From AureoWiki
Jump to: navigation, search
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11090 [old locus tag: NWMN_1922 ]
  • pan locus tag?: SAUPAN005132000
  • symbol: NWMN_RS11090
  • pan gene symbol?:
  • synonym:
  • product: ATP-dependent helicase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS11090 [old locus tag: NWMN_1922 ]
  • symbol: NWMN_RS11090
  • product: ATP-dependent helicase
  • replicon: chromosome
  • strand: +
  • coordinates: 2128896..2129828
  • length: 933
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2128896..2129828) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGC
    CCTTGCGAAGTTGCTGTATCTTTAATTAAGGATTTATCAATAGTAAAAGTTTATTCATCT
    TATATTAATCCTCCTAATAATAGATATAACTTGAAAAACGCTAAAATACATAAAATACCT
    GAAGATGTCATATTAAAAGCACCTAAATATCCAGATATTTACCAAGAAATTCTCTATCTT
    TTAAAAGAATCACATTTAATTATTGCTCATAATGCACTTTTTGATATTTCAGTATTAAAA
    AATACTAATAATTATTATGACTTACCTGTTCCAAACTTCATGTATGTCGATAGTATAAAT
    ATCTTTAGAAGCTTCCACGCAATCTCTAGTTTTAAATTAGAAAATTTGTGTAGCTTATAT
    GATATCGATAAAGAAAAATTACATTCTGCTAAATTTGACGTGCTAGCTTTATCGAAGATG
    TTGATATCACTCGCTAAAAACAATCAGCATTATAGTGTATTAAAATTAATACATTATATG
    CCTAAGCAATACATTAGATTTAGCAAATATTCTAACTCTCCAACTAAACTTTTCGATTCA
    GGATTTCAAAAAATTCATATGAAAATATCTGAGATTAATAAAATAGAAGTGGAAAGTGTA
    ATCCCTATTTTAAAAGATAAAAATGTTGTTTTTACAGGTAATTTTGACACTGAAAAACAA
    GATTTAATGATATTAACTAGAAAGAAAGGAGCTTATATCAGAAGTGACGTAACTGCAAAA
    ACAGATATTTTAGTCGAAGGTGTTCAAGATGATAAATATAAAGATGTGAACGGACTAGTT
    TCAAAACAACGAAAAGCTCGAGAATATGTTGGAAATGGTGCAAAAATTCAATTTTTAAAT
    GAAGAAGACTTAATAAATTTAATAAAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS11090 [old locus tag: NWMN_1922 ]
  • symbol: NWMN_RS11090
  • description: ATP-dependent helicase
  • length: 310
  • theoretical pI: 9.1498
  • theoretical MW: 35900.4
  • GRAVY: -0.280645

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairputative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 62.5)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 62.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 54.2)
    Genetic information processingDNA metabolismDegradation of DNAexonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 42.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA ligase, NAD-dependent (TIGR00575; EC 6.5.1.2; HMM-score: 22.9)
  • TheSEED:
  • PFAM:
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 45.6)
    BRCT-like (CL0459) BRCT; BRCA1 C Terminus (BRCT) domain (PF00533; HMM-score: 16.4)
    RNase_H (CL0219) DUF5051; 3' exoribonuclease, RNase T-like (PF16473; HMM-score: 15.4)
    DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 14.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.137
    • Ymax_pos: 42
    • Cmax: 0.119
    • Cmax_pos: 26
    • Smax: 0.265
    • Smax_pos: 36
    • Smean: 0.122
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446682336 NCBI
  • RefSeq: WP_000759682 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINPPNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNTNNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKMLISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS11090 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]