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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0228 [new locus tag: NWMN_RS01270 ]
  • pan locus tag?: SAUPAN001190000
  • symbol: NWMN_0228
  • pan gene symbol?: esaD
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0228 [new locus tag: NWMN_RS01270 ]
  • symbol: NWMN_0228
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 287539..289392
  • length: 1854
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    GTGCATGACATGACAAAAGATATTGAATATCTAACAGCTGATTATGACAATGAAAAGTCA
    TCTATCCAAAGTGTAATAGATGCAATAGAGGGTCAAGACTTCTTAGATGTAGATACAACA
    ATGGATGATGCGGTAAGCGATGTCAGTTCTTTAGACGAAGATGGCGCAATATCATTAACA
    AGTAGTGTAGTAGGTCCACAAGGATCTAAATTAATGGGGTATTATCAAAATGAGTTATAT
    GATTATGCATCTCAATTAGATTCGAAAATGAAAGAAATTATTGACACGCCATTTATAGAA
    GATATAGATAAAGCATTCAAAGGTATAACGAATGTTAAATTGGAAAATATACTAATTAAA
    AATGGTGGTGGACATGGTAGAGATACCTATGGGGCTTCTGGGAAAATTGCAAAGGGAGAT
    GCCAAGAAAAGTGACAGCGATGTTTATAGCATCGATGAAATATTAAAATCGGATCAAGAA
    TTTGTAAAAGTAATTGATCAGCATTACAAAGAAATGAAAAAAGAAGATAAGAAATTATCT
    AAAAGTGATTTTGAAAAAATGATGACTCAGGGCGCTTCTTGTGATTACATGACAGTAGCT
    GAAGCGGAAGAGCTAGAGGAGCAAAAGAAAAAAGAAGAAGCTATAGAGATTGCAGCACTA
    GCTGGTATGGTAGTTTTATCTTGTATTAATCCTGTTGCTGGAGCAGTAGCTATTGGTGCT
    TATTCCGCTTATTCAGCAGCAAATGCAGCCACAGGAAAAAATATTGTAACTGGAAGAAAG
    CTATCTAAAGAAGAACGAATCATGGAAGGACTTTCGCTTATTCCATTGCCAGGTATGGGC
    TTCCTCAAAGGTGCTGGGAAAAGTTTAATGAAATTAGGCTTCAAAGGCGGAGAAAAATTT
    GCAGTTAAAACAGGATTGCAAAAGACAATGCAACAAGCAGTTAGTCGTATTTCACCTAAA
    ATGGGAATGATGAAAAACAGTGTGTTGAATCAATCTCGTAACTTTGCTCAAAATACTCAT
    GTTGGACAAATGCTGAGTAACATGCGTGGTCAAGCAACTCATACTGTTCAACAAAGTAGA
    AATTGGATTGGACAACAAGCACAAAATGTCAAACGAATAGTGAATAATGGACTTGATAAA
    GAAATAGCACATCCATTTAAACAACAACTTGCACCAGCGGGAATGGGTGGTATAAAATTT
    GCTGAAACAACTACTTTGAGAAACATGGGTCAAAACATAAAGCGTGCTGTTACACCACAA
    AATCACGTGACACATGGTCCAAAAGATAGTATGGTGAGAAGTGAAGGCAAACATAGTATA
    AGTAGCCATGAAATGAATTCATCAAAGTATGTTGAATCACCAAACTACACCAAGGTTGAA
    TTCGGAGAACACTATGCAAGACTTAGACCTAAGAAACTAAAGGCGAATATTGAATACACA
    ACACCTACTGGTCACATATATCGAACCGATCATAAAGGTCGCATAAAAGAAGTTTATGTA
    GACAATCTCTCTCTAAAAGATGGGGATCGTAATAGCCATGCACAAAGAACTGTGGGGGGA
    GAGGATAGATTACCAGACGATGATGGAGGTCACTTAATCGCTAGAATGTTTGGTGGTTCA
    AAAGACATTGATAACCTTGTGGCACAAAGTAAATTTATCAACCGTCCATTTAAGGAGAAA
    GGTCATTGGTATAATCTTGAGAAAGAGTGGCAAGAGTTCTTAAACTCTGGGAAAGAGGTG
    AAAAATATTAAAATGGAAGTAAAATATAGCGGTAATAGTCAAAGACCGACTATATTTAAA
    GTTGAATATGAAATTAATGGTGAAAGAAATATTAGAAGAATATTAAATAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1854

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0228 [new locus tag: NWMN_RS01270 ]
  • symbol: NWMN_0228
  • description: hypothetical protein
  • length: 617
  • theoretical pI: 8.70293
  • theoretical MW: 68701.3
  • GRAVY: -0.661426

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Membrane TransportProtein secretion system, Type VIIESAT-6 proteins secretion system in Firmicutes Lmo0066 homolog within ESAT-6 gene cluster, similarity to ADP-ribosylating toxins 
  • PFAM:
    His-Me_finger (CL0263) Endonuclea_NS_2; DNA/RNA non-specific endonuclease (PF13930; HMM-score: 108.8)
    no clan definedPT-TG; Pre-toxin TG (PF14449; HMM-score: 34.2)
    FAM180; FAM180 family (PF15173; HMM-score: 13.1)
    EpsM (CL0331) Phage_lysis; Bacteriophage Rz lysis protein (PF03245; HMM-score: 13.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 70
    • Cmax: 0.143
    • Cmax_pos: 29
    • Smax: 0.116
    • Smax_pos: 70
    • Smean: 0.065
    • D: 0.086
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHDMTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSDVSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKAFKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVKVIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALAGMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGMGFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQNTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGMGGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVESPNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRNSHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKEWQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Mark Anderson, Yi-Hsing Chen, Emily K Butler, Dominique M Missiakas
EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus.
J. Bacteriol.: 2011, 193(7);1583-9
[PubMed:21278286] [WorldCat.org] [DOI] (I p)
Zhenping Cao, M Guillermina Casabona, Holger Kneuper, James D Chalmers, Tracy Palmer
The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.
Nat Microbiol: 2016, 2;16183
[PubMed:27723728] [WorldCat.org] [DOI] (I e)