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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1881 [new locus tag: SAUSA300_RS10295 ]
- pan locus tag?: SAUPAN004927000
- symbol: gatA
- pan gene symbol?: gatA
- synonym:
- product: aspartyl/glutamyl-tRNA amidotransferase subunit A
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1881 [new locus tag: SAUSA300_RS10295 ]
- symbol: gatA
- product: aspartyl/glutamyl-tRNA amidotransferase subunit A
- replicon: chromosome
- strand: -
- coordinates: 2042488..2043945
- length: 1458
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914028 NCBI
- RefSeq: YP_494533 NCBI
- BioCyc: see SAUSA300_RS10295
- MicrobesOnline: 1293396 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGAGCATTCGCTACGAATCGGTTGAGAATTTATTAACTTTAATAAAAGACAAAAAAATC
AAACCATCTGATGTTGTTAAAGATATATATGATGCAATTGAAGAGACTGATCCAACAATT
AAGTCTTTTCTAGCGCTGGATAAAGAAAATGCAATCAAAAAAGCGCAAGAATTGGATGAA
TTACAAGCAAAAGATCAAATGGATGGCAAATTATTTGGTATTCCAATGGGTATAAAAGAT
AACATTATTACAAACGGATTAGAAACAACATGTGCAAGTAAAATGTTAGAAGGTTTTGTG
CCAATTTACGAATCTACTGTAATGGAAAAACTACATAATGAAAATGCCGTTTTAATCGGT
AAATTAAATATGGATGAGTTTGCAATGGGTGGTTCAACAGAAACATCTTATTTCAAAAAA
ACAGTTAACCCATTTGACCATAAAGCAGTGCCAGGTGGTTCATCAGGTGGATCTGCAGCA
GCAGTTGCAGCTGGCTTAGTACCATTTAGCTTAGGTTCAGACACAGGTGGTTCAATTAGA
CAACCGGCTGCATATTGTGGCGTTGTCGGTATGAAACCAACATACGGTCGTGTATCTCGA
TTTGGATTAGTTGCTTTTGCATCTTCATTAGACCAAATTGGTCCATTGACTCGAAATGTA
AAAGATAATGCAATCGTATTAGAAGCTATTTCTGGTGCAGATGTTAATGACTCTACAAGT
GCACCAGTTGATGATGTAGACTTTACATCTGAAATTGGTAAAGATATTAAAGGATTAAAA
GTTGCATTACCTAAAGAATACTTAGGTGAAGGTGTAGCTGATGACGTAAAAGAAGCAGTT
CAAAACGCTGTAGAAACTTTAAAATCTTTAGGTGCTGTCGTTGAGGAAGTATCATTGCCA
AATACTAAATTTGGTATTCCATCATATTACGTGATTGCATCATCAGAAGCTTCGTCAAAC
CTTTCTCGTTTTGACGGAATTCGTTATGGTTATCATTCTAAAGAAGCTCATTCATTAGAA
GAATTATATAAAATGTCAAGATCTGAAGGTTTCGGTAAAGAAGTAAAACGTCGTATTTTC
TTAGGTACATTTGCATTAAGTTCAGGTTACTATGATGCTTACTATAAAAAATCTCAAAAA
GTTAGAACATTGATTAAAAATGACTTTGATAAAGTATTCGAAAATTATGATGTAGTAGTT
GGTCCAACAGCGCCTACAACTGCGTTTAATTTAGGTGAAGAAATTGATGATCCATTAACA
ATGTATGCCAATGATTTATTAACAACACCAGTAAACTTAGCTGGATTACCTGGTATTTCT
GTTCCTTGTGGACAATCAAATGGCCGACCAATCGGTTTACAGTTCATTGGTAAACCATTC
GATGAAAAAACGTTATATCGTGTCGCTTATCAATATGAAACACAATACAATTTACATGAC
GTTTATGAAAAATTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1881 [new locus tag: SAUSA300_RS10295 ]
- symbol: GatA
- description: aspartyl/glutamyl-tRNA amidotransferase subunit A
- length: 485
- theoretical pI: 4.76401
- theoretical MW: 52820.5
- GRAVY: -0.234639
⊟Function[edit | edit source]
- ⊞reaction: EC 6.3.5.7? ExPASy
- ⊞TIGRFAM: Protein synthesis tRNA aminoacylation aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (TIGR00132; EC 6.3.5.-; HMM-score: 657.8)and 2 more
- TheSEED :
- Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6)
- Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)
- ⊞PFAM: no clan defined Amidase; Amidase (PF01425; HMM-score: 526.9)and 1 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- ⊟interaction partners:
SAUSA300_0533 (tuf) elongation factor Tu [1] (data from MRSA252) SAUSA300_1656 universal stress protein [1] (data from MRSA252) SAUSA300_2484 hydroxymethylglutaryl-CoA synthase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: gatB < gatA < gatC
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jump up to: 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)