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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0029 [new locus tag: SA_RS00300 ]
  • pan locus tag?: SAUPAN000059000
  • symbol: pre
  • pan gene symbol?: pre
  • synonym:
  • product: plasmid recombination enzyme

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0029 [new locus tag: SA_RS00300 ]
  • symbol: pre
  • product: plasmid recombination enzyme
  • replicon: chromosome
  • strand: -
  • coordinates: 38428..39690
  • length: 1263
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTTATGCAGTTTGTAGAATGCAAAAAGTGAAATCAGCTGGACTAAAAGGCATGCAA
    TTTCATAATCAAAGAGAGCGAAAAAGTAGAACGAATGATGATATTGACCATGAGCGAACA
    CGTGAAAATTATGATTTGAAAAATGATAAAAATATTGATTACAACGAACGTGTCAAAGAA
    ATTATTGAATCACAAAAAACAGGTACAAGAAAAACGAGGAAAGATGCTGTTCTTGTAAAT
    GAGTTGCTAGTAACATCTGACCGAGATTTTTTTGAGCAACTGGATCCAGGAGAACAAAAA
    CGATTTTTTGAGGAAAGTTATAAATTATTTTCCGAACGATATGGCAAGCAAAATATTGCT
    TATGCAACAGTTCATAATGATGAGCAAACCCCTCACATGCATTTAGGTGTTGTGCCTATG
    CGTGATGGAAAACTGCAAGGAAAAAATGTGTTTAATCGTCAAGAACTGTTATGGCTACAA
    GATAAATTCCCCGAGCATATGAAAAAACAGGGTTTTGAGTTGAAGCGTGGTGAACGTGGC
    TCTGACCGTAAACATATTGAGACAGCTAAATTTAAAAAACAAACTTTGGAAAAAGAGATT
    GATTTTCTAGAAAAAAATTTAGCAGTTAAAAAAGATGAATGGACTGCTTATAGCGATAAA
    GTTAAATCAGATTTAGAAGTACCAGCGAAACGACACATGAAAAGTGTTGAAGTGCCAACG
    GGTGAAAAGTCTATGTTTGGTTTGGGAAAAGAAATAATGAAAACAGAAAAGAAACCAACC
    AAAAATGTTGTTATATCGGAGCGTGATTATAAAAACTTAGTGACTGCTGCGAGAGATAAC
    GATAGGTTAAAACAGCATGTTAGAAATCTCATGAGTACTGATATGGCGAGAGAATATAAA
    AAATTAAGTAAAGAACATGGGCAAGTTAAAGAAAAATATAGTGGTCTTGTAGAGCGATTT
    AATGAAAATGTAAATGATTATAATGAGTTGCTTGAAGAAAACAAGTCTTTAAAGTCTAAA
    ATAAGCGATTTAAAGCGTGATGTGAGTTTAATCTATGAAAGCACTAAGGAATTCCTTAAG
    GAACGTACAGACGGCTTAAAAGCCTTTAAAAACGTTTTTAAGGGGTTTGTAGACAAGGTA
    AAGGATAAAACAGCACAATTCCAAGAAAAACACGATTTAGAACCTAAAAAGAACGAATTT
    GAACTAACTCATAACCGAGAGGTAAAAAAAGAACGAAGTCGAGATCAGGGAATGAGTTTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0029 [new locus tag: SA_RS00300 ]
  • symbol: Pre
  • description: plasmid recombination enzyme
  • length: 420
  • theoretical pI: 9.97102
  • theoretical MW: 49660
  • GRAVY: -1.17738

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsPlasmid functionsintegrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 5.8)
  • TheSEED:  
    Plasmid recombination enzyme 
  • PFAM:
    Rep (CL0169) Mob_Pre; Plasmid recombination enzyme (PF01076; HMM-score: 246.5)
    no clan definedIncA; IncA protein (PF04156; HMM-score: 16.9)
    Sec34; Sec34-like family (PF04136; HMM-score: 10.8)
    HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 8.7)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 6.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.224
    • Ymax_pos: 14
    • Cmax: 0.118
    • Cmax_pos: 14
    • Smax: 0.621
    • Smax_pos: 7
    • Smean: 0.426
    • D: 0.303
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob. Agents Chemother.: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] (P p)