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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2066 [new locus tag: NWMN_RS11925 ]
  • pan locus tag?: SAUPAN005511000
  • symbol: tnp
  • pan gene symbol?: tnp4
  • synonym:
  • product: transposase for IS1181

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2066 [new locus tag: NWMN_RS11925 ]
  • symbol: tnp
  • product: transposase for IS1181
  • replicon: chromosome
  • strand: +
  • coordinates: 2294897..2296135
  • length: 1239
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    GTGACCAAACCCAAACTAAAGGAGACAAGTGCGCCTATGTGTAATGATACCTTAGAATTA
    CTAAGAATAAAAGATGAAAATATAAAATATATAAACCAAGAAATTGACGTCATTATCAAA
    GGAAAAAAAGCAACAGTGGTTAATGCTGTACTAACGTATAAGCCTTCGGCCTGTTATTGT
    TGTGGAGTTAAAAATGAAGGACAAATTCATAAACATGGTAAGCGTGTTTCTCGTATTACT
    TTACTTAAAACTCAAGGGTATAACACATACCTCAACTTAGCTAAACAACGTTTTAAATGC
    TTAGAATGCAATGGCACTTTTACTGCTAAAACGTCAATTGTTGATGAGTCGTGTTTTATC
    TCAAGATGTGTTACTCAAAAAGTTATAGAAGAAGCTACTAAAGTTAAAACAGAGATTGAT
    ACTGCAGAAGATAACTGTATCTCTCCATCTACTGTAAGTCGTATTAGAACTAAAGCGGCT
    AATTCATTACGAATTAAACCCTTTAATTGTTTGCCAGAACACATCGCTATGGATGAATTT
    AAAAGCGTTAAAAATGTAACTGGATCAATGAGTTTCATTTTTATAGATAATGATACTCAT
    GATGTTATAGATATTTTAGAAAATAGAACTACAAGATTCTTGCGTGCCTATTTCGAGCGA
    TTCGATTTAAAAAATCGACAACAAGTTAAGACGGTTACTATTGACATGTATGAACCCTAT
    GTCCGATTATTTCGCGACCTATTTCCTAATGCAGCTATTATTTTTGACAGATTCCATATC
    GTTCAACATTTAAATAGAGAACTTAATAAGTATCGTGTACAAGTTATGAATGAATACCGT
    AATAAAAAAGGACCTGATTATACAATTTTTAAGAATAACTGGAAAGTCCTATTGATGGAT
    ACTAGTAAAACCATATTTAGTAAATACAGATGGAATAAATCTTTTAAGGCTTATAAACGC
    TCATCTGACATTGTAGAATTCATGCTTTCAAAAGACGATATACTACGACACTCCTACGAA
    CTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGT
    TTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTAT
    AGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCT
    ATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2066 [new locus tag: NWMN_RS11925 ]
  • symbol: Tnp
  • description: transposase for IS1181
  • length: 412
  • theoretical pI: 10.2563
  • theoretical MW: 48326.9
  • GRAVY: -0.486893

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesDetoxificationS-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.8)
    MetabolismCentral intermediary metabolismOne-carbon metabolismS-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.8)
  • TheSEED:  
    Mobile element protein 
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 144.9)
    Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 42.5)
    HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.7)
    no clan definedDUF3795; Protein of unknown function (DUF3795) (PF12675; HMM-score: 10.4)
    DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 11
    • Cmax: 0.104
    • Cmax_pos: 65
    • Smax: 0.331
    • Smax_pos: 1
    • Smean: 0.233
    • D: 0.188
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKPKLKETSAPMCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPSACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIVDESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLPEHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVKTVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYTIFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLRKDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • tnp no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]