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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2477 [new locus tag: SAUSA300_RS13745 ]
  • pan locus tag?: SAUPAN006196000
  • symbol: cidC
  • pan gene symbol?: cidC
  • synonym:
  • product: pyruvate oxidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2477 [new locus tag: SAUSA300_RS13745 ]
  • symbol: cidC
  • product: pyruvate oxidase
  • replicon: chromosome
  • strand: -
  • coordinates: 2674643..2676382
  • length: 1740
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAAAAATAAAAGCAAATGAAGCATTAGTTAAAGCATTACAAGCATGGGATATAGAT
    CACTTGTATGGTATTCCAGGAGACTCAATCGACGCAGTAGTCGATAGTTTACGTACAGTG
    AGAGATCAATTTAAATTTTATCATGTACGTCATGAAGAAGTAGCAAGCTTAGCGGCTGCT
    GGTTACACAAAATTAACTGGTAAAATCGGTGTGGCATTAAGTATCGGTGGCCCTGGTTTA
    ATTCATTTATTAAATGGTATGTATGATGCCAAAATGGATAATGTACCGCAATTAATATTA
    TCTGGACAAACGAATAGTACAGCACTTGGAACGAAAGCATTCCAAGAAACAAATTTACAA
    AAATTATGTGAAGATGTAGCCGTTTATAATCACCAAATTGAAAAAGGTGACAATGTGTTT
    GAAATCGTTAACGAAGCAATTCGTACGGCATATGAACAAAAAGGTGTAGCTGTTGTTATT
    TGTCCTAACGACTTATTAACTGAAAAAATTAAAGATACAACGAATAAACCAGTAGATACA
    TCAAGACCAACAGTAGTATCACCAAAATATAAAGACATCAAAAAAGCGGTTAAACTAATT
    AATAAAAGTAAAAAGCCTGTCATGTTAATTGGTGTAGGTGCGAAACATGCGAAAGATGAG
    CTACGTGAATTTATTGAAATGGCTAAAATTCCTGTCATTCATTCATTACCAGCTAAAACA
    ATCTTGCCGGATGATCATCCATATAGTATCGGTAACTTAGGTAAAATCGGTACCAAAACA
    TCTTATCAAACAATGCAGGAAGCGGATTTATTAATTATGGTTGGTACAAACTATCCATAT
    GTGGATTACTTACCTAAGAAAAATATTAAAGCCATTCAAATTGACACAAATCCTAAAAAT
    ATCGGACATCGTTTCAATATTAATGTAGGAATTGTTGGAGATAGTAAAATTGCGTTGCAT
    CAGTTAACTGAAAATATTAAACATGTTGCTGAAAGACCATTCTTAAACAAAACGTTAGAA
    CGTAAAGCGGTTTGGGATAAATGGATGGAACAAGATAAAAATAATAATAGTAAACCATTA
    CGTCCAGAACGATTAATGGCATCAATCAATAAATTTATTAAAGATGATGCAGTGATTTCA
    GCAGATGTAGGTACAGCAACAGTTTGGTCAACTCGATACTTAAACCTTGGTGTAAATAAC
    AAGTTCATCATTTCAAGTTGGTTAGGTACAATGGGTTGCGGTCTTCCAGGTGCAATTGCA
    TCAAAAATTGCATATCCAAATAGACAAGCCATCGCAATTGCTGGTGACGGTGCATTCCAA
    ATGGTAATGCAAGACTTCGCTACAGCAGTACAATATGATTTACCTTTAACTGTATTTGTA
    CTTAATAACAAACAGTTAGCATTTATTAAATATGAACAACAAGCAGCTGGTGAATTAGAA
    TATGCAGTTGATTTTTCTGATATGGATCATGCAAAATTTGCTGAGGCAGCAGGTGGTAAA
    GGTTATACAATTAAGAGTGCTAGCGAAGTAGATGCTATAGTCGAAGAGGCATTAGCACAA
    GATGTACCAACGATTGTAGATGTATATGTTGATCCTAATGCTGCGCCATTACCAGGTAAA
    ATTGTAAATGAAGAAGCGCTTGGTTATGGTAAGTGGGCATTTAGATCAATTACTGAAGAT
    AAACATTTAGATTTAGATCAAATTCCACCAATTTCAGTGGCAGCAAAACGTTTCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2477 [new locus tag: SAUSA300_RS13745 ]
  • symbol: CidC
  • description: pyruvate oxidase
  • length: 579
  • theoretical pI: 7.43776
  • theoretical MW: 63756.9
  • GRAVY: -0.172193

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.3.3?  ExPASy
    Pyruvate oxidase Pyruvate + phosphate + O2 = acetyl phosphate + CO2 + H2O2
  • TIGRFAM:
    Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 492.7)
    and 14 more
    Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 367.2)
    Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 270.3)
    Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 259)
    glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 187.7)
    Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 184.3)
    Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 174.2)
    indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 94.9)
    Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 93.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 29)
    phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 27.5)
    sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 20)
    Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 15)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 14.6)
    Metabolism Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 14.6)
  • TheSEED  :
    • Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)
    • Pyruvate oxidase, CidC
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)
    and 1 more
    Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  Pyruvate oxidase, CidC
  • PFAM:
    THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 138.5)
    TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 134.4)
    FAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 117)
    and 3 more
    THDP-binding (CL0254) POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 15.8)
    FAD_DHS (CL0085) CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 13.7)
    no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004655
    • TAT(Tat/SPI): 0.00018
    • LIPO(Sec/SPII): 0.000379
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVICPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLDQIPPISVAAKRFL

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

This enzyme is induced during biofilm development as a programmed cell death pathway. The exact process is poorly understood but is thought to require intracellular acidification by the CidC-mediated conversion of pyruvate into acetate and carbon dioxide, the generation of reactive oxygen species and, potentially, breakdown of cell envelope integrity by CidB.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Toni G Patton, Kelly C Rice, Mary K Foster, Kenneth W Bayles
    The Staphylococcus aureus cidC gene encodes a pyruvate oxidase that affects acetate metabolism and cell death in stationary phase.
    Mol Microbiol: 2005, 56(6);1664-74
    [PubMed:15916614] [WorldCat.org] [DOI] (P p)    Vinai Chittezham Thomas, Marat R Sadykov, Sujata S Chaudhari, Joselyn Jones, Jennifer L Endres, Todd J Widhelm, Jong-Sam Ahn, Randeep S Jawa, Matthew C Zimmerman, Kenneth W Bayles
    A central role for carbon-overflow pathways in the modulation of bacterial cell death.
    PLoS Pathog: 2014, 10(6);e1004205
    [PubMed:24945831] [WorldCat.org] [DOI] (I e)    Kirsi Savijoki, Malena Skogman, Adyary Fallarero, Tuula A Nyman, Antti Sukura, Pia Vuorela, Pekka Varmanen
    Penicillin G increases the synthesis of a suicidal marker (CidC) and virulence (HlgBC) proteins in Staphylococcus aureus biofilm cells.
    Int J Med Microbiol: 2016, 306(1);69-74
    [PubMed:26725755] [WorldCat.org] [DOI] (I p)
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