From AureoWiki
Jump to: navigation, search
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0077 [new locus tag: SA_RS00525 ]
  • pan locus tag?: SAUPAN000742000
  • symbol: SA0077
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0077 [new locus tag: SA_RS00525 ]
  • symbol: SA0077
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 85585..87093
  • length: 1509
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    TTGAATGAAGAAATCTTACGCGAAGTAGCAGATATTTTTATAGGTGATGACAGAGATAGC
    ATTTATGATTATAAAACTGGGAACGAATTAGTGAGGTTTTTTAATCATTACTTTAATAAA
    GGGGACATATATCAGGCTCCGTTTCCATCTAGATGGCTATATGTTGTGAAACATTTGCAA
    ACTCTGATTCAGGAGAGAAAGATCAATCAATTTTTCACATTAATTTTAAGCAATCACTAT
    ATTAAGTATGAACTGAAAATTGACGAGGTTGAAGCAGCAAAGCAGGCTGCTAAAGCACTT
    AAGTTGTTCAACAAAAGATTAAATCATTATGGGTACTACATAACAGGAACTAACAATGCT
    AGATATTTTATGGATAAGGATGAAGATACGGAGTCTATTGGTTATGGAGGGTATGCGAAT
    ATTTATTTACAGAAGTCTACAGGTCTTGCTGTAAAAAAATTGAAAGAGGAGTATCTTACT
    GATTCTTCAATTAAAAGTAGGTTTAAGAGAGAATTTGATCTCACTAAATCTTTTGATACA
    AATCCATTGTTCATTAATGTGTTTGAATTTAATGAATCAGATTATTCATACACGATGGAG
    TTAGCTGATGAAACTTTGAAAGATTACATTGAAAGCAAGACAATTAGTGAGCTAGAAAAA
    GTAAAGATAATAATGAAAATTTTAAAAGCGATGAGTCAAGCACATAGTGAAAATAAAATA
    CACAGAGATATCAGTTCTAAAAATGTATTAATGTTTAGAGGAAAAGTCAAAATATCAGAC
    TTAGGATTAGGTAAAAACCTTGATGAAATTCATTCGCATCAAACCTTTGATACAAACGGT
    GTAGGACAATATAAATACTGTGCACCGGAACAGATGTATAGTTTAAAACAAGCAGATAAA
    CAATCTGATGTTTTTAGTTTAGGAAGATTGATAAATTTTATTATGACTGGAAATGTAGTT
    AACAACCATCACCTATTTAGAGGTGTATCTGATAAGGCTACGAACAGTAGTAAAGAATAC
    AGATTTGAAGATGCAAATGAAATGTTGAAAATGCTGCAGAGAATTTTAGAGTATCACAGT
    AGTGCAAAGCACGTCGAAAAATGTCAAGAAAAGCTGAAAAGAGGAGTGTTTGATGATGAA
    AGCGAAGAATTTATTATGACACGAAGTGATGAACAATTATGTCAAATGGTTCTAAGTTCT
    AATAATAATGAGCAAGCGTGTTTAATTCGTTATATGCAAAAAAACGAATCTTCAGCATGT
    GATTTAATAGAGAGTATTAATAGAAAGTATCAAGAGTTTTGTGGAAGGTTTGAAGACTAC
    GATCCTTTTGCTAAATTAGCATATATGATTTTATGTAATAACTTCAGTTATAGAGTGAAT
    GAAACAGCAGCTAGAGTGCTAAATTATGTTGCTTGGTCTGTAAATAGATTTTCGGCACAA
    GACTTAATTAAAGGTTTAATTAATAGAGGAGTGGAGCCTTTGATTGAAGAAAAATTAAAG
    GACAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0077 [new locus tag: SA_RS00525 ]
  • symbol: SA0077
  • description: hypothetical protein
  • length: 502
  • theoretical pI: 6.65373
  • theoretical MW: 58731.2
  • GRAVY: -0.571116

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistanceTOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 44.2)
    Unknown functionGeneralKae1-associated kinase Bud32 (TIGR03724; EC 2.7.-.-; HMM-score: 31.6)
    RHS repeat-associated core domain (TIGR03696; HMM-score: 14.6)
  • TheSEED:  
    FIG01109972: hypothetical protein 
  • PFAM:
    PKinase (CL0016) Pkinase; Protein kinase domain (PF00069; HMM-score: 93.8)
    Pkinase_Tyr; Protein tyrosine kinase (PF07714; HMM-score: 69.3)
    P-loop_NTPase (CL0023) AAA_14; AAA domain (PF13173; HMM-score: 17)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.096
    • Ymax_pos: 69
    • Cmax: 0.106
    • Cmax_pos: 46
    • Smax: 0.115
    • Smax_pos: 70
    • Smean: 0.055
    • D: 0.08
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNEEILREVADIFIGDDRDSIYDYKTGNELVRFFNHYFNKGDIYQAPFPSRWLYVVKHLQTLIQERKINQFFTLILSNHYIKYELKIDEVEAAKQAAKALKLFNKRLNHYGYYITGTNNARYFMDKDEDTESIGYGGYANIYLQKSTGLAVKKLKEEYLTDSSIKSRFKREFDLTKSFDTNPLFINVFEFNESDYSYTMELADETLKDYIESKTISELEKVKIIMKILKAMSQAHSENKIHRDISSKNVLMFRGKVKISDLGLGKNLDEIHSHQTFDTNGVGQYKYCAPEQMYSLKQADKQSDVFSLGRLINFIMTGNVVNNHHLFRGVSDKATNSSKEYRFEDANEMLKMLQRILEYHSSAKHVEKCQEKLKRGVFDDESEEFIMTRSDEQLCQMVLSSNNNEQACLIRYMQKNESSACDLIESINRKYQEFCGRFEDYDPFAKLAYMILCNNFSYRVNETAARVLNYVAWSVNRFSAQDLIKGLINRGVEPLIEEKLKDN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]