⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_0308 [new locus tag: NWMN_RS01755 ]
pan locus tag^?: SAUPAN000622000
symbol: NWMN_0308
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0308 [new locus tag: NWMN_RS01755 ]
• symbol: NWMN_0308
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 357446..357745
• length: 300
• essential: unknown

⊟Accession numbers

• Gene ID: 5330147 NCBI
• RefSeq: YP_001331342 NCBI
• BioCyc:
• MicrobesOnline: 3705839 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  
  ATGTTTGGATTTACCAAACGACATGAACAAGATTGGCGTTTAACGCGATTAGAAGAAAAT
  GATAAGACTATGTTTGAAAAATTCGACAGAATAGAAGATAGTCTTAGAGCGCAAGAAAAG
  ATTTATGACAAATTAGATAGAAATTTTGAAGAATTAAAGCGCGACAAGGTAGAAGATGAA
  AAGAATAAAGAAAAGAATGCCAAGAATATTAGAGACATAAAAATGTGGATTCTAGGTTTG
  ATAGGGACTATCTTCAGTACGATTGTCATAGCTTTACTAAGAACTGTTTTTGGTATTTAA
  60
  120
  180
  240
  300
  

⊟Protein

⊟General

• locus tag: NWMN_0308 [new locus tag: NWMN_RS01755 ]
• symbol: NWMN_0308
• description: hypothetical protein
• length: 99
• theoretical pI: 9.02274
• theoretical MW: 12010.8
• GRAVY: -0.737374

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism Other MutS2 family protein (TIGR01069; HMM-score: 8.5)
• TheSEED  :
  • Phage phi 11 orf48 protein homolog
• ⊞⊞PFAM:
  no clan defined DUF2951; Protein of unknown function (DUF2951) (PF11166; HMM-score: 178.4)
  and 10 more
  XhlA; Haemolysin XhlA (PF10779; HMM-score: 19.9)
  MpPF26; M penetrans paralogue family 26 (PF07666; HMM-score: 17.4)
  TUNAR; TUNAR (PF21954; HMM-score: 15.6)
  TPR (CL0020) Sec3_C_2; Sec3 exocyst complex subunit (PF15278; HMM-score: 14.7)
  no clan defined TelA; Toxic anion resistance protein (TelA) (PF05816; HMM-score: 14.3)
  DUF2207_C; Predicted membrane protein (DUF2207) C-terminal domain (PF20990; HMM-score: 13.6)
  YxiF; YxiF protein (PF24715; HMM-score: 13.6)
  Cyclin (CL0065) DUF3452; Domain of unknown function (DUF3452) (PF11934; HMM-score: 13.4)
  no clan defined DRAT; Dinitrogenase reductase ADP-ribosyltransferase (DRAT) (PF07357; HMM-score: 11.5)
  PBDC1; PBDC1 protein (PF04669; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.78
  • Cytoplasmic Membrane Score: 8.16
  • Cellwall Score: 0.06
  • Extracellular Score: 0.01
  • Internal Helix: 1
• ⊞⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0212
  • Cytoplasmic Membrane Score: 0.8518
  • Cell wall & surface Score: 0.0017
  • Extracellular Score: 0.1254
• ⊞⊞LocateP: Intracellular /TMH start AFTER 60
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: Possibly Sec-
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002331
  • TAT(Tat/SPI): 0.000943
  • LIPO(Sec/SPII): 0.00094
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151220520 NCBI
• RefSeq: YP_001331342 NCBI
• UniProt: A0A0H3KAN8 UniProt

⊟Protein sequence

• MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRAQEKIYDKLDRNFEELKRDKVEDEKNKEKNAKNIRDIKMWILGLIGTIFSTIVIALLRTVFGI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications