From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0828 [new locus tag: NWMN_RS04675 ]
  • pan locus tag?: SAUPAN003060000
  • symbol: gudB
  • pan gene symbol?: gudB
  • synonym:
  • product: NAD-specific glutamate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0828 [new locus tag: NWMN_RS04675 ]
  • symbol: gudB
  • product: NAD-specific glutamate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 915759..917045
  • length: 1287
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    TTGAAAGCGAAATCATTAGGTAAAGAAGAGGCGAAAAGGATCATGACTGAGAACAATAAT
    TTAGTAACTTCTACTCAAGGAATTATTAAAGAAGCATTGCATAAATTGGGATTTGACGAA
    GGAATGTACGATTTAATTAAAGAACCTTTAAGAATGTTACAAGTGCGTATCCCTGTACGA
    ATGGATGATGGCACAGTAAAAACATTCACAGGTTACCGTGCGCAACATAATGATGCTGTT
    GGACCAACAAAAGGGGGCGTGCGTTTCCACCCAGATGTTGATGAAGAAGAAGTAAAAGCA
    TTATCAATGTGGATGACTTTGAAATGTGGCATTGTAAACTTACCATACGGTGGTGGTAAG
    GGTGGTATCGTTTGTGATCCACGTCAAATGAGCATTCATGAAGTTGAACGTTTATCACGC
    GGATATGTAAGAGCAATTTCACAATTCGTAGGTCCGAACAAAGATATTCCAGCACCAGAT
    GTATTTACAAACTCACAAATTATGGCTTGGATGATGGATGAATATAGTGCATTAGATAAA
    TTTAATTCACCAGGTTTCATCACAGGTAAACCAATTGTATTGGGTGGTTCTCATGGACGC
    GACAGATCAACTGCACTAGGTGTAGTTATTGCAATTGAACAAGCTGCAAAACGTCGTAAT
    ATGCAAATTGAAGGTGCCAAGGTTGTTATTCAAGGTTTCGGTAATGCCGGAAGTTTCTTA
    GCTAAATTCTTATATGATTTAGGTGCAAAAATTGTAGGTATCTCTGATGCTTACGGTGCA
    TTACACGATCCAAATGGCTTAGATATAGATTATTTATTAGACCGTCGTGATAGTTTTGGT
    ACGGTAACAAATTTATTTGAAGAAACAATCTCAAATAAAGAATTGTTTGAATTAGATTGT
    GACATTTTAGTACCAGCGGCTATTTCAAACCAAATTACAGAAGACAATGCACATGATATT
    AAAGCTAGTATCGTTGTTGAAGCTGCTAATGGACCTACAACACCAGAAGCAACACGTATT
    TTAACTGAACGTGGTATATTATTAGTTCCAGACGTATTAGCAAGTGCTGGTGGTGTAACG
    GTTTCTTACTTCGAATGGGTACAAAATAATCAAGGTTATTATTGGTCTGAAGAAGAAGTT
    AATGAAAAGCTACGTGAAAAATTAGAAGCGGCATTTGATACGATTTACGAATTGTCTCAA
    AACCGAAAAATAGATATGAGACTTGCAGCATATATCATAGGTATTAAACGTACAGCAGAA
    GCAGCTAGATATCGTGGTTGGGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0828 [new locus tag: NWMN_RS04675 ]
  • symbol: GudB
  • description: NAD-specific glutamate dehydrogenase
  • length: 428
  • theoretical pI: 5.36927
  • theoretical MW: 47330.6
  • GRAVY: -0.23785

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 11.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 262.1)
    and 3 more
    AA_dh_N (CL0603) ELFV_dehydrog_N; Glu/Leu/Phe/Val dehydrogenase, dimerisation domain (PF02812; HMM-score: 188.6)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.7)
    no clan defined CobS_N; Cobaltochelatase CobS subunit N terminal (PF12556; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003234
    • TAT(Tat/SPI): 0.000353
    • LIPO(Sec/SPII): 0.000477
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKAKSLGKEEAKRIMTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEYSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGIKRTAEAARYRGWA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_1156(sucD)succinyl-CoA synthetase subunit alpha  [1] (data from MRSA252)
    NWMN_1763ABC transporter ATP-binding protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_0828&oldid=58735"