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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1724 [new locus tag: SA_RS09895 ]
  • pan locus tag?: SAUPAN004937000
  • symbol: purB
  • pan gene symbol?: purB
  • synonym:
  • product: adenylosuccinate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1724 [new locus tag: SA_RS09895 ]
  • symbol: purB
  • product: adenylosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 1973966..1975261
  • length: 1296
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTGAACGCTATTCTAGAGAAGAAATGTCTAATATTTGGACTGATCAAAATCGCTAT
    GAAGCATGGTTAGAAGTGGAAATTTTAGCATGTGAAGCATGGAGTGAATTAGGACATATA
    CCGAAAGCTGACGTACAAAAAATTCGTCAAAACGCAAAGGTAAATGTCGAACGTGCACAA
    GAAATTGAACAAGAAACGCGCCATGATGTTGTAGCCTTTACTAGACAAGTTTCTGAAACG
    CTAGGTGAAGAACGTAAGTGGGTACATTATGGTTTAACTTCTACTGATGTTGTAGATACA
    GCTTTAAGTTTCGTTATTAAACAAGCAAATGACATTATTGAAAAAGATTTAGAAAGATTT
    ATCGACGTTTTAGCTGAAAAAGCAAAAAATTATAAATATACATTGATGATGGGACGTACA
    CATGGAGTGCATGCAGAACCAACTACATTTGGTGTCAAAATGGCATTGTGGTACACGGAA
    ATGCAACGTAACTTACAACGTTTCAAACAAGTAAGAGAAGAAATTGAAGTAGGGAAAATG
    AGTGGTGCAGTAGGTACTTTTGCTAACATTCCTCCTGAAATAGAAAGTTATGTGTGTAAA
    CATTTAGGTATTGGCACAGCGCCTGTATCAACGCAAACATTGCAACGCGATCGACATGCA
    TACTATATTGCGACATTAGCATTAATCGCGACGTCACTAGAAAAATTTGCCGTTGAAATT
    CGTAACCTTCAAAAAACCGAAACAAGAGAAGTTGAAGAAGCATTTGCAAAAGGCCAAAAA
    GGTTCATCTGCAATGCCTCATAAACGAAATCCAATTGGTTCTGAAAATATCACAGGTATT
    TCAAGAGTCATTCGTGGTTATATTACTACAGCTTATGAGAATGTGCCATTATGGCATGAA
    AGAGATATTTCTCATTCTTCTGCTGAGCGTATTATGTTACCAGATGTTACAATAGCACTA
    GATTATGCATTGAATCGTTTCACAAATATTGTGGACCGTTTAACTGTATTTGAAGATAAT
    ATGCGTAATAACATAGATAAAACATTTGGTCTGATTTTCTCACAACGTGTCTTATTAGCA
    TTAATTAATAAAGGTATGGTTCGTGAAGAAGCATACGACAAAGTACAACCAAAAGCGATG
    ATATCTTGGGAAACGAAAACACCTTTCCGTGAATTAATCGAACAAGATGAGTCTATTACA
    AGTGTTTTAACAAAAGAAGAATTAGATGAATGTTTCGATCCTAAACATCATTTGAATCAA
    GTTGACACTATTTTCGAACGCGCTGGATTAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1296

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1724 [new locus tag: SA_RS09895 ]
  • symbol: PurB
  • description: adenylosuccinate lyase
  • length: 431
  • theoretical pI: 5.6693
  • theoretical MW: 49603
  • GRAVY: -0.435963

Function[edit source | edit]

  • reaction:
    EC 4.3.2.2?  ExPASy
    Adenylosuccinate lyaseN6-(1,2-dicarboxyethyl)AMP = fumarate + AMP(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 581.4)
    MetabolismEnergy metabolismOther3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 196)
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 85.6)
    MetabolismEnergy metabolismAmino acids and aminesaspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 62.6)
    MetabolismEnergy metabolismTCA cyclefumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 56.7)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesDe Novo Purine Biosynthesis Adenylosuccinate lyase (EC 4.3.2.2) 
    PurinesPurine conversions Adenylosuccinate lyase (EC 4.3.2.2) 
    De Novo Purine Biosynthesis SAICAR lyase (EC 4.3.2.2) 
  • PFAM:
    no clan definedLyase_1; Lyase (PF00206; HMM-score: 253.4)
    ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 92.5)
    ASL_C; Adenylosuccinate lyase C-terminal (PF08328; HMM-score: 15.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 44
    • Cmax: 0.128
    • Cmax_pos: 44
    • Smax: 0.117
    • Smax_pos: 42
    • Smean: 0.058
    • D: 0.083
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: PurR
    PurR (TF) important in Purine metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]