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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1609 [new locus tag: SA_RS09060 ]
  • pan locus tag?: SAUPAN004503000
  • symbol: pckA
  • pan gene symbol?: pckA
  • synonym:
  • product: phosphoenolpyruvate carboxykinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1609 [new locus tag: SA_RS09060 ]
  • symbol: pckA
  • product: phosphoenolpyruvate carboxykinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1844783..1846375
  • length: 1593
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACG
    TCACATTTTCAACTTTCGACGACACAACTTTATAATAAAATCTTAGACAATAACGAAGGG
    GTATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAAAATATACTGGTCGTTCGCCT
    AAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
    AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTA
    GATAAAAAAGATGAACTATACGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATG
    TTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATTTATTTGCTAAAAATATGTTT
    ATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
    GCACCACATTTTAAAGCAGATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATT
    ATTTCATTTAAACACAAAGTCATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAA
    AAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGCAAGATATTATGAGCATGCAT
    TGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
    GGTAAAACAACCTTATCGGCTGACCCACACCGTAAACTAATCGGTGATGATGAACACGGA
    TGGAATAAAAACGGGGTCTTTAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTT
    TCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAATATGGTGCAATTTTAGAGAAC
    ACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGACAATCGTTATACAGAAAACACG
    CGTGCCGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCAAAAGCAGCACAT
    CCAAATACAATTATTTTCTTAACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAG
    TTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTTTCACTTCTAAATTAGCTGGT
    ACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGAGCACCGTTC
    TTCCCGTTACACCCTACTGTTTACGCTGATTTATTAGGTGAACTTATCGATTTACATGAT
    GTTGATGTTTATCTTGTTAATACTGGATGGACTGGCGGAAAATATGGTGTAGGACGTAGA
    ATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGATTTCTGGCAAATTGAAAAAT
    GCAGAATATACAAAAGATAGTACGTTTGGTTTAAGCATTCCTGTAGAAATTGAAGATGTA
    CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAA
    GCAGAAGATTTAATTCAACGTTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAA
    CATATTGCTGAAAAAGGTAGCTTCAACAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1593

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1609 [new locus tag: SA_RS09060 ]
  • symbol: PckA
  • description: phosphoenolpyruvate carboxykinase
  • length: 530
  • theoretical pI: 6.01708
  • theoretical MW: 59376.9
  • GRAVY: -0.388491

Function[edit source | edit]

  • reaction:
    EC 4.1.1.49?  ExPASy
    Phosphoenolpyruvate carboxykinase (ATP)ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphoenolpyruvate carboxykinase (ATP) (TIGR00224; EC 4.1.1.49; HMM-score: 792.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 11.4)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPyruvate metabolism I: anaplerotic reactions, PEP Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) 
    One-carbon MetabolismSerine-glyoxylate cycle Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) 
  • PFAM:
    PEP-carboxyk (CL0374) PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PF01293; HMM-score: 735)
    PEPCK_C; Phosphoenolpyruvate carboxykinase C-terminal P-loop domain (PF00821; HMM-score: 15.3)
    P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.139
    • Ymax_pos: 11
    • Cmax: 0.129
    • Cmax_pos: 51
    • Smax: 0.226
    • Smax_pos: 7
    • Smean: 0.196
    • D: 0.162
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)