PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0402 [new locus tag: SA_RS02300 ]
• pan locus tag^?: SAUPAN002144000
• symbol: lpl6
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0402 [new locus tag: SA_RS02300 ]
• symbol: lpl6
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 462060..462854
• length: 795
• essential: no DEG

⊟Accession numbers

• Gene ID: 1123186 NCBI
• RefSeq: NP_373653 NCBI
• BioCyc: see SA_RS02300
• MicrobesOnline: 102679 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATGGGTTATTTAAAAAGACTTGTATTGTACATAGTTATTATGGTTATGAGTGTTTTT
  ATAATAGGTTGTGATAAATCAAGCGATACTGCAGAAAATCCAAAAGAAGGTTCAAAAGAA
  GCACAAATTAAAAAGAGTTTTTCGAAAACGTTAGATATGTATCCAATTAAGAATCTCGAG
  AATCTATATGACAAAGAAGGATATCGTGATGGCGAATTTAAAAAAGGTGATAAAGGGACT
  TGGACAATATCAACAGATTTTGCTAAAAGCAACAAGCAAGGTGAAATGAATAGCGAAGGT
  ATGGTACTACATTTTAATAGAAATACGAGGACAGCAACAGGATATTATACTGTAAGAACA
  ACTTATGATGAAGTGGATAAGTTGGCACGCGAAAAAAAATATCGTGTTGAATTTAAAAAC
  AATAAGATAGTTCTATTGGACAAAGTAGAAGATGAAAATCTTAAACAAAAAATAGAAAAT
  TTTAAATTTTTCGGGCAATATGCCGATTTTAAAGACTTGAAAAATTATAAAAATGGAAGA
  ATATCTAGCAATGAAAATGTTCCTTATTATGAAGCAGAGTACAAAAGGAATAATAGTGAT
  GGAAATGTAAAAAAACTTAGAGAAAAGTACCCAATTACAACCAAGCAGTCTCCAATATTA
  AAACTGCATATAGACGGCGATATTAAAGGTAGCTCAGTTGGATATAAACAGATAGAATAC
  ACATTTTCTAAGGAGAAAGATGATGAGACTTTTATGAGTGATTTTTTGAACTTCGGTCCA
  TCACACAGTAAATAG
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⊟Protein

⊟General

• locus tag: SA0402 [new locus tag: SA_RS02300 ]
• symbol: Lpl6
• description: hypothetical protein
• length: 264
• theoretical pI: 9.50159
• theoretical MW: 30759.6
• GRAVY: -0.905682

⊟Function

• TIGRFAM:
  Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 353)
  and 2 more
  Cell structure Cell envelope Other lipoprotein, tandem type (TIGR04389; HMM-score: 11.1)
  surface glycoprotein signal peptide (TIGR04207; HMM-score: 9.6)
• TheSEED  :
  • FIG01107840: hypothetical protein
• PFAM:
  LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 355.4)
  and 2 more
  no clan defined PsbI; Photosystem II reaction centre I protein (PSII 4.8 kDa protein) (PF02532; HMM-score: 17.1)
  FidL_like; FidL-like putative membrane protein (PF15941; HMM-score: 14.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 4.6
  • Cellwall Score: 2.48
  • Extracellular Score: 2.92
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: VFIIGCD
• SignalP: Signal peptide LIPO(Sec/SPII) length 23 aa
  • SP(Sec/SPI): 0.001058
  • TAT(Tat/SPI): 0.000055
  • LIPO(Sec/SPII): 0.998656
  • Cleavage Site: CS pos: 23-24. IIG-CD. Pr: 0.9991
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15926120 NCBI
• RefSeq: NP_373653 NCBI
• UniProt: Q7A7G1 UniProt

⊟Protein sequence

• MMGYLKRLVLYIVIMVMSVFIIGCDKSSDTAENPKEGSKEAQIKKSFSKTLDMYPIKNLENLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKQGEMNSEGMVLHFNRNTRTATGYYTVRTTYDEVDKLAREKKYRVEFKNNKIVLLDKVEDENLKQKIENFKFFGQYADFKDLKNYKNGRISSNENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPILKLHIDGDIKGSSVGYKQIEYTFSKEKDDETFMSDFLNFGPSHSK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications