGTCAAATGAGCATTCATGAA
GTTGAACGTTTATCACGCGGATATGTAAGAGCAATTTCACAATTCGTAGGTCCGAACAAA
GATATTCCAGCACCAGATGTATTTACAAACTCACAAATTATGGCTTGGATGATGGATGAA
TATAGTGCATTAGATAAATTTAATTCACCAGGTTTCATCACAGGTAAACCAATTGTATTG
GGTGGTTCTCATGGACGCGACAGATCAACTGCACTAGGTGTAGTTATTGCAATTGAACAA
GCTGCAAAACGTCGTAATATGCAAATTGAAGGTGCCAAGGTTGTTATTCAAGGTTTCGGT
AATGCCGGAAGTTTCTTAGCTAAATTCTTATATGATTTAGGTGCAAAAATTGTAGGTATC
TCTGATGCTTACGGTGCATTACACGATCCAAATGGCTTAGATATAGATTATTTATTAGAC
CGTCGTGATAGTTTTGGTACGGTAACAAATTTATTTGAAGAAACAATCTCAAATAAAGAA
TTGTTTGAATTAGATTGTGACATTTTAGTACCAGCGGCTATTTCAAACCAAATTACAGAA
GACAATGCACATGATATTAAAGCTAGTATCGTTGTTGAAGCTGCTAATGGACCTACAACA
CCAGAAGCAACACGTATTTTAACTGAACGTGGTATATTATTAGTTCCAGACGTATTAGCA
AGTGCTGGTGGTGTAACGGTTTCTTACTTCGAATGGGTACAAAATAATCAAGGTTATTAT
TGGTCTGAAGAAGAAGTTGATGAAAAGCTACGTGAAAAATTAGAAGCGGCATTTGATACG
ATTTACGAATTGTCTCAAAACCGAAAAATAGATATGAGACTTGCAGCATATATCATAGGT
ATTAAACGTACAGCAGAAGCAGCTAGATATCGTGGTTGGGCATAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1080
1140
1200
1245
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0861 [new locus tag: SAUSA300_RS04645 ]
- symbol: GudB
- description: NAD-specific glutamate dehydrogenase
- length: 414
- theoretical pI: 4.92895
- theoretical MW: 45760.7
- GRAVY: -0.21087
⊟Function[edit | edit source]
- ⊞reaction: EC 1.4.1.2? ExPASy
- TIGRFAM: Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 11.9)
- ⊞TheSEED :
- NAD-specific glutamate dehydrogenase (EC 1.4.1.2)
Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamate dehydrogenases NAD-specific glutamate dehydrogenase (EC 1.4.1.2)and 1 more - ⊞PFAM: NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 262.5)and 3 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEYSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGIKRTAEAARYRGWA
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- ⊟interaction partners:
SAUSA300_2187 (rpsE) 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_1139 (sucD) succinyl-CoA synthetase subunit alpha [1] (data from MRSA252) SAUSA300_1852 putative ABC transporter ATP-binding protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jump up to: 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)