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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1699 [new locus tag: NWMN_RS09545 ]
  • pan locus tag?: SAUPAN004550000
  • symbol: hsdS
  • pan gene symbol?: hsdS2
  • synonym:
  • product: type I restriction modification system, site specificity determination subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1699 [new locus tag: NWMN_RS09545 ]
  • symbol: hsdS
  • product: type I restriction modification system, site specificity determination subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1896174..1897373
  • length: 1200
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGTAATACACAAAAGAAAAATGTGCCAGAATTGAGATTCCCAGGATTTGAAGGCGAA
    TGGGAAGAGAAGAAGTTAGGGAATCTTACTACCAAAATAGGTAGTGGAAAGACTCCCAAA
    GGTGGAAGTGAAAACTATACAAACAAAGGCATACCATTTTTAAGGAGTCAAAATATTAGA
    AATGGTAAATTAAATCTTAATGACTTAGTTTATATTAGTAAAGATATAGATGATGAGATG
    AAAAATAGTAGAACGTACTATGGTGATGTTCTTTTAAATATTACAGGAGCATCAATAGGT
    AGAACAGCCATTAATTCGATAGTTGAAACGCATGCTAATTTAAATCAACATGTATGTATT
    ATTAGATTGAAAAAAGAGTATTATTATAATTTTTTTGGACAGTATCTATTATCAAGAAAA
    GGTAAAAGGAAAATTTTCCTTGCACAAAGTGGAGGTAGTCGAGAAGGTCTAAACTTCAAA
    GAAATTGCTAATTTAAAAATCTTCACCCCAACTATATTTGAAGAACAGCAAAAAATAGGC
    AAGTTCTTCAGCAAACTCGACCGACAAATTGAATTAGAAGAACAAAAACTTGAATTGCTT
    CAACAACAGAAAAAAGGCTATATGCAGAAAATCTTCACACAAGAATTGCGATTCAAAGAT
    GAGAATGGTGAAGAATATCCAGAGTGGGAAAACAAATTCATAAAAGACATCTTTATCTTT
    GAAAATAATAGAAGAAAACCAATTACTTCTTCATTAAGAGAAAAGGGGTTATACCCTTAC
    TATGGTGCAACTGGAATTATTGATTACGTAAAAGATTATTTATTCAATAATGAAGAACGA
    TTACTAATAGGAGAAGATGGTGCAAAATGGGGGCAGTTTGAGACGAGTAGCTTTATTGCT
    AATGGGCAATACTGGGTAAATAATCATGCGCATGTAGTTAAAAGTAATGATCATAATTTG
    TTTTTTATGAATTATTATTTAAATTTTAAAGAACTACGAGCATTTGTCACAGGTAATGCA
    CCAGCTAAATTAACTCATGCGAACTTATGCAATATAAATCTTAAAATACCTTGTCTCACT
    GAACAAGATAAAGTAAGTGCATTGTTAAAATCTATAGACAATAAAATGAATAATCAAATG
    AATAGAATTGAGTTATTAAAAGAACGTAAAAAAGGACTATTACAAAAAATGTTTATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1699 [new locus tag: NWMN_RS09545 ]
  • symbol: HsdS
  • description: type I restriction modification system, site specificity determination subunit
  • length: 399
  • theoretical pI: 9.81387
  • theoretical MW: 46467.8
  • GRAVY: -0.66015

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesCell divisionseptum site-determining protein MinD (TIGR01968; HMM-score: 13.4)
  • TheSEED:  
    DNA MetabolismDNA Metabolism - no subcategoryRestriction-Modification System Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) 
    DNA Metabolism - no subcategoryType I Restriction-Modification Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) 
  • PFAM:
    TRD (CL0477) Methylase_S; Type I restriction modification DNA specificity domain (PF01420; HMM-score: 143.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.142
    • Ymax_pos: 11
    • Cmax: 0.115
    • Cmax_pos: 43
    • Smax: 0.29
    • Smax_pos: 2
    • Smean: 0.183
    • D: 0.158
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]