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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2484 [new locus tag: SAUSA300_RS13795 ]
  • pan locus tag?: SAUPAN006207000
  • symbol: SAUSA300_2484
  • pan gene symbol?: mvaS
  • synonym:
  • product: hydroxymethylglutaryl-CoA synthase

A crystal structure is available at: 1TVZ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2484 [new locus tag: SAUSA300_RS13795 ]
  • symbol: SAUSA300_2484
  • product: hydroxymethylglutaryl-CoA synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2682929..2684095
  • length: 1167
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACAATAGGTATCGATAAAATAAACTTTTACGTTCCAAAGTACTATGTAGACATGGCT
    AAATTAGCAGAAGCACGCCAAGTAGACCCAAACAAATTTTTAATTGGAATTGGTCAAACT
    GAAATGGCTGTTAGTCCTGTAAACCAAGACATCGTTTCAATGGGCGCTAACGCTGCTAAG
    GACATTATAACAGACGAAGACAAAAAGAAAATTGGTATGGTAATTGTGGCAACTGAATCA
    GCAGTTGATGCTGCTAAAGCAGCCGCTGTTCAAATTCACAACTTATTAGGTATTCAACCT
    TTTGCACGCTGCTTTGAAATGAAAGAAGCTTGTTATGCTGCAACACCAGCAATTCAATTA
    GCTAAAGATTATTTAGCAACTAGACCGAATGAAAAAGTATTAGTTATTGCTACAGATACA
    GCACGTTATGGATTGAATTCAGGCGGCGAGCCAACACAAGGTGCTGGCGCAGTTGCGATG
    GTTATTGCACATAATCCAAGCATTTTGGCATTAAATGAAGATGCTGTTGCTTACACTGAA
    GACGTTTATGATTTCTGGCGTCCAACTGGACATAAATATCCATTAGTTGATGGTGCATTA
    TCTAAAGATGCTTATATCCGCTCATTCCAACAAAGCTGGAATGAATACGCAAAACGTCAA
    GGTAAGTCGCTAGCTGACTTCGCATCTCTATGCTTCCATGTTCCATTTACAAAAATGGGT
    AAAAAGGCATTAGAGTCAATCATTGATAACGCTGATGAAACAACTCAAGAGCGTTTACGT
    TCAGGATATGAAGATGCTGTAGATTATAACCGTTATGTCGGTAATATTTATACTGGATCA
    TTATATTTAAGCCTAATATCATTACTTGAAAATCGAGATTTACAAGCTGGTGAAACAATC
    GGTTTATTCAGTTATGGCTCAGGTTCAGTTGGTGAATTTTATAGTGCGACATTAGTTGAA
    GGCTACAAAGATCATTTAGATCAAGCTGCACATAAAGCATTATTAAATAACCGTACTGAA
    GTATCTGTTGATGCATATGAAACATTCTTCAAACGTTTTGATGACGTTGAATTTGACGAA
    GAACAAGATGCTGTTCATGAAGATCGTCATATTTTCTACTTATCAAATATTGAAAATAAC
    GTTCGTGAATATCACAGACCAGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2484 [new locus tag: SAUSA300_RS13795 ]
  • symbol: SAUSA300_2484
  • description: hydroxymethylglutaryl-CoA synthase
  • length: 388
  • theoretical pI: 4.67485
  • theoretical MW: 43205.2
  • GRAVY: -0.295361

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.3.10?  ExPASy
    Hydroxymethylglutaryl-CoA synthase Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
  • TIGRFAM:
    hydroxymethylglutaryl-CoA synthase (TIGR01835; EC 2.3.3.10; HMM-score: 531.4)
    and 3 more
    hydroxymethylglutaryl-CoA synthase (TIGR01833; EC 2.3.3.10; HMM-score: 160.5)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative hydroxymethylglutaryl-CoA synthase (TIGR00748; EC 2.3.3.10; HMM-score: 98.4)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 43.1)
  • TheSEED  :
    • Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10)
    and 1 more
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10)
  • PFAM:
    Thiolase (CL0046) HMG_CoA_synt_C; Hydroxymethylglutaryl-coenzyme A synthase C terminal (PF08540; HMM-score: 96.8)
    and 4 more
    HMG_CoA_synt_N; Hydroxymethylglutaryl-coenzyme A synthase N terminal (PF01154; HMM-score: 69.1)
    Chal_sti_synt_N; Chalcone and stilbene synthases, N-terminal domain (PF00195; HMM-score: 27)
    ACP_syn_III; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (PF08545; HMM-score: 26.1)
    ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 17.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006327
    • TAT(Tat/SPI): 0.000235
    • LIPO(Sec/SPII): 0.000473
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAVSPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFARCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAMVIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAKRQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNIYTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKALLNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Carl J Balibar, Xiaoyu Shen, Jianshi Tao
    The mevalonate pathway of Staphylococcus aureus.
    J Bacteriol: 2009, 191(3);851-61
    [PubMed:19028897] [WorldCat.org] [DOI] (I p)    Nino Campobasso, Mehul Patel, Imogen E Wilding, Howard Kallender, Martin Rosenberg, Michael N Gwynn
    Staphylococcus aureus 3-hydroxy-3-methylglutaryl-CoA synthase: crystal structure and mechanism.
    J Biol Chem: 2004, 279(43);44883-8
    [PubMed:15292254] [WorldCat.org] [DOI] (P p)
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