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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09175 [old locus tag: NWMN_1633 ]
  • pan locus tag?: SAUPAN004407000
  • symbol: NWMN_RS09175
  • pan gene symbol?: murC
  • synonym:
  • product: UDP-N-acetylmuramate--L-alanine ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS09175 [old locus tag: NWMN_1633 ]
  • symbol: NWMN_RS09175
  • product: UDP-N-acetylmuramate--L-alanine ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1818622..1819935
  • length: 1314
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1818622..1819935) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1021
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    ATGACACACTATCATTTTGTCGGAATTAAAGGTTCTGGCATGAGTTCATTAGCACAAATC
    ATGCATGATTTAGGACATGAAGTTCAAGGATCGGATATTGAGAACTACGTATTTACAGAA
    GTTGCTCTTAGAAATAAGGGGATAAAAATATTACCATTTGATGCTAATAACATAAAAGAA
    GATATGGTAGTTATACAAGGTAATGCATTCGCGAGTAGCCATGAAGAAATAGTACGTGCA
    CATCAATTGAAATTAGATGTTGTAAGTTATAATGATTTTTTAGGACAGATTATTGATCAA
    TATACTTCAGTAGCTGTAACTGGTGCACATGGTAAAACTTCTACAACAGGTTTATTATCA
    CATGTTATGAATGGTGATAAAAAGACTTCATTTTTAATTGGTGATGGCACAGGTATGGGA
    TTGCCTGAAAGTGATTATTTCGCTTTTGAGGCATGTGAATATAGACGTCACTTTTTAAGT
    TATAAACCTGATTACGCAATTATGACAAATATTGATTTCGATCATCCTGATTATTTTAAA
    GATATTAATGATGTTTTTGATGCATTCCAAGAAATGGCACATAATGTTAAAAAAGGTATT
    ATTGCTTGGGGTGATGATGAACATCTACGTAAAATTGAAGCAGATGTTCCAATTTATTAT
    TATGGATTTAAAGATTCGGATGACATTTATGCTCAAAATATTCAAATTACGGATAAAGGT
    ACTGCTTTTGATGTGTATGTGGATGGTGAGTTTTATGATCACTTCCTGTCTCCACAATAT
    GGTGACCATACAGTTTTAAATGCATTAGCTGTAATTGCGATTAGTTATTTAGAGAAGCTA
    GATGTTACAAATATTAAAGAAGCATTAGAAACGTTTGGTGGTGTTAAACGTCGTTTCAAT
    GAAACTACAATTGCAAATCAAGTTATTGTAGATGATTATGCACACCATCCAAGAGAAATT
    AGTGCTACAATTGAAACAGCACGAAAGAAATATCCACATAAAGAAGTTGTTGCAGTATTT
    CAACCACACACTTTCTCTAGAACACAGGCATTTTTAAATGAATTTGCAGAAAGTTTAAGT
    AAAGCAGATCGTGTATTCTTATGTGAAATTTTTGGATCAATTAGAGAAAATACTGGCGCA
    TTAACGATACAAGATTTAATTGATAAAATTGAAGGTGCATCGTTAATTAATGAAGATTCT
    ATTAATGTATTAGAACAATTTGATAATGCTGTTATTTTATTTATGGGTGCAGGTGATATT
    CAAAAATTACAAAATGCATATTTAGATAAATTAGGCATGAAAAATGCGTTTTAA
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS09175 [old locus tag: NWMN_1633 ]
  • symbol: NWMN_RS09175
  • description: UDP-N-acetylmuramate--L-alanine ligase
  • length: 437
  • theoretical pI: 4.77031
  • theoretical MW: 49187.9
  • GRAVY: -0.222197

Function[edit source | edit]

  • reaction:
    EC 6.3.2.8?  ExPASy
    UDP-N-acetylmuramate--L-alanine ligaseATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 416.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 171.9)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 78.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 75.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 67.2)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 25.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidbifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 21.2)
  • TheSEED:
  • PFAM:
    no clan definedMur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 87.3)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 81.1)
    no clan definedMur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 73.2)
    SGNH_hydrolase (CL0264) DUF459; Protein of unknown function (DUF459) (PF04311; HMM-score: 11.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 11
    • Cmax: 0.129
    • Cmax_pos: 31
    • Smax: 0.368
    • Smax_pos: 2
    • Smean: 0.221
    • D: 0.182
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446072313 NCBI
  • RefSeq: WP_000150168 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALRNKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYTSVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLSYKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPIYYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISYLEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHKEVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEGASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS09175 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]